Procalcitonin detection kit, and method of measuring content of procalcitonin therewith

A technology of procalcitonin and kits, which is applied in the field of medical testing, can solve the problems of long sample preparation time, labor and time consumption, etc., and achieve the effects of high accuracy, good precision, and improved detection sensitivity

Inactive Publication Date: 2016-08-10
SHANDONG SHENGBAILING MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When the sample size is large, the time to prepare the sample is longer than the test time, which consumes manpower and time

Method used

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  • Procalcitonin detection kit, and method of measuring content of procalcitonin therewith
  • Procalcitonin detection kit, and method of measuring content of procalcitonin therewith
  • Procalcitonin detection kit, and method of measuring content of procalcitonin therewith

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specific Embodiment approach

[0035] The specific embodiment of the present invention is described by the following examples:

example 1

[0037] Preparation of procalcitonin assay kit

[0038] Preparation method of reagent 1 (R1): add Procline300 and 4% polyethylene glycol 6000 at a final concentration of 0.1% to 50mmol / L Tris buffer solution (PH=8.0), mix well and dissolve, pass through a 0.22um filter membrane After filtration, reagent R1 was prepared.

[0039] Preparation method of reagent 2 (R2): procalcitonin antibody was entrusted to a professional company to prepare it according to conventional methods. The antibody has a specific immune reaction with procalcitonin, and has no cross-immune reaction with other antigens, which can meet the needs of this experiment.

[0040] Add EADC:Sulfo-NHS):nano latex particles (300nm or 30nm) in the MES buffer solution with pH=6.0, the mass ratio is 1:0.5:0.1, sonicate for 2-3 seconds, mix well, and shake at room temperature Activate on bed for 2 hours, centrifuge at 17000rpm for 30 minutes, wash 3 times with MES buffer of PH=6.0, then suspend the microspheres in HEPE...

example 2

[0043] Preparation of procalcitonin assay kit

[0044] The preparation method of reagent 1 (R1): in 40mmol / L sodium phosphate buffer (equal concentration of NaH 2 PO 4 and Na 2 HPO 4 Preparation, 0.1% sodium azide, 2% polyethylene glycol 2000, 0.5% EDTA.2Na, 0.5% BSA were added to the final concentration of 0.1% (PH=8.0), mixed and dissolved thoroughly, and filtered through a 0.22um filter membrane to prepare Reagent R1.

[0045]Preparation method of reagent 2 (R2): procalcitonin antibody was entrusted to a professional company to prepare it according to conventional methods. The antibody has a specific immune reaction with procalcitonin, and has no cross-immune reaction with other antigens, which can meet the needs of this experiment.

[0046] Add EADC:Sulfo-NHS):nanolatex particles (400nm or 40nm) to the MES buffer solution with pH=6.0, the mass ratio is 1:0.5:0.1, ultrasonic for 3 seconds, mix well, and activate on a rotary shaker at room temperature Centrifuge at 16,...

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Abstract

The invention particularly relates to a kit for measuring procalcitonin and a method for measuring the content of procalcitonin. The procalcitonin assay kit includes reagent R1 and reagent R2, wherein, reagent R1 is a reaction liquid mixed with various surfactants to promote antibody antigen-specific reaction; reagent R2 is a nano Latex particle buffer, the composition includes 0.1%-2% nano latex particles labeled with anti-human procalcitonin antibody. The detection sensitivity of the kit of the invention can reach 0.12ng / ml, which is higher than the analysis sensitivity of similar products. It is a high-sensitivity, good-specificity, high-accuracy, good-precision nano-latex microparticle procalcitonin assay kit. The latex particles of different particle sizes are cross-linked with polyclonal antibodies to form conjugates of various particle sizes, and the conjugates of different particle sizes are mixed in different proportions, which can broaden the linear range of the procalcitonin assay kit. Improve detection sensitivity.

Description

(1) Technical field [0001] The invention belongs to the field of medical testing, and is an in vitro diagnostic reagent for quantitatively detecting the content of procalcitonin (PCT) in human blood or serum, in particular to a procalcitonin assay kit and procalcitonin method for the determination of content. (2) Background technology [0002] At present, the experimental method for detecting PCT includes radioimmunoassay method, which uses artificially synthesized polyclonal antibodies to specifically recognize and link the synthetic amino acid procalcitonin. This method can detect the mixture of free PCT, bound PCT and calcitonin gene-related peptide precursor, but cannot distinguish the above three substances. The detection time of this method is long (19-22 hours), and there is contamination of radioactive elements. [0003] The double-antibody sandwich immunochemiluminescence method uses double monoclonal antibodies, one of which is a calcitonin antibody and the other...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/6803
Inventor 张立民王琳王元略
Owner SHANDONG SHENGBAILING MEDICAL TECH CO LTD
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