Preparation method of egg-chicken feed additive
A production method and technology for laying hen feed are applied in the field of production of laying hen feed additives, which can solve problems such as affecting human health, residues, toxic and side effects of chemical drugs, etc., and achieve the effects of preventing and treating heat stress disease of laying hens and improving quality.
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[0011] specific implementation plan
[0012] The bacterial strain Bacillus subtilis CICC10071 used in the present invention was purchased from China Industrial Microorganism Culture Collection and Management Center.
[0013] Weigh 10 g of peptone, 10 g of beef extract, 10 g of glucose, and 5 g of sodium chloride, and add water to the volume to 1000 ml to obtain the Bacillus subtilis seed liquid culture medium. Take 100ml of seed solution and culture it in a 500ml Erlenmeyer flask, sterilize at 121°C for 20 minutes, cool to 30°C, inoculate Bacillus subtilis, and cultivate on a shaker at 160rpm and 30°C for 22 hours to obtain Bacillus subtilis seed solution.
[0014] Crush peanut meal and pass through a 40-mesh sieve to obtain peanut meal powder. Weigh a certain mass of peanut meal powder, add 20 times the mass of 1% glucose solution, stir evenly, sterilize at 121°C for 20 minutes, and cool down to 30°C. ℃, inoculate Bacillus subtilis seed solution with 8% inoculum amount, ferm...
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