Method for treating pesticide residues and heavy metal mercury in sewage
A technology for pesticide residues and heavy metals, applied in chemical instruments and methods, biochemical equipment and methods, filtration treatment, etc., can solve problems such as secondary pollution of soil and water sources, and achieve improved treatment effects, good application prospects, and high removal efficiency. Effect
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Embodiment 1
[0026] The treatment of embodiment 1 mercury and pesticide residual sewage
[0027] When the sewage enters the water, the concentration of mercury is 30mg / L, dimethoate 20mg / L, fenitrothion 20mg / L, methomyl 20mg / L.
[0028] Pretreatment: remove the suspended solids in the treated sewage by filtration and sedimentation;
[0029] Main treatment: adding the bacterium Pseudomonas putida SPl CGMCC No. 3887 disclosed in CN 102373161A cultivated to the logarithmic growth phase into the sewage, and under normal temperature conditions, flow culture for 24 hours, and then the sewage can be removed HG;
[0030] Post-treatment: the sewage is treated overnight through the zeolite and activated carbon adsorption pool again to adsorb pesticide residues;
[0031] After the treated sewage is disinfected, it is directly discharged or reused. After testing, the concentration of mercury was 2.2mg / L, dimethoate 15.4mg / L, fenitrothion 14.3mg / L, and methomyl 16.4mg / L.
Embodiment 2
[0032] Embodiment 2 Transformation and acquisition of degradable pesticide gene
[0033] According to the method disclosed in CN1970741A, CYP9G2 and POR genes were cloned. The POR gene was modified as follows, and the corresponding Asp44Ser (indicating that the 44th Asp was mutated into Ser), Gln74Tyr, Leu96Lys, Asp137Ala, His199Gly, Ile231Gly, Asp296Glu, Gly316Asn, Asp346Glu, Pro381Cys, Tyr484Lys, Ser519Arg, Hisl by multiplex PCR , the mutation sites were respectively introduced into the protein shown in GenBank: L19897, thereby constructing different mutant POR genes (can be obtained by referring to the preparation method of the prior art). The mutated POR gene and CYP9G2 gene were respectively connected to the expression vector according to the method for preparing CN1970741A, and the successfully verified recombinant plasmid was transformed into the Pseudomonas putida SP1 CGMCC No. 3887 strain, which was successfully constructed by PCR detection.
Embodiment 3
[0034] Example 3 Verification of Sewage Degradation Effect
[0035] The strains containing different mutant proteins were respectively followed the method of Example 1 to verify the treatment effect on sewage. When the sewage enters the water, the concentration of mercury is 30mg / L, dimethoate 20mg / L, fenitrothion 20mg / L, methomyl 20mg / L. The strains and bacteria transformed with unmutated CYP9G2 and POR genes were used as controls. The specific results are as follows:
[0036]
[0037]
[0038] From the above results, it can be seen that the bacteria introduced with specific mutations of POR and CYP9G2 gene mutations have enhanced mercury and pesticide removal effects, which fully demonstrates that not all changes have better effects. The applicant obtained several sites with better effects through a large number of verifications.
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