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Method for extracting micromolecular intracellular metabolite from nori

A technology for small molecules and metabolites, which is applied in the field of extraction of intracellular metabolites of small molecules, can solve problems such as environmental pollution and waste, and achieve the effects of high yield, time saving and fast separation speed

Active Publication Date: 2016-08-03
南通中国科学院海洋研究所海洋科学与技术研究发展中心 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Disposal of these inferior laver as garbage not only causes huge waste, but also causes environmental pollution

Method used

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  • Method for extracting micromolecular intracellular metabolite from nori
  • Method for extracting micromolecular intracellular metabolite from nori

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Extract free amino acid in the laver of embodiment 1

[0034] 1. Absorb the water on the surface of fresh inferior laver with absorbent paper, freeze it in liquid nitrogen and store it at -80°C for later use;

[0035] 2. Take an appropriate amount of seaweed stored at -80°C, grind it into powder with liquid nitrogen, and weigh 50mg of the material with an analytical balance into a 1.5ml EP tube;

[0036] 3. Add 1ml of 100% by volume methanol for extraction, extract at -20°C, and shake the EP tube during the extraction to make the extraction more complete;

[0037] 4. Centrifuge at 10,000g for 2 minutes at 4°C, take the supernatant and add water and chloroform (volume ratio of methanol, water, and chloroform is 1:1:1), mix well, and let stand for 5 minutes; then centrifuge at 10,000g for 2 minutes at room temperature, Take the aqueous phase by centrifugation to obtain free amino acids;

[0038] 5. Take an appropriate amount of the free amino acid sample obtained in ste...

Embodiment 2

[0041] Extract free monosaccharide from laver in embodiment 2

[0042] 1. Absorb the water on the surface of fresh inferior laver with absorbent paper, freeze it in liquid nitrogen and store it at -80°C for later use;

[0043] 2. Take an appropriate amount of seaweed stored at -80°C, grind it into powder with liquid nitrogen, and weigh 100mg of the material with an analytical balance into a 1.5ml EP tube;

[0044] 3. Add 1ml of 100% by volume methanol for extraction, extract at -20°C, and shake the EP tube during the extraction to make the extraction more complete;

[0045] 4. Centrifuge at 10,000g for 2 minutes at 4°C, take the supernatant, extract the precipitate twice with 1ml of 50% by volume methanol at -20°C, add 1ml of water and extract once at 4°C, and extract for 5 minutes each time. Finally, all supernatants were combined;

[0046] 5. Take 2ml of combined supernatant, add 1ml of chloroform to extract, mix well, let stand for 5 minutes, centrifuge at 10000g for 2 mi...

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Abstract

The invention discloses a method for extracting micromolecular intracellular metabolite from nori. The method includes the following steps that 1, nori is ground into powder with liquid nitrogen; 2, the powder is added into an extracting solution for extraction, and supernatant liquor is obtained; the supernatant liquor is extracted, a water phase is taken, and the micromolecular intracellular metabolite is obtained. By means of the method, the micromolecular intracellular metabolite can be extracted from nori, and the micromolecular intracellular metabolite is small in pigment pollution, high in yield and capable of serving as a reference for extracting active matter from nori; besides, the extracting method is simple and easy to implement.

Description

technical field [0001] The invention relates to a method for extracting small-molecule intracellular metabolites, in particular to a method for extracting small-molecule intracellular metabolites from laver. Background technique [0002] Laver has important economic value and is one of the main economic seaweeds in my country. There are two main varieties of laver cultivated in my country, that is, altar laver in the south and laver zebra in the north. Altar laver is a unique species in my country and is mainly cultivated in the south of the Yangtze River (Fujian Province and Guangdong Province). Porphyra zebra is widely distributed, except in northern my country (Jiangsu Province and Shandong Province, mainly in Nantong, Yancheng and Lianyungang sea areas), it is cultivated in Korea and Japan, and has important economic value. [0003] Seaweed is rich in phycobiliprotein, essential amino acids, polysaccharides, iodine, phosphorus, calcium and other active substances. It h...

Claims

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Application Information

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IPC IPC(8): B01D11/02C07B63/00C07H1/08C07C227/40
CPCB01D11/02C07B63/00C07C227/40C07H1/08
Inventor 王广策黄爱优
Owner 南通中国科学院海洋研究所海洋科学与技术研究发展中心
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