Unlock instant, AI-driven research and patent intelligence for your innovation.

A kind of grouper epinecidin-1 polypeptide and its application

A grouper and polypeptide technology, applied in the field of genetic engineering, can solve the problem of anti-fish virus that has not been reported.

Active Publication Date: 2019-06-25
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
View PDF1 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Grouper Epinecidin-1 is a small peptide encoding 67 amino acids. Synthetic Epinecidin-1 can inhibit Vibrio parahaemolyticus, Vibrio alginolyticus, V.vulnificus , Pasteurella multocida, Morganellamorganii, Aeromonas sobrio, Aeromonas hydrophila, Flavobacterium meningosepticum and Escherichia coli ) the role of DH5α, however, the role in anti-fish virus has not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of grouper epinecidin-1 polypeptide and its application
  • A kind of grouper epinecidin-1 polypeptide and its application
  • A kind of grouper epinecidin-1 polypeptide and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Artificial synthesis of grouper Epinecidin-1 polypeptide

[0027] After the amino acid sequence of grouper Epinecidin-1 (specifically shown in SEQ ID NO.1) is homologously compared with the amino acid sequence of Epinecidin-1 of other species, the mature peptide region of grouper Epinecidin-1 is determined, and then by The grouper Epinecidin-1 polypeptide is obtained by chemical synthesis, and its sequence is: FIFHIIKGLFHAGKMIHGLV (specifically shown in SEQ ID NO.3). Mass spectrometry was used to detect the mass of artificially synthesized grouper Epinecidin-1 polypeptide ( figure 1 ), after purification by HPLC high performance liquid chromatography ( figure 2 ). The correctly detected grouper Epinecidin-1 polypeptide was vacuum-dried into powder, divided into 10 mg per tube, and nitrogen was used as a protective gas to prevent oxidative denaturation of the synthetic polypeptide, and stored in an ultra-low temperature freezer at -80°C.

[0028] 2. Inhibitory eff...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an epinephelus Epinecidin-1 polypeptide and an application thereof. The amino acid sequence of the epinephelus Epinecidin-1 polypeptide is shown by SEQ ID NO.3. The epinephelus Epinecidin-1 polypeptide disclosed by the invention is an antibacterial peptide with antibacterial and antiviral activity and has the characteristic of fish virus resistance and can be used for preparing antiviral drugs; and the study of the epinephelus Epinecidin-1 polypeptide is of important scientific value and application value in further study and development of disease-resistant gene products.

Description

Technical field: [0001] The invention belongs to the field of genetic engineering, and in particular relates to a grouper Epinecidin-1 polypeptide and an application thereof. Background technique: [0002] Grouper (Epinephelus coioides) is an important economic species of marine aquaculture in southern my country, and its value is extremely high. In recent years, with the expansion of aquaculture scale, explosive deaths often occur, causing significant economic losses to the aquaculture industry. Virus diseases are common pathogens that endanger the healthy farming of grouper, mainly including grouper iridovirus (SGIV) and nerve necrosis virus (nervous necrosis virus, NNV). At present, antibiotics and chemical drugs are traditionally used in the prevention and control of fish diseases, which has caused serious problems such as drug residues, drug resistance and environmental pollution. For this reason, it has become a top priority to carry out basic research on fish immuni...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/46C12N15/12A61K38/17A61P31/14A61P31/20
CPCA61K38/00C07K14/461
Inventor 魏京广秦启伟黄友华
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI