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Method for extracting L-hydroxyproline from L-hydroxyproline fermentation liquor

A technology of hydroxyproline and fermentation broth, applied in the directions of additional food elements, animal feed, organic chemistry, etc., can solve problems such as large water consumption, environmental pollution, unstable yield, etc., to reduce the generation of waste water and avoid environmental problems. Pollution, saving effect of deionized water

Active Publication Date: 2016-07-20
TIANJIN JINGYE FINE CHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method has the following defects: ① Hydroxyproline is easily oxidized, and the yield is unstable; ② Crude hydroxyproline has many impurities and deep color, which affects the refined yield and product quality
③Large water consumption, large amount of acid and alkali, will produce a large amount of waste residue and waste water in the production process, causing very serious pollution to the environment
The refining production process that can overcome these defects, realize zero discharge of waste residue and wastewater in the extraction process, and completely eradicate environmental pollution in the production process of hydroxyproline has not been reported at home and abroad.

Method used

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  • Method for extracting L-hydroxyproline from L-hydroxyproline fermentation liquor

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Experimental program
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Effect test

Embodiment 1

[0028] Get 10L of microfiltration supernatant and microfiltration retentate (reserved for later use) through the fermentation liquid after L-hydroxyproline fermentation through 0.05 micron ~ 0.1 micron ceramic membrane, adjust pH to be 6 ~ 7, then with 12.5ml At a flow rate of / min, first pass through 1LC100 strongly acidic cation exchange resin, and then pass through 1.5LA830 weakly basic anion exchange resin. The ion exchange liquid is passed through a nanofiltration membrane with a molecular weight of 800-1000 Da to obtain a nanofiltration clear liquid and a nanofiltration retentate. The nanofiltration retentate is adjusted to a pH of 6-7 and sent back to the ion exchange resin circulation process. The nanofiltration liquid is added with 0.5% by volume of activated carbon for injection at 25° C. for 1.5 hours, and filtered to obtain a clear liquid with a light transmittance of 96%.

[0029] Concentrate the above decolorized solution in vacuum at 55°C to 1 / 8 of its volume, l...

Embodiment 2

[0034] Get the fermented liquid after L-hydroxyproline fermentation to obtain 99.6L microfiltration liquid and microfiltration retentate (reserved for later use) through 0.05 micron~0.1 micron ceramic membrane treatment, adjust pH to be 6~7, then use 135ml The flow rate of / min passes through 9L732 type strong acid cation exchange resin first, and then passes through 15LA830 weakly basic anion exchange resin. The ion exchange liquid is passed through a nanofiltration membrane with a molecular weight of 800-1000 Da to obtain a nanofiltration clear liquid and a nanofiltration retentate. The nanofiltration retentate is adjusted to a pH of 6-7 and sent back to the ion exchange resin circulation process. The nanofiltration liquid is decolorized at 25° C. for 1.5 hours by adding 0.5% of its volume of activated carbon for injection, and filtered to obtain a clear liquid with a light transmittance of 95.7%.

[0035] Concentrate the above decolorized solution in vacuum at 55°C to 1 / 8 o...

Embodiment 3

[0038] Feed Preparation of L-Hydroxyproline

[0039] A batch of reserve microfiltration retentate and 1 / 3 batch of mother liquor are mixed, spray-dried to obtain L-hydroxyproline feed product, and L-hydroxyproline is about 30%.

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Abstract

The invention discloses a method for extracting L-hydroxyproline from L-hydroxyproline fermentation liquor. The method comprises the following steps: performing microfiltration on L-hydroxyproline fermentation liquor, adjusting the pH value of a microfiltration solution to be neutral, then performing cationic and anionic resin adsorption, performing nanofiltration to obtain a nanofiltration clear solution, and performing primary concentration crystallization after decolorization to obtain a final product. According to the method, a primary mother solution is delivered back to the process of cationic and anionic resin adsorption for recycling, the mode of direct adsorption of amino acid of the conventional ion adsorption exchange process is changed into a mode of adsorption of impurities, and then primary crystallization is performed after nanofiltration impurity removal to obtain a product, so that the purity, content, yield and separation efficiency of the L-hydroxyproline can be greatly improved, the wastewater quantity is reduced, and the generated wastes are used for preparing feeds to avoid environmental pollution.

Description

technical field [0001] The invention relates to the technical field of amino acid extraction technology, in particular to a method for extracting L-hydroxyproline from L-hydroxyproline fermentation broth. Background technique [0002] Hydroxyproline (Hyp) is an imino acid, which is the product of L-proline hydroxylation, and its molecular formula is C 5 h 9 NO 3 . Hydroxyproline is white flaky crystal or crystalline powder, slightly sweet, with a melting point of 274°C, easily soluble in water and slightly soluble in ethanol. Hydroxyproline has 4 stereoisomers, and trans-4-hydroxyproline is more common in nature, and trans-4-hydroxyproline was first found in animal collagen. Trans-4-hydroxyproline is widely used in medicine, chemical industry, animal feed, nutrition and beauty industry, etc. [0003] At present, the isolation and extraction of hydroxyproline from the fermentation broth reported at home and abroad usually adopts the ion exchange method, and the fermentat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D207/16A23K20/142
CPCC07D207/16
Inventor 林俊辉赵体金彭久合
Owner TIANJIN JINGYE FINE CHEM
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