Phycocyanin separating and enriching device

Abstract

The invention relates to a phycocyanin separating and enriching device based on free flow electrophoresis. The phycocyanin separating and enriching device comprises a free flow electrophoresis main body separating device, a collecting device, an infusion pump and a pipeline. The free flow electrophoresis main body separating device is different from a common free flow electrophoresis device, multiple injection ports are formed, but a buffer solution inlet is not formed, and the upper end is arc-shaped, so that a stagnation phenomenon is weakened. By utilizing the characteristics that free flow electrophoresis can realize continuous separation and different electric matters have different migration rates in an electric field, phycocyanin crude extract and even protoplasm of cyanobacteria cells after disruption can directly enter the device to be enriched and separated, and an effective way is provided for rapid and efficient large-scale phycocyanin preparation.

Description

technical field [0001] The invention relates to the technical field of material separation, in particular to a phycocyanin separation and enrichment device. Background technique [0002] As a product of eutrophication in water bodies, cyanobacteria have a huge impact on water quality. At present, mechanical and manual salvage are the main measures to deal with the outbreak of blue-green algae, but if the salvaged blue-green algae are not handled properly, it will cause secondary pollution. The processing and utilization of blue-green algae is mainly through biogas and fertilizer, the added value of the product is low, and the economic benefit is not high. The phycocyanin contained in blue-green algae is as high as more than 5%, and the price is extremely high. All phycocyanin of fluorescent reagent grade is monopolized by foreign countries. Separating high-purity phycocyanin from blue-green algae can not only reduce environmental pollution, but also generate higher economi...

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Problems solved by technology

[0003] Commonly used phycocyanin separations include repeated freeze-thaw methods, chemical reagent treatment methods, swelling methods, ultrasonic methods, etc. The resulting phycocyanin has a low purity, while the high-purity phycocyanin requires further centrifugation, ammonium sulfate, etc. Gradient salting out and hydroxyapatite column separation, many steps, complex operation and high cost, limit the large-scale production of phycocyanin

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