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Snake abdomen scale lower fat and extraction method and application thereof

An extraction method and snake belly technology, which is applied in the field of animal oil extraction, can solve the problems of low purity of extracted products, limit the application of snake belly fat, and low yield, and achieve the effects of improving skin lubricity, low saponification value, and increasing water content

Inactive Publication Date: 2016-07-06
克劳丽化妆品股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using the above method to extract snake belly fat, not only the yield is low, but also the purity of the extracted product is low, thus limiting the application of snake belly fat

Method used

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  • Snake abdomen scale lower fat and extraction method and application thereof
  • Snake abdomen scale lower fat and extraction method and application thereof
  • Snake abdomen scale lower fat and extraction method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The extraction method of the subscale fat of the snake belly of the present embodiment comprises the following steps:

[0034] (1) the belly skin of the black-sharp snake snake is ground to 0.8mm, and the belly skin of the scale snake is ground;

[0035] (2) Add 500 g of the minced scaled snake belly skin to 5000 g of water, first use 5 g of keratinase for 240 min at pH 7.5 and 50° C. Then use 1g alkaline protease to carry out enzymolysis for 120min to obtain the enzymatic hydrolysis product of scaled snake belly skin;

[0036] (3) centrifuging the enzymatic hydrolyzate of snake belly skin, taking the supernatant and separating oil from water to obtain the crude snake belly fat;

[0037] (4) Add the crude product of snake belly fat to 1 weight-fold amount of ether, microwave extraction for 5 minutes under the condition of 500w, concentrate at 40°C, remove the ether by distillation, and the concentrate is snake belly fat (light yellow , clear liquid, no fishy smell, th...

Embodiment 2

[0040] The extraction method of the subscale fat of the snake belly of the present embodiment comprises the following steps:

[0041] (1) mincing the belly skin of Agkistrodon viper scaled snake to 0.5mm, to obtain the minced thing of scaled snake belly skin;

[0042] (2) Add 1,000 g of the minced scaled snake belly skin to 15,000 g of water, first use 5 g of keratinase for 300 min at pH 7.2 and 45° C. Carry out enzymolysis with 8g alkaline protease again 60min, obtain the enzymolysis product of the belly skin of scaled snake;

[0043] (3) centrifuging the enzymatic hydrolyzate of snake belly skin, taking the supernatant and separating oil from water to obtain the crude snake belly fat;

[0044] (4) Add the crude product of snake belly fat to 0.5 times the weight of ether, microwave extraction under the condition of 700w for 6min, concentrate at 45°C, remove the ether by distillation, and the concentrate is snake belly fat (light yellow , clear liquid, no fishy smell, the yi...

Embodiment 3

[0047] The extraction method of the subscale fat of the snake belly of the present embodiment comprises the following steps:

[0048] (1) mincing the belly skin of the water snake scaled snake to 1mm, to obtain the minced thing of the scaled snake belly skin;

[0049] (2) Add 500 g of the minced scaled snake belly skin to 10000 g of water, first use 1 g of keratinase for 280 min at pH 7.0 and 48 °C, and then hydrolyze it at pH 7.2 and 52 °C Then use 5g of alkaline protease or compound protease to carry out enzymolysis for 90 minutes to obtain the enzymatic hydrolysis product of the scaled snake belly skin;

[0050] (3) centrifuging the enzymatic hydrolyzate of snake belly skin, taking the supernatant and separating oil from water to obtain the crude snake belly fat;

[0051] (4) Add the crude snake belly fat to 2 times the weight of petroleum ether, microwave extraction for 2 minutes under the condition of 1000w, concentrate at 42°C, remove the petroleum ether by distillation...

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Abstract

The invention relates to snake abdomen scale lower fat and an extraction method and application thereof.The extraction method includes following steps: mincing abdomen skin of a scaled snake to obtain a scaled snake abdomen skin minced product; adding the scaled snake abdomen skin minced product into water, and protease for enzymolysis to obtain a scaled snake abdomen skin enzymolysis product; centrifuging the scaled snake abdomen skin enzymolysis product, and taking supernate for oil-water separation to obtain a snake abdomen scale lower fat crude product; adding the snake abdomen scale lower fat crude product into an ether solvent, and performing microwave extraction and concentration to obtain the snake abdomen scale lower fat.The snake abdomen scale lower fat obtained by the method is high in saturated fatty acid content and low in unsaturated fatty acid content, iodine value, acid value and saponification value, friction coefficient of human hand back skin is increased remarkably, water content of the human hand back skin is increased remarkably, and the snake abdomen scale lower fat has a moisturizing function; the snake abdomen scale lower fat has efficacy of protecting skin, improving skin elasticity, nourishing skin and improving skin smoothness when being used for preparing products having skin protecting effect.

Description

technical field [0001] The invention belongs to the field of animal oil extraction, and in particular relates to snake belly fat and its extraction method and application. Background technique [0002] Snake oil is rich in natural active substances and antibacterial ingredients. It has super penetrating power and can directly penetrate into human skin to promote skin cell metabolism, thereby enhancing the immune regulation function of the skin. In addition, snake oil is also a nutritious, health-care substance that can regulate endocrine disorders, laxatives, beautify the skin, prevent skin aging, and prevent vascular sclerosis. [0003] However, the existing snake oil is mainly visceral fat mixture, and its composition and structure are not clear. It mainly contains 12 kinds of fatty acids, such as unsaturated fatty acids such as linoleic acid and linolenic acid, and glycerol palmitic acid. Among them, linoleic acid, the unsaturated fatty acid with the most content, may be...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/02C11B1/00C11B3/00A61K8/92A61Q19/00
CPCA61K8/925A61Q19/00C11B1/00C11B3/005C11B3/006
Inventor 李赟高
Owner 克劳丽化妆品股份有限公司
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