Grass carp bacterial septicemia and grass carp bacterial red skin disease bigeminy propolis inactivated vaccine and preparing technology

Abstract

The invention discloses a grass carp bacterial septicemia and red skin disease bigeminy propolis inactivated vaccine.The grass carp bacterial septicemia and red skin disease bigeminy propolis inactivated vaccine is prepared from an aeromonas hydrophila culture inactivating solution, a pseudomonas aeruginosa culture inactivating solution, normal saline and a propolis ethyl alcohol soaking solution.The invention further provides a factory-like preparing method for the grass carp bacterial septicemia and red skin disease bigeminy propolis inactivated vaccine.The factory-like preparing method comprises the steps that the aeromonas hydrophila culture inactivating solution, the pseudomonas aeruginosa culture inactivating solution, the normal saline and the propolis ethyl alcohol soaking solution are mixed evenly at the proportion of 12:10:76.4:1.6 and stirred, sterile filling is carried out, and the grass carp bacterial septicemia and red skin disease bigeminy propolis inactivated vaccine finished product is obtained.According to the grass carp bacterial septicemia and red skin disease bigeminy propolis inactivated vaccine and the factory-like preparing method, the defect that a grass carp single inactivated vaccine is only used for one kind of grass carp pathogenic bacteria is overcome, the bigeminy vaccine is provided to reach the effect of simultaneously defending two kinds of pathogenic bacteria of grass carps through one-time immunization, and the vaccine is convenient to apply through injection and soaking.

Description

technical field [0001] The invention belongs to the field of protein vaccines, and in particular relates to a dual propolis inactivated vaccine for grass carp bacterial sepsis and grass carp bacterial red skin disease and a preparation process. Background technique [0002] The pathogen of grass carp bacterial septicemia is Aeromonas hydrophila, which is a common and serious infectious disease of grass carp. The incidence of sepsis in mature fish ponds is above 46%, and that in fingerling ponds is above 25%. Among them, the incidence of intensive breeding and high-yield ponds is the highest, reaching more than 60%. The diseased grass carp is about 3-3% from the onset of symptoms to death. 10d, often causing huge economic losses. Bacterial red skin disease of grass carp can occur throughout the year. It is one of the main diseases of grass carp fingerlings and adult fish, especially for 1-year-old grass carp. The rate can reach 67%, and the mortality rate is as high as 16%....

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Problems solved by technology

[0003] The purpose of the present invention is to overcome the shortcoming that the grass carp single inactivated vaccine is only aimed at one kind of grass carp pathogenic bacteria, and provide a double vaccine to achieve the effect that one immunization can resist two grass carp pathogenic bacteria at the same time, and the vaccine is conveniently administered by injection and soaking

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