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Yeast and culture method and application thereof

A culture method and yeast technology, applied in the field of yeast, can solve the problem that there is no compound biological essential oil and the like

Inactive Publication Date: 2016-07-13
湖北同泽堂生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no precedent for the preparation of compound biological essential oil by yeast fermentation

Method used

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  • Yeast and culture method and application thereof
  • Yeast and culture method and application thereof
  • Yeast and culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Yeast provided by the invention is cultivated according to the following method:

[0036] (1) Insert the freeze-dried preserved bacteria into the sterilized primary seed medium, which contains (g / L): glucose 5, peptone 2, and K 2 HPO 4 0.2, the inoculum size is 2%, and cultivated for 40 hours to obtain the first-grade seed liquid;

[0037] (2) Insert the primary seed liquid obtained in step (1) into the secondary seed medium, which contains (g / L): glucose 10, peptone 4, and K 2 HPO 40.4, the inoculum size was 5%, and cultivated for 30 hours to obtain the secondary seed liquid;

[0038] (3) The secondary seed liquid obtained in step (2) is fermented, and the fermentation medium contains (g / L): sucrose 15, peptone 4, K 2 HPO 4 1. And sodium succinate 1, in the 20th hour of fermentation, add 100ml of 5% sucrose solution in a sterilized mass ratio per liter of fermentation broth.

[0039] The biological essential oil obtained by evaporating the solvent after the produ...

Embodiment 2

[0048] Yeast provided by the invention is cultivated according to the following method:

[0049] (1) Insert the freeze-dried preserved bacteria into the sterilized primary seed medium, which contains (g / L): glucose 10, peptone 4, and K 2 HPO 4 0.5, the inoculation amount was 5%, cultured for 35 hours, and obtained the first-grade seed liquid;

[0050] (2) Insert the primary seed liquid obtained in step (1) into the secondary seed medium, which contains (g / L): glucose 15, peptone 6, and K 2 HPO 4 0.7, the inoculum size was 7%, and cultivated for 25 hours to obtain the secondary seed liquid;

[0051] (3) The secondary seed liquid obtained in step (2) is fermented, and the fermentation medium contains (g / L): sucrose 20, peptone 6, K 2 HPO 4 1.5, and sodium succinate 2, at the 25th hour of fermentation, add 200 ml of 5% sucrose solution that has been sterilized by mass per liter of fermentation broth.

[0052] After the product is extracted with n-hexane, the biological esse...

Embodiment 3

[0056] Yeast provided by the invention is cultivated according to the following method:

[0057] (1) Insert the freeze-dried preserved bacteria into the sterilized primary seed medium, which contains (g / L): glucose 15, peptone 6, growth promoter yeast extract 1g / L, and K 2 HPO 4 0.8, the inoculum size was 8%, and cultured for 20 hours to obtain a first-grade seed liquid; the yeast extract manufacturer is Hubei Angel Yeast Co., Ltd. (CAS No. 8013-01-2).

[0058] (2) Insert the primary seed liquid obtained in step (1) into the secondary seed medium, which contains (g / L): glucose 20, peptone 8, and K 2 HPO 4 1. The inoculum amount is 10%, cultured for 20 hours, and the secondary seed liquid is obtained;

[0059] (3) The secondary seed liquid obtained in step (2) is fermented, and the fermentation medium contains (g / L): sucrose 30, peptone 8, K 2 HPO 4 2. As for sodium succinate 3, in the 30th hour of fermentation, 500 ml of sterilized 5% sucrose solution was added per liter...

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Abstract

The invention discloses a yeast and a culture method and application thereof. The culture method of the yeast comprises the following steps: (1) inoculating a sterilized first-level seed culture medium with lyophilized preserved bacteria, wherein the inoculation amount is 2-8%; and culturing for 20-40 hours to obtain first-level seed liquid; (2) inoculating a second-level seed culture medium with the first-level seed liquid, wherein the inoculation amount is 5-10%; and culturing for 20-30 hours to obtain second-level seed liquid; and (3) performing fermentation culture on the second-level seed liquid; and in the 20th-30th hour of the fermentation, feeding 100-500ml of sterilized sucrose solution in a mass proportion of 5% into each liter of fermentation liquid. The yeast is used for producing biological essential oil by a fermentation method; and the essential oil is similar to rose essential oil in composition and function and is a good substitute of the rose essential oil.

Description

technical field [0001] The invention belongs to the field of biotechnology, and more specifically relates to a yeast, its cultivation method and application. Background technique [0002] Yeast are unicellular fungi that are not a unit of phylogenetic classification. It is a general term for several single-celled fungi such as Ascomycetes and Basidiomycetes. It generally refers to various single-celled fungi that can ferment sugars, and is widely used in fermentation engineering. [0003] Yeast is closely related to industrial fermentation production and human life. In addition to making dough for steamed buns, bread and brewing various beverages, yeast can also produce alcohol, glycerin, mannitol, organic acids, vitamins, etc. Yeast can produce a large amount of thallus when cultured in an aerated manner, and its protein content can reach 50% of dry weight, which can be used for food and feed. In addition, the use of yeast fermentation to produce fuel ethanol and biodiese...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/04C12P7/22C12P7/24C12P17/06C12P7/40C12P7/62C12P5/02C12P5/00C12P7/26C12R1/645
CPCC12N1/16C12P5/002C12P5/007C12P7/04C12P7/22C12P7/24C12P7/26C12P7/40C12P7/62C12P17/06C12N1/145C12R2001/645
Inventor 何峰何晓罡刘守德
Owner 湖北同泽堂生物科技有限公司