A kit for evaluating sperm quality after in vitro capacitation and using method thereof

A kit and sperm technology, applied in the field of medical testing, can solve the problems of complex technical process, unscientific enzyme activity value, different enzyme activity value, etc., and achieve the effect of simple operation method and objective test results.

Active Publication Date: 2019-09-24
成都思瑞迪医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the method includes the expression detection of sperm sialidase NEU1 / 3, which requires a fluorescence microscope or flow cytometer, and has the following disadvantages: 1) The technical process is complicated; 2) It requires high skills and experience in experimental equipment and operators; 3 ) Not suitable for use in clinical laboratories and routine technicians; 4) Use abcam's kit, which is expensive
In addition, because the number of sperm in different samples is different, the value of expressed protein is different, and the corresponding enzyme activity value is also different. Therefore, it is not scientific to simply compare the enzyme activity values ​​​​of different samples.

Method used

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  • A kit for evaluating sperm quality after in vitro capacitation and using method thereof
  • A kit for evaluating sperm quality after in vitro capacitation and using method thereof

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Experimental program
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Effect test

Embodiment 1

[0042] A kit for assessing sperm quality after in vitro capacitation, said kit including detection buffer 1, BWW culture fluid, in vitro capacitation solution, fluorescent reagent, sialidase standard stock solution and detection buffer 2; The detection buffer 1 is a phosphate buffer; the detection buffer 2 is a phosphate buffer containing 0.05 mmol / l sodium acetate and 0.25 μg / μl bovine serum albumin.

[0043] The kit of the present invention respectively measures the sialidase activity of non-capacitated sperm and capacitated sperm (A is mouse, B is human), and the results show that the capacitated sperm can release sialidase into the capacitation fluid.

Embodiment 2

[0045] A kit for assessing sperm quality after in vitro capacitation, said kit including detection buffer 1, BWW culture fluid, in vitro capacitation solution, fluorescent reagent, sialidase standard stock solution and detection buffer 2; The detection buffer 1 is BWW culture medium; the detection buffer 2 is a phosphate buffer containing 0.05 mmol / l sodium acetate and 0.25 μg / μl bovine serum albumin.

[0046] The in vitro capacitation solution is HTF containing 5 mg / ml HSA.

Embodiment 3

[0048] A kit for assessing sperm quality after in vitro capacitation, said kit including detection buffer 1, BWW culture fluid, in vitro capacitation solution, fluorescent reagent, sialidase standard stock solution and detection buffer 2; The detection buffer 1 is a phosphate buffer; the detection buffer 2 is a phosphate buffer containing 0.05 mmol / l sodium acetate and 0.25 μg / μl bovine serum albumin.

[0049] The in vitro capacitation solution is HTF containing 5 mg / ml HSA.

[0050] The sialidase standard stock solution is Clostridium perfringens sialidase with a concentration of 5 U / ml.

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Abstract

Provided are a kit for evaluating the sperm quality after in vitro capacitation and a method of use thereof. The kit comprises a detection buffer 1, a BWW culture solution, a solution for in vitro capacitation, a fluorescent reagent, a sialidase standard stock solution and a detection buffer 2. The detection buffer 1 is a phosphate buffer or a BWW culture solution. The detection buffer 2 is a phosphate buffer containing 0.05 mmol / l of sodium acetate and 0.25 μg / μl of bovine serum albumin.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a kit for assessing sperm quality after in vitro capacitation and a use method thereof. Background technique [0002] With the continuous development of social industry, the total incidence of infertile couples increased significantly. In 2004, the statistics of the European Society of Reproduction: 25% of couples of childbearing age were unable to conceive within one year, 15% of them sought medical treatment, and 50% were infertility caused by male factors. The causes of male infertility are sexual dysfunction (1.7%), varicocele (12.3%), reproductive tract infection (6.6%), congenital dysplasia (2.1%) acquired disease (2.6%), Endocrine disorders (0.6%), immune factors (3.1%), and other abnormalities (3%), but up to 60% to 75% of patients cannot find the cause, which is called idiopathic male infertility, and they only show a few Abnormal sperm quality su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/34
Inventor 马芳马黔红潘倩马学
Owner 成都思瑞迪医疗科技有限公司
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