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A screening method for varieties of hyperaccumulator heavy metal plants

A screening method and heavy metal technology, applied in soil treatment and agricultural fields, can solve the problems of poor result stability, large required area, and heavy workload, and achieve the effects of small occupied area, small biological volume, and reduced workload

Active Publication Date: 2018-03-27
JIANGXI AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has a large workload, large required area, high cost, long cycle, and poor stability of results.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Embodiment 1 of the present invention provides a kind of hyperaccumulation rapeseed screening method, comprises the following steps,

[0045] a. Select 400 seeds, use 4g / kg sodium hypochlorite to soak the seeds for 8 minutes, then pour out the sodium hypochlorite, and clean the seeds twice with pure water continuously;

[0046] b. Using cadmium chloride 2 / 5 hydrate and pure water, set up cadmium tests with 7 concentration gradients of 10mg / kg, 20mg / kg, 40mg / kg, 60mg / kg, 80mg / kg, 100mg / kg, and 120mg / kg solution;

[0047] c. Divide 400 rapeseeds into 8 parts and place them in 8 petri dishes, numbered 1-8 in sequence, with 50 rapeseeds in each petri dish, among which, add 3ml dropwise to No. 1-7 petri dishes For the cadmium test solution with a concentration of 10-120mg / kg, keep the No. 8 Petri dish and add the same amount of water dropwise, as a control group, and set up 2 repetitions;

[0048] d. Place all the petri dishes in step c at 22°C, in an environment where the...

Embodiment 2

[0053] Embodiment 2 of the present invention provides a method for screening hyperaccumulator heavy metal plants, comprising the following steps,

[0054] a. Select 315 seeds, use 6g / kg potassium permanganate solution to soak the seeds for 12 minutes, then pour out the potassium permanganate solution, and clean the seeds 4 times with pure water continuously;

[0055]b. Using cadmium nitrate and ultrapure water, set up cadmium test solutions with 6 concentration gradients of 30mg / kg, 50mg / kg, 70mg / kg, 90mg / kg, 110mg / kg, and 130mg / kg;

[0056] c. Divide 315 rapeseeds into 7 parts and place them in 7 petri dishes, numbered 1-7 in turn, with 45 rapeseeds in each petri dish, among which 5ml is added dropwise to No. 1-6 petri dishes For the cadmium test solution with a concentration of 30-130 mg / kg, keep the No. 7 Petri dish and add the same amount of water dropwise, as a control group, and set up 3 repetitions;

[0057] d. Place all the petri dishes in step c at 25°C, in an enviro...

Embodiment 3

[0062] Embodiment 3 of the present invention provides a method for screening hyperaccumulator heavy metal plants, comprising the following steps,

[0063] a. Select 400 seeds of Zhongshuang No. 11, soak the seeds with 75% alcohol for 5 minutes, pour out the alcohol, then soak the seeds with 5g / kg sodium hypochlorite for 10 minutes, pour out the sodium hypochlorite, and then wash the seeds with pure water 3 times, and then wash 1 time with ultrapure water;

[0064] b. Using cadmium chloride 2 / 5 hydrate and pure water, set up cadmium tests with 7 concentration gradients of 10mg / kg, 20mg / kg, 40mg / kg, 60mg / kg, 80mg / kg, 100mg / kg, and 120mg / kg solution;

[0065] c. Divide 400 rapeseed seeds into 8 parts, place them in 8 petri dishes with 2 layers of filter paper, numbered 1-8 in sequence, and each petri dish has 50 rapeseed seeds, of which No. 1-7 Add 4ml of cadmium test solution with a concentration of 10-120mg / kg dropwise to the petri dish successively, keep No. 8 petri dish and...

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Abstract

The invention provides a method for screening the varieties of heavy metal hyperaccumulation plants and relates to the technical fields of agriculture and soil treatment.The method includes: setting screening standard concentration, selecting the varieties of primary-screening plants, and screening the varieties of the heavy metal hyperaccumulation plants.The method is low in operation workload, small in required area, low in cost, short in selection cycle, good in result stability, high in practicality, and suitable for being widely popularized.

Description

technical field [0001] The invention relates to the fields of agricultural technology and soil treatment technology, in particular to a screening method for varieties of hyperaccumulated heavy metal plants. Background technique [0002] Soil is one of the important resources of human society and the material basis for the development of agriculture, industry and urban modernization. However, due to the strong impact of human activities such as mining and smelting, industrial and traffic emissions, waste dumping, and use of pesticides and fertilizers, soil pollution is becoming more and more serious. In particular, the development and smelting of mineral resources, while providing important strategic resources for the country, has also caused serious consequences such as environmental pollution, geological disasters, and ecological damage. [0003] Among the many soil pollution control methods, compared with the traditional chemical and physical treatment techniques, phytore...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B07C5/34
CPCB07C5/34
Inventor 付东辉朱隆荣肖美丽周庆红
Owner JIANGXI AGRICULTURAL UNIVERSITY
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