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A kind of preparation method and application of Mortierella chinensis mycelia extract

A technology of Chinese hairy spores and mycelium, applied in the direction of application, medical preparations containing active ingredients, drug combinations, etc., can solve the problems of industrialized resources and habitat destructive damage, resource scarcity, etc., and achieve remarkable efficacy and activity , high safety and stable properties

Active Publication Date: 2020-06-16
HUZHOU PLATEAU BIOLOGICAL RESOURCE IND INNOVATION CENT OF NORTHWEST INST OF PLATEAU BIOLOGY CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitation of the growth cycle and growth environment of wild Cordyceps sinensis itself, it is characterized by scarcity of resources, and industrialization has destructive damage to its resources and habitats

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Preparation and detection of Mortierella chinensis mycelia extract

[0020] (1) Preparation of Mortierella chinensis mycelia extract

[0021] Mortierella sinensis mycelium is dried, pulverized, and passed through a 60-mesh sieve to obtain Mortierella sinensis powder; mix the Mortierella sinensis powder with 50% ethanol solution of the extraction solvent, place it in a microwave extractor, and microwave power 500W, solid-to-liquid ratio 1:20g / mL, extraction time 45min, extraction temperature 60°C, extract 1 time to obtain microwave extract of Mortierella chinensis mycelium; concentrate the extract under reduced pressure, and put it on the D101 large scale Pore ​​adsorption resin, static adsorption for 24h, sequentially eluted with water, 30% (v / v) and 90% (v / v) ethanol, collected 90% (v / v) ethanol eluate, concentrated under reduced pressure, dried to obtain Mortierella sinensis mycelium extract.

[0022] (2) Detection of Mortierella chinensis mycelium extract

[0023]...

Embodiment 2

[0028] Preparation and detection of Mortierella chinensis mycelia extract

[0029] (1) Preparation of Mortierella chinensis mycelia extract

[0030] Mortierella sinensis mycelium is dried, pulverized, and passed through a 60-mesh sieve to obtain Mortierella sinensis powder; mix the Mortierella sinensis powder with 70% (v / v) ethanol solution of the extraction solvent, and place in microwave extraction In the instrument, the microwave power is 600W, the solid-liquid ratio is 1:40g / mL, the extraction time is 15min, and the extraction temperature is 40°C, and the extraction is performed 3 times to obtain the microwave extract of Mortierella sinensis mycelia; the extract is concentrated under reduced pressure , on D101 type macroporous adsorption resin, statically adsorbed for 12h, eluted with water, 50% (v / v) and 80% (v / v) ethanol in turn, collected 80% (v / v) ethanol eluate, and depressurized Concentrate and dry to obtain Mortierella sinensis mycelium extract.

[0031] (2) Detec...

Embodiment 3

[0037] Preparation and detection of Mortierella chinensis mycelia extract

[0038] (1) Preparation of Mortierella chinensis mycelia extract

[0039] Mortierella sinensis mycelium is dried, pulverized, and passed through a 60-mesh sieve to obtain Mortierella sinensis powder; mix the Mortierella sinensis powder with 60% (v / v) ethanol solution of the extraction solvent, and place in microwave extraction In the instrument, the microwave power is 550W, the solid-liquid ratio is 1:30g / mL, the extraction time is 30min, and the extraction temperature is 50°C, and extraction is performed twice to obtain the microwave extract of Mortierella sinensis mycelia; the extract is concentrated under reduced pressure , on D101 type macroporous adsorption resin, static adsorption for 18h, followed by water, 40% (v / v) and 85% (v / v) ethanol for elution, collecting 85% (v / v) ethanol eluate, decompression Concentrate and dry to obtain Mortierella sinensis mycelium extract.

[0040] (2) Detection of...

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PUM

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Abstract

The invention relates to a hirsutella sinensis mycelium extract preparation method and application of a hirsutella sinensis mycelium extract in an aspect of resisting oxidation. The extract is obtained by smashing hirsutella sinensis mycelium, extracting the hirsutella sinensis mycelium, adsorbing the hirsutella sinensis mycelium through macroporous resin, concentrating the hirsutella sinensis mycelium and drying the hirsutella sinensis mycelium. The extract is a natural, microorganism-source and nontoxic antioxidant. Detection shows that the scavenging free radical IC50 of the extract is 0.052+ / -0.003mg / mL, and the activity of the extract reaches 1 / 10 of Vc; the extract can serve as an active ingredient to be manufactured into various medical forms of antioxidant products, has the characteristics of being remarkable in effective activity and clear in function mechanism and is stable in property, certain in ingredient, high in safety and high in reliability. The hirsutella sinensis mycelium extract can be applied to antioxidant medicines, health-care food and food.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine. It specifically relates to the preparation method of Mortierella mycelia extract and its application in anti-oxidation. Background technique [0002] Trichoderma sinensis ( Hirsutella sinensis ) is an asexual strain of Cordyceps isolated from natural Cordyceps. Due to the limitation of the growth cycle and growth environment of wild Cordyceps sinensis itself, it is characterized by scarcity of resources, and industrialization has destructive damage to its resources and habitat. Therefore, it is of great significance to the development and industrialization of the medicinal value of Cordyceps sinensis to obtain the mycelia of Cordyceps sinensis by artificially separating and cultivating the strains. [0003] Modern pharmacological research results show that Cordyceps sinensis has obvious antioxidant effects. Experiments have confirmed that both natural Cordyceps sinensis a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/068A61P39/06A23L33/105
CPCA23V2002/00A61K36/068A61K2236/333A61K2236/51A61K2236/55A23V2200/302A23V2200/308A23V2250/208
Inventor 王洪伦韩发刘鑫年邓黎
Owner HUZHOU PLATEAU BIOLOGICAL RESOURCE IND INNOVATION CENT OF NORTHWEST INST OF PLATEAU BIOLOGY CHINESE ACADEMY OF SCI
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