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Preparation method of scanning electron microscope sample for polymer vesicae

A scanning electron microscope and polymer technology, which is applied in the detection field, can solve the problems of polymer vesicle morphology, size information distortion, and influence on observation, and achieve the effects of easy-to-obtain dyes, easy focus adjustment, and high image resolution

Inactive Publication Date: 2016-06-29
SOUTH CHINA UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there are certain disadvantages in the preparation method of polymersomes by spraying gold for SEM samples: because gold particles have a certain volume, it will cover the fine structure of the surface morphology of some polymersomes, and it will affect the accuracy of nanometer-sized vesicles. Therefore, the observation of polymer vesicles will cause distortion of the shape and size information of polymer vesicles; in addition, gold spray equipment is not commonly used in general laboratories, and it often takes a certain amount of time to prepare samples by spraying gold.

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  • Preparation method of scanning electron microscope sample for polymer vesicae
  • Preparation method of scanning electron microscope sample for polymer vesicae
  • Preparation method of scanning electron microscope sample for polymer vesicae

Examples

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Embodiment 1

[0027] (1) Preparation of dye solution: Weigh 37.5mg of sodium phosphotungstate and add it to 5mL of water, and ultrasonicate for 10 minutes (ultrasonic power is 50W, ultrasonic frequency is 300kHz) to completely dissolve it to obtain phosphotungsten with a concentration of 0.75% w / w Sodium acid stain solution.

[0028] (2) Sample stage preparation: Paste conductive glue on the aluminum circular sample stage of the scanning electron microscope, and then attach a circular slide glass with a diameter of 12 mm.

[0029] (3) Staining and drying of samples: drop 50 μL PEG-PCL block copolymer nanoscale polymer vesicle solution on the glass slide, and then add 10 μL dye solution dropwise; use filter paper to lightly touch and absorb the excess mixed solution, The substrate is covered with a thin layer of mixed solution, and dried at room temperature to form a film to obtain a scanning electron microscope sample, at which point scanning electron microscopy can be photographed. The sc...

Embodiment 2

[0031] (1) Preparation of dye solution: Weigh 12.5mg of sodium phosphotungstate and add it to 5mL of water, and ultrasonicate for 5 minutes (ultrasonic power is 60W, ultrasonic frequency is 300kHz) to completely dissolve it to obtain phosphotungsten with a concentration of 0.25% w / w Sodium acid stain solution.

[0032] (2) Sample stage preparation: Paste conductive glue on the aluminum circular sample stage of the scanning electron microscope, and then attach a mica sheet with a diameter slightly smaller than the area of ​​the sample stage.

[0033] (3) Staining and drying of samples: drop 50 μL PEG-PCL block copolymer nanoscale polymer vesicle solution on the glass slide, and then add 10 μL dye solution dropwise; use filter paper to lightly touch and absorb the excess mixed solution, The substrate is covered with a thin layer of mixed solution, and dried at room temperature to form a film to obtain a scanning electron microscope sample, at which point scanning electron micros...

Embodiment 3

[0035] (1) Preparation of dye solution: Weigh 37.5mg of sodium phosphotungstate and add it to 5mL of water, stir evenly (stirring speed is 500r / min, stirring time is 30min), and obtain sodium phosphotungstate dyeing with a concentration of 0.75% w / w agent solution.

[0036] (2) Sample stage preparation: Paste conductive glue on the aluminum circular sample stage of the scanning electron microscope, and then attach a piece of aluminum foil with a diameter slightly smaller than the area of ​​the sample stage.

[0037] (3) Sample staining and drying: Drop 50 μL of nanoscale polymer vesicle solution composed of PEG-PLA block copolymer on the glass slide, and then add 10 μL of dye solution dropwise; use filter paper to lightly touch and absorb the excess mixed solution, The substrate is covered with a thin layer of mixed solution, and dried at room temperature to form a film to obtain a scanning electron microscope sample, at which point scanning electron microscopy can be photogra...

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Abstract

The invention belongs to the technical field of detection and discloses a preparation method of a scanning electron microscope sample for polymer vesicae. The preparation method comprises the following steps: weighing heavy metal salt, and stirring or uniformly ultrasonically dissolving the heavy metal salt in a solvent, so as to obtain a coloring agent solution; sticking a conducting adhesive to a sample table of a scanning electron microscope, and adhering a layer of substrate material to the conducting adhesive; dropwise adding a polymer vesica solution and the obtained coloring agent solution to the surface of the substrate material, and drying to form a film, so as to obtain the scanning electron microscope sample for the polymer vesicae. Compared with a traditional sample preparation method, the preparation method has the advantages that metal spraying treatment is omitted; and the focusing is easy during the observation of a scanning electron microscope, the image resolution of the obtained sample is high, surface details of the polymer vesicae can be adequately maintained, the damaging of the sample in the sample preparation process can be avoided, and spherical structures of the polymer vesicae can be clearly observed.

Description

technical field [0001] The invention belongs to the technical field of detection, and in particular relates to a preparation method of a polymer vesicle scanning electron microscope sample. Background technique [0002] Polymer vesicles are a kind of ordered polymer assembly with a spherical hollow structure formed by the self-assembly of amphiphilic block copolymer molecules. The structure and properties of polymersomes, such as stability, structure and functionality, and membrane permeability, are largely influenced by the properties of block copolymers. In general, the membrane of a polymersome can be divided into three layers: two hydrophilic layers and a hydrophobic layer between the inner and outer hydrophilic layers, which is stabilized by the hydrophilic layers on the inner and outer surfaces. The particle size of polymersomes is generally about 30nm to 300nm, and the size of some giant vesicles can even reach tens of microns. Compared with low-molecular-weight lip...

Claims

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Application Information

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IPC IPC(8): G01N1/28G01N1/30
CPCG01N1/28G01N1/30
Inventor 王林格刘思琦
Owner SOUTH CHINA UNIV OF TECH
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