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A detection method for prostate specific antigen

A prostate-specific and detection method technology, applied in the field of prostate-specific antigen detection, can solve problems such as low precision, and achieve the effects of saving costs and improving detection sensitivity

Active Publication Date: 2017-11-21
NANCHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The present invention aims at the technical defect of prior art, provides a kind of detection method for prostate specific antigen, to solve the low precision of detection method of prostate specific antigen ELISA of prior art

Method used

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  • A detection method for prostate specific antigen
  • A detection method for prostate specific antigen
  • A detection method for prostate specific antigen

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] Example 1 (Application of the novel fluorescent ELISA detection method for the detection of PSA in the present invention in the detection of PSA content in serum)

[0051] When the novel fluorescent ELISA detection method of the present invention is used to detect the content of prostate specific antigen in serum, it is implemented through the following steps: sample pretreatment, detection by the detection method of the present invention, and analysis of the results.

[0052] (1) Sample pretreatment

[0053] (1) Dilute the purchased PSA standard with whole serum, and the concentration is determined according to the actual detection limit; (2) The diluted PSA whole serum sample is placed in a 4°C refrigerator for later use. (2) detect the prostate specific antigen content in the above-mentioned sample with detection method of the present invention

[0054] Take the microtiter plate coated with polyclonal antibody against prostate specific antigen content, add standard ...

Embodiment 2

[0066] Embodiment 2 (using horseradish peroxidase as antibody labeling enzyme, using TMB as the prostate specific antigen ELISA detection method of chromogenic substrate)

[0067] When the traditional ELISA detection method is used to detect the content of prostate specific antigen in serum, it is implemented through the following steps: sample pretreatment, detection by traditional ELISA detection method, and analysis of the results.

[0068] (1) Sample pretreatment

[0069] (1) Dilute the purchased PSA standard with whole serum, and the concentration is determined according to the actual detection limit; (2) The diluted PSA whole serum sample is placed in a 4°C refrigerator for later use. ((2) Use the traditional ELISA detection method to detect the content of prostate specific antigen in the above samples

[0070] Take the microtiter plate coated with polyclonal antibody against prostate specific antigen content, add standard substance / sample 100 μL / well to the correspondi...

Embodiment 3

[0076] The invention relates to a detection method for prostate specific antigen, which belongs to the double-antibody sandwich ELISA method. The method is for detection of prostate specific antigen, and the enzyme used for labeling antibody in the method is catalase C100.

[0077] On the basis of the above technical solutions, the following conditions are met:

[0078] The substrate in the method includes hydrogen peroxide and mercaptopropionic acid modified cadmium telluride quantum dots.

[0079] The specific detection method includes the following steps:

[0080] 1) coated with prostate specific antigen antibody;

[0081] 2) Take the coated antibody in step 1), mix it with the sample to be tested, react in a light-proof environment at 35°C for 40 minutes, and wash;

[0082] 3) Then add biotinylated PSA monoclonal antibody to mix, react at 35°C in a dark environment for 40min, and wash;

[0083] 4) Then add streptavidin-labeled catalase C100 to mix, react at 35°C in a da...

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Abstract

The invention provides a method for detecting prostate specific antigens. The method includes binding the prostate specific antigens with coated polyclonal antibodies; connecting the prostate specific antigens with biotinylated monoclonal antibodies; connecting the prostate specific antigens with streptavidine-labeled catalase C100. The method has the advantages that hydrogen peroxide can be decomposed under the catalytic effects of the catalase, accordingly, fluorescence quenching of mercaptopropionic acid modified cadmium telluride quantum dots can be reduced, and the concentration of the prostate specific antigens in samples can be judged according to the fluorescence intensity; the method is based on double-antibody sandwich enzyme-linked immunological technologies, and biotin-avidin systems are used for reaction amplification; more importantly, novel antibody-labeled enzymes (the catalase C100) and sensitive fluorescence substrates (the cadmium telluride quantum dots) are adopted and are matched with effective reaction conditions, accordingly, the detection sensitivity can be obviously enhanced, the cost can be lowered, the detection efficiency can be enhanced, and the method has an excellent popularization prospect.

Description

technical field [0001] The invention relates to the technical field of antigen detection, and further relates to an ELISA-based antigen detection technology, in particular to a detection method for prostate specific antigen. Background technique [0002] In recent years, with the improvement of people's living standards, changes in dietary structure, and aggravation of environmental pollution, the incidence of prostate cancer has been increasing year by year. Prostate cancer has surpassed skin cancer to become the most common cancer in North American men and the second leading cause of cancer death. Prostate specific antigen (PSA) is a specific glycoprotein isolated and purified from prostate tissue, which has serine protease activity. PSA is currently recognized as the most valuable marker of prostate cancer. It is an indispensable and preferred TM detection method for the diagnosis of prostate cancer with high specificity and strong sensitivity in clinical applications. ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/58G01N33/577G01N33/574G01N33/573
CPCG01N33/573G01N33/57434G01N33/581G01N33/588
Inventor 熊勇华黄小林许恒毅陈锐梁毅湛胜楠裴可熊颖段宏郑玲燕周耀峰
Owner NANCHANG UNIV