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Nucleic acid label and kit for auxiliary diagnosis of Kawasaki disease

A nucleic acid labeling, auxiliary diagnosis technology, applied in subjects of intervention, biomarkers, calcineurin 3 catalytic subunit gamma isoform protein gene, protein, kit for auxiliary diagnosis of Kawasaki disease, detection of KD biological Markers, drugs for the treatment of Kawasaki disease, 25% of KD children treated can develop permanent coronary artery damage, or its accelerator field, to predict whether there is concurrent coronary artery damage, stable results, and great clinical application value.

Inactive Publication Date: 2016-08-03
SOOCHOW UNIV AFFILIATED CHILDRENS HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As the most common acquired cardiovascular disease in children, Kawasaki disease manifests as systemic immune small and medium-sized vasculitis and vascular endothelial dysfunction. However, the expression of miRNA in plasma and its mechanism of action are rarely reported at home and abroad.

Method used

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  • Nucleic acid label and kit for auxiliary diagnosis of Kawasaki disease
  • Nucleic acid label and kit for auxiliary diagnosis of Kawasaki disease
  • Nucleic acid label and kit for auxiliary diagnosis of Kawasaki disease

Examples

Experimental program
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Effect test

Embodiment 1

[0038] Example 1: 6 children with KD in the acute stage and 6 healthy children who visited the Children's Hospital Affiliated to Soochow University were selected as controls. Fasting blood was drawn in the morning, plasma was separated, and total RNA in the plasma was extracted, and miRNA chip hybridization technology was used to detect Kawasaki disease Differentially expressed miRNAs in the plasma of the control group and the normal control group. Eight children with acute KD and 8 healthy children were selected, and real-time quantitative PCR (real-time quantitative PCR, RT-qPCR) technology was used to verify the chip results. Three databases, TargetScan, PITA and microRNAorg, were used to predict target genes of differential miRNAs, and DAVID bioinformatics analysis software was used to perform GO and KEGG analysis on common target genes to find target genes related to KD vascular inflammation.

[0039] 1. The instrument involved in the embodiment

[0040] 1.1 Main instrum...

Embodiment 2

[0141] Example 2, taking hsa-miR-223-3p as the research object, to explore the expression of hsa-miR-223-3p in plasma before and after KD gamma globulin treatment and its correlation with the inflammatory cytokine IL-6. To explore the clinical significance of hsa-miR-223-3p detection in KD. Thirty children with KD were selected, including 10 patients with coronary artery injury and 20 patients with non-coronary artery injury. There were 12 cases of fever and normal control group. RT-qPCR was used to detect the expression of hsa-miR-223-3p in the acute phase and subacute phase of KD, and the level of IL-6 in plasma was detected by enzyme-linked immunosorbent assay (ELISA). Differences between groups were analyzed, and the correlation between hsa-miR-223-3p and IL-6 was analyzed.

[0142] 1. Instruments involved in the embodiment

[0143]

[0144]

[0145] Analysis software: AscentsoftwareforMultiskan

[0146] 2. Main reagents involved in the examples

[0147] RT-qPCR...

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Abstract

The invention belongs to disease detection kit, and concretely relates to a kit for auxiliary diagnosis of Kawasaki disease by using miRNA as a biological label. The nucleic acid label is hsa-miR-223-3p, and the kit includes a primer subjected to a real-time quantitative polymerase chain reaction (RT-qPCR) for hsa-miR-223-3p. The kit can combine blood routine examination, high-sensitivity C reactive protein, erythrocyte sedimentation rate and cardiac ultrasonography, and is conducive to auxiliary diagnosis of Kawasaki disease and prediction damage of coronary artery.

Description

[0001] The invention belongs to a disease detection kit, in particular to a kit for assisting diagnosis of Kawasaki disease using miRNA as a biological marker. Background technique [0002] Kawasaki disease (Kawasaki disease, KD) is an acute vasculitic syndrome, mostly in infants and young children, and is the most common acquired heart disease in children. Cancer is one of the risk factors for ischemic heart disease in adulthood. The etiology and pathogenesis of KD are still not very clear. The current research trend believes that KD is triggered by exogenous antigens on the basis of a certain genetic susceptibility, causing immune dysfunction, high activation of the immune system and vasculitis. It is the basic pathological feature of KD. There are currently no specific laboratory indicators for the diagnosis of KD, and the diagnosis is mainly based on clinical manifestations. Although echocardiography plays an important role in the diagnosis of KD, more sensitive and speci...

Claims

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Application Information

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IPC IPC(8): C12Q1/68C12N15/113
CPCC12Q1/6883C12Q2600/136C12Q2600/158C12Q2600/178
Inventor 陈烨吕海涛
Owner SOOCHOW UNIV AFFILIATED CHILDRENS HOSPITAL
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