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Unimolecular recognition method based on split aptamer bi-metal nanoprobe

A nucleic acid aptamer and bimetallic nanotechnology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problem of limited observation time and achieve the effect of breaking through the poor specific recognition ability

Inactive Publication Date: 2016-07-20
DALIAN UNIV OF TECH
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Problems solved by technology

However, fluorescent substances also have shortcomings such as photobleaching or "blinking", which limit the observation time, so other alternative labeling substances are increasingly valued, and noble metal nanoparticles are one of them.

Method used

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  • Unimolecular recognition method based on split aptamer bi-metal nanoprobe

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Embodiment Construction

[0015] For example figure 1 As shown, the target target is a piece of single-stranded DNA, and its nucleic acid aptamer is a DNA molecule with a complementary base sequence to the target target; the nucleic acid aptamer is divided into two parts to obtain fragment 3 and fragment 4 respectively; Fragment 3 and fragment 4 are connected to gold nanoparticles to form a pair of molecular probes labeled with gold nanoparticles; the two probes thus obtained are injected into the sample to be tested in equal amounts, and the two split nucleic acid aptamer fragments are After the same target molecule is detected and bound back to it, a surface plasmon resonance coupling enhanced signal is generated in the gap between the two particles, which is used to specifically recognize the single-molecule target.

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Abstract

The invention belongs to the technical field of unimolecular imaging and recognition in biomedicine and medicine research. The method is characterized in that aptamer of a target is divided into two fragments, the split end of each fragment is marked with noble metal nanoparticles with a surface plasma resonance effect, so that aptamer molecular probes marked with two types of noble metal nanoparticles are formed; the two types probes are respectively bound to a same target molecule; during recovery, a generated stable surface plasma resonance coupling signal serves as specificity unimolecular recognition information of the target. The method is capable of achieving an effect of recognizing the target from noise background in which multiple single noble metal markers exist, has specificity target recognition capability, and is capable of solving the problem that currently used metal markers are poor in specificity recognition capability.

Description

technical field [0001] The invention belongs to the technical field of molecular imaging and identification in biomedical and medical research, relates to molecular imaging technology based on metal probes, in particular to a highly specific optical identification method based on metal nano probes. Background technique [0002] Imaging plays an important role in clinical diagnosis. Traditional imaging is based on pathological changes in the diagnosis of diseases, which are often found in the middle and late stages of the disease. Molecular imaging technology detects lesions at the molecular level, so as to truly achieve the goals of early detection, early diagnosis, and early intervention / treatment. The small size of the molecules requires the aid of markers to visualize them optically. Fluorescent substance is a widely used marker, which has high detection sensitivity and mature technology. However, fluorescent substances also have shortcomings such as photobleaching or "...

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Application Information

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IPC IPC(8): C12Q1/68
CPCC12Q1/6818C12Q2525/205C12Q2563/137C12Q2565/101
Inventor 洪昕
Owner DALIAN UNIV OF TECH
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