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A new target gene for diagnosis and treatment of tongue squamous cell carcinoma and its application

A tongue squamous cell carcinoma and gene technology, which is applied in the application field of KLK14 gene and its expression products in the diagnosis and treatment of tongue squamous cell carcinoma, can solve the problems of wide surgical resection, affecting the quality of life of patients, and large lesion area, and achieves a simplified quantitative detection method. Process, clear results, good repeatability

Active Publication Date: 2019-09-03
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the size of the lesion is large and the scope of surgical resection is wide, which will seriously affect the quality of life of patients after surgery.

Method used

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  • A new target gene for diagnosis and treatment of tongue squamous cell carcinoma and its application
  • A new target gene for diagnosis and treatment of tongue squamous cell carcinoma and its application
  • A new target gene for diagnosis and treatment of tongue squamous cell carcinoma and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The collection and preparation of embodiment 1 material

[0032] Tongue squamous cell carcinoma and paracancerous tissue samples were taken from patients with tongue squamous cell carcinoma undergoing radical resection, and normal mucosal tissues were taken from patients with trauma. The specimens were collected within 30 minutes of the surgical procedure, and then collected under the guidance of pathologists. The sample size of each case is about 1.0cm×l.0cm×l.0cm. The cancer tissue is the central area of ​​the tumor when the material is collected; the corresponding paracancerous tissue is the tissue 1-2cm away from the edge of the cancer tissue; the normal mucosa is excluding submucosal Epithelial tissue, the specimens were derived from surgical specimens of oral trauma. All specimens were placed in 1.5ml EP tubes and stored in liquid nitrogen tanks. All specimens were collected from patients who had not undergone any form of anti-tumor treatment and had no history ...

Embodiment 2

[0036] Embodiment 2 database and biological analysis method

[0037] RNA-seq read mapping

[0038] First, the low-quality reads were removed to obtain clean reads, and then TopHat v1.3.11 was used to match the clean reads with the UCSC H. sapiens reference genome (hg19), the human reference genome sequence (hgl9, updated on March 20, 2009) from Downloaded from UCSC database. The Hgl9 genome includes 25 reference sequences (reference assemblies) and 68 variable sequences (alternative assemblies). The pre-built index of H. sapiens UCSC hg19 version is downloaded from the TopHat homepage and used as a reference genome. When using TopHat to match the genome, each read (default to 20) is allowed to have multiple matching sites and a maximum of 2 mismatches . TopHat builds a library of possible splicing sites based on exon regions and GT-AG splicing signals, and maps reads that have not mapped to the genome to the genome based on these splicing site libraries. The system default...

Embodiment 3

[0046] Example 3 KLK14 gene expression in tongue squamous cell carcinoma tissue and adjacent tissue

[0047] 1. Materials and methods

[0048] 1. Materials

[0049] See Example 1 for the collection criteria. 36 cases of tongue squamous cell carcinoma tissues and adjacent tissues were collected and grouped and numbered.

[0050] 2. Method

[0051] 2.1 Extraction of RNA

[0052] (1) After taking out the sample from the liquid nitrogen tank, take 50-100mg (soy bean size) tissue and grind it, then add 1ml Trizol reagent. Transfer the Trizol-tissue lysis mixture to a 1.5ml centrifuge tube, and then extract the total RNA according to the standard operation of the Trizol reagent;

[0053] (2) In the separation stage, add 0.2ml chloroform to every 1ml Trizol, shake the test tube vigorously for 10 seconds, mix well, and let stand at room temperature for 5 minutes. Then centrifuge at 12000rmp for 15 minutes;

[0054] (3) Precipitation of RNA Aspirate the upper layer of water-like ...

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Abstract

The invention provides a novel target gene for diagnosing and treating tongue squamous carcinoma and application thereof and particularly relates to application of a KLK14 gene and an expression product thereof to diagnosis and treatment of tongue squamous carcinoma. To research the occurrence and development mechanisms of tongue squamous carcinoma, search for an effective molecular target gene for diagnosing and treating tongue squamous carcinoma, promote early diagnosis, prevention and treatment of the disease and lower the death rate of tongue cancer, firstly, RNA-seq sequencing is utilized to detect differential expression genes of tongue squamous carcinoma, cancer branch and normal oral mucosa; secondly, a Real-time PCR technology is utilized to verify the sequencing result; then, an interference technology is utilized, and expression of the candidate gene KLK14 in tongue squamous carcinoma cells SCC15 is silenced. By means of the novel target gene for diagnosing and treating tongue squamous carcinoma and application thereof, an experimental foundation is laid for clinical application of the KLK14 gene to tongue squamous carcinoma, and a new target gene and theoretical basis are provided for early diagnosis and treatment of tongue squamous carcinoma.

Description

technical field [0001] The present invention relates to the field of gene diagnosis and treatment, in particular to a new target gene for diagnosis and treatment of tongue squamous cell carcinoma and its application, more specifically to the application of KLK14 gene and its expression product in diagnosis and treatment of tongue squamous cell carcinoma. Background technique [0002] Tongue squamous cell carcinoma (TSCC) occupies the first place in the incidence of oral squamous cell carcinoma. The occurrence of tongue squamous cell carcinoma is a multi-factor, multi-step and multi-stage complex process. The etiology is also complex and diverse, involving physical and chemical factors, microbial infection, genetic , race, etc. Although the etiology and related mechanisms of tongue squamous cell carcinoma have been the focus of research and some achievements have been made, tongue squamous cell carcinoma still has a high mortality rate and poor prognosis. The reason for the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/11G01N33/68G01N33/574A61K48/00A61K31/713A61P35/00
Inventor 张海霞胡春宏刘先领何燕邓超冯也倩
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
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