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Pigeon pea endophytic fungi high in yield of flavipin and application of pigeon pea endophytic fungi

A technology of endophytic fungus and aspergillus, applied in the field of bioengineering, can solve the problems of destroying ecological balance, low efficiency of disease control, and lack of standard operation technology

Pending Publication Date: 2016-08-10
NORTHEAST FORESTRY UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there are mainly two methods for the prevention and control of these plant diseases: one is through the management of agricultural prevention and control measures, such as reasonable crop rotation, good cultivation, timely sowing and sowing depth, and strengthening field management, etc., which can play a certain role in the prevention and control of diseases , but this kind of method is labor-intensive and time-consuming, the operation technology is lack of standardization, the controllability is poor, and the control efficiency of the disease is not high; the other is through the large and frequent use of pesticides. Pathogens are easy to develop drug resistance, and the extensive use of pesticides will cause great harm to human health and the ecological environment, and at the same time seriously damage the ecological balance

Method used

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  • Pigeon pea endophytic fungi high in yield of flavipin and application of pigeon pea endophytic fungi
  • Pigeon pea endophytic fungi high in yield of flavipin and application of pigeon pea endophytic fungi
  • Pigeon pea endophytic fungi high in yield of flavipin and application of pigeon pea endophytic fungi

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specific Embodiment approach 1

[0019] In this embodiment, the pigeonpea endophytic fungus with high yield of aflatoxin is Chaetomium globosum (Chaetomiumglobosum) ELP-8, which has been preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC No: M 2015001, The deposit address is Wuhan University, Wuhan, and the deposit date is January 5, 2015.

[0020] In the present embodiment, Chaetomium globosum (Chaetomium globosum) ELP-8 has bacterial colony growth after being cultivated on PDA solid medium for 3 days, and the bacterial colony starts to be white and thin flocculent, and the colony diameter reaches 8cm after about two weeks of growth. The surface of the colony is gray-brown and a large number of small black particles begin to appear, and the back is amber.

[0021] In the present embodiment, Chaetomium globosum (Chaetomium globosum) ELP-8 is isolated from healthy pigeonpea leaves, and the pigeonpea leaves are collected from the botanical garden of Northeast Forestr...

specific Embodiment approach 2

[0030] In the present embodiment, Chaetomium globosum (Chaetomium globosum) ELP-8, the aflatoxin in this bacterial strain is measured as follows to the potted control effect of crop root rot:

[0031] Put 100g of sorghum grains into a 250mL Erlenmeyer flask, soak in water for 12h, filter to dry, and sterilize under high pressure. Inoculate 5 blocks of 5mm activated Fusarium oxysporum into each bottle, cultivate at 28°C for 5 days, and make dry powder for later use.

[0032] The soil was taken from the botanical garden of Northeast Forestry University, and the soil was sterilized by autoclaving (121°C) twice, each time for 2 hours, and 1 day apart.

[0033] After thoroughly mixing 10 g of Fusarium oxysporum powder and 250 g of sterilized soil, put it into a cultivation pot (outer diameter 6.5 cm, height 8 cm). Sow the soybean, wheat, and corn seeds sterilized on the surface in the cultivation pots, 10 seeds in each cultivation pot, and treat 3 nutrition pots in each group. Ca...

specific Embodiment approach 3

[0047] In the present embodiment, Chaetomium globosum (Chaetomium globosum) ELP-8, the aflatoxin in this bacterial strain is measured as follows to the potted control effect of vegetable fusarium wilt:

[0048] Put 100g of sorghum grains into a 250mL Erlenmeyer flask, soak in water for 12h, filter to dry, and sterilize under high pressure. Each bottle was inoculated with 5 pieces of 5mm activated Fusarium wilt, cultured at 28°C for 5 days, and made into dry powder for later use.

[0049] The soil was taken from the botanical garden of Northeast Forestry University, and the soil was sterilized by autoclaving (121°C) twice, each time for 2 hours, and 1 day apart.

[0050] After fully mixing 10 g of Fusarium wilt powder and 250 g of sterilized soil, put it into a cultivation pot (outer diameter 6.5 cm, height 8 cm). Sow the surface-sterilized cucumber, capsicum, and eggplant seeds in the cultivation pots, with 10 seeds in each cultivation pot, and each group handles 3 nutrition ...

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Abstract

Disclosed are pigeon pea endophytic fungi high in the yield of flavipin and an application of the pigeon pea endophytic fungi to prevent and treat plant diseases. The endophytic fungi are characterized by being chaetomium globosum (ELP-8), having been preserved at the China Center for Type Culture Collection, and having the preservation number being CCTCC No: M 2015001. The preservation address is Wuhan University at Wuhan city, and the preservation date is Jan. 5, 2015. Flavipin produced in a fermentation liquid of the pigeon pea endophytic fungi ELP-8 has an excellent inhibition effect on fusarium pathogenic fungus. The endophytic fungi have a biological prevention and treatment effect on plant diseases, caused by fusarium, of crops, vegetables, and forests, and provide a novel path and method for researching microbial pesticides.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a plant endophytic fungus isolated from pigeonpea, which has a biological control effect on plant diseases such as crops, vegetables and forest trees caused by Fusarium infection . Background technique [0002] Pigeon pea [Cajanus cajan (L.) Millsp.], English name Pigeon pea, alias pigeon pea, bean paste tree, clover bean, tree bean, red bean, millennium bean, Rong bean, etc., because its pods are knotted on the branches of the crown On, it is called pigeonpea. Pigeon beans belong to the evergreen shrubs of Fabaceae and Papilionaceae, and are annual or perennial woody plants of the pigeonpea genus. In the pigeonpea genus, 32 species have been found, and pigeonpea is the only cultivated species, and it is the only edible woody and most versatile legume crop in the world so far. Its roots, stems, leaves, flowers, and pods are all treasures, with extremely high comprehensi...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/645
Inventor 付玉杰姚美玲高畅孙姣王微赵春建顾成波
Owner NORTHEAST FORESTRY UNIVERSITY
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