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Preparation of Mesoporous Molecularly Imprinted Fluorescent Probe and Its Detection of Tetracycline Antibiotics

A molecular imprinting, fluorescent probe technology, applied in fluorescence/phosphorescence, analytical materials, luminescent materials, etc., can solve the problems of cumbersome sample pretreatment process, poor target molecule selectivity, small specific surface area, etc., to reduce analysis costs, select Strong and selective effect

Inactive Publication Date: 2018-07-31
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problems of cumbersome sample pretreatment process, long analysis time and high cost in existing methods for detecting tetracycline antibiotic residues, and the selection of target molecules by existing molecularly imprinted polymer-quantum dot composite materials Poor performance, small specific surface area and low adsorption capacity

Method used

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  • Preparation of Mesoporous Molecularly Imprinted Fluorescent Probe and Its Detection of Tetracycline Antibiotics
  • Preparation of Mesoporous Molecularly Imprinted Fluorescent Probe and Its Detection of Tetracycline Antibiotics
  • Preparation of Mesoporous Molecularly Imprinted Fluorescent Probe and Its Detection of Tetracycline Antibiotics

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Embodiment 1

[0018] This embodiment is the preparation of mesoporous molecularly imprinted fluorescent probe, which is specifically completed according to the following steps:

[0019] 1. Preparation of molecularly imprinted polymer: Mix 1.5g of aureomycin solid with 30mL of tetrahydrofuran and 6mL of isocyanatopropyltriethoxysilane, stir and react at 100°C for 24h (nitrogen protection), and the stirring speed is 350r / min, and then remove THF by a rotary evaporator to obtain a prepolymerization solution; then add 0.05mL prepolymerization solution, 0.45g hexadecyltrimethylammonium bromide, 2.25mL ethyl orthosilicate, 67.5mL volume fraction Add 56% ethanol aqueous solution into the Erlenmeyer flask, finally add 0.875mL 2mol / L NaOH solution, stir at room temperature for 2 hours, then put it in a high-temperature reactor and react at 120°C for 48 hours, and perform suction filtration after the reaction to obtain a solid product. The solid product was centrifugally washed with distilled water ...

Embodiment 2

[0023] This embodiment is an application of a mesoporous molecularly imprinted fluorescent probe in the detection of chlortetracycline, which is completed according to the following steps: respectively mix 0.2 g of mesoporous molecularly imprinted fluorescent probe with a concentration of 50-1000 ng / mL Mix the chlortetracycline sample solution and adjust the pH of the mixture to 8, disperse it evenly by ultrasonication for 2 minutes, and then use the fluorescence spectrophotometer to perform fluorescence analysis in turn after standing at room temperature for 10 minutes, that is, the detection of chlortetracycline by the mesoporous molecular imprinted fluorescent probe is completed. The application in the element; its fluorescence detection condition is set as follows:

[0024]

[0025] The results show that the standard addition recovery rate of aureomycin obtained by the method established by the present invention is 92.1%~97.5%, the relative standard deviation (RSD) is 2....

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Abstract

The invention discloses preparation of a mesoporous molecular imprinting fluorescent probe and detection of tetracycline antibiotics by the mesoporous molecular imprinting fluorescent probe, relates to a method for detecting tetracycline antibiotic residues, and aims to solve the problems of tedious sample preprocessing process, long analysis time and high cost of an existing method for detecting tetracycline antibiotic residues as well as the problems of poor target molecule selectivity, relatively small specific surface area and low adsorption amount of an existing molecular imprinting polymer-quantum dot composite material. The preparation method comprises the following steps: 1, preparing a molecular imprinting polymer; 2, preparing a Mn doped ZnS quantum dot; and 3, preparing the mesoporous molecular imprinting fluorescent probe. A use method comprises the following steps: adding the mesoporous molecular imprinting fluorescent probe into a sample solution containing the tetracycline antibiotics, performing ultrasonic treatment for uniform dispersion, and performing fluorescent detection. The mesoporous molecular imprinting fluorescent probe prepared with the method is high in selectivity and high in sensitivity.

Description

technical field [0001] The invention relates to a method for detecting residues of tetracycline antibiotics. Background technique [0002] Tetracycline antibiotics are the most commonly used antibiotics. In addition to treating their own diseases, they are also widely used in animal breeding and aquaculture. However, the excessive abuse of antibiotics has seriously threatened people's health. At present, the methods commonly used in my country to detect antibiotics include liquid chromatography or liquid chromatography and mass spectrometry. High cost and long analysis time. Liquid chromatography and mass spectrometry have very high requirements on sensitivity, reproducibility, and selectivity. The sample pretreatment process is cumbersome, labor time is long, and analysis cost is high. Therefore, it is very important for human health and ecological civilization to develop an efficient, fast and low-cost method for detecting tetracycline antibiotic residues. [0003] Molec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08L83/08C08K3/30C08K3/08C08K5/548C08J9/26C08G77/26G01N21/64C09K11/57C09K11/02
CPCC08J9/26C08J2383/08C08K3/08C08K3/30C08K5/548C08K2003/3036C09K11/025C09K11/574G01N21/643G01N2021/6432C08L83/08
Inventor 陈立钢张靓贾丽娜
Owner NORTHEAST FORESTRY UNIVERSITY
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