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Purification technology of sweetberry honeysuckle anthocyanin

A kind of fruit anthocyanin, indigo fruit technology, applied in the purification process of indigo fruit anthocyanin, international patent A23L1/29 "in the field of nutritional products, can solve problems such as toxicity and harm to health, and achieve high recovery rate and high purity. Effect

Inactive Publication Date: 2016-08-24
NORTHEAST FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, most of the pigments used in the food industry are synthetic pigments, almost all of which are toxic to varying degrees. Long-term use will endanger people's health. Therefore, natural pigments have attracted more and more attention in the field of scientific research.
However, since there is no pure anthocyanin in the domestic market so far, the extraction of high-purity anthocyanin provides the necessary characterization conditions and theoretical basis for the in-depth research and development of anthocyanin pigments, and is conducive to its industrial utilization.

Method used

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  • Purification technology of sweetberry honeysuckle anthocyanin

Examples

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Effect test

Embodiment 1

[0020] The purification process of a high-purity Lonicera edulis anthocyanin described in this embodiment includes the following steps:

[0021] 1. Preparation of Lonicera edulis anthocyanin concentrate

[0022] Take 1kg of pretreated clean and high-quality Lonicera edulis and soak it in 2L (solid-to-liquid ratio 1:2) of 70% ethanol, first crush it with a beating machine for 5 minutes, then homogenize it with a high-shear dispersing emulsifier for 3 minutes, and centrifuge The machine performs solid-liquid separation. The centrifuge performs centrifugal separation under the conditions of 3000r / min and 10min to obtain the supernatant, which is vacuum concentrated. The vacuum concentration conditions are controlled at -0.01KPa, 50℃, and concentrated to one-fifth of the original volume ( 0.4L).

[0023] 2. Purification of Lonicera edulis Anthocyanins

[0024] Add 0.1% edible citric acid-added Lonicera edulis concentrated juice to the pre-treated macroporous adsorption resin AB-8 chromat...

Embodiment 2

[0028] The purification process of a high-purity Lonicera edulis anthocyanin described in this embodiment includes the following steps:

[0029] 1. Preparation of Lonicera edulis anthocyanin concentrate

[0030] Take 1kg of pretreated clean and high-quality Lonicera edulis and soak it in 3L (solid-to-liquid ratio 1:3) of 80% ethanol, crush it with a beating machine for 6 minutes, then homogenize it with a high-shear dispersing emulsifier for 5 minutes, and centrifuge The machine performs solid-liquid separation. The centrifuge performs centrifugal separation under the conditions of 3000r / min and 10min to obtain the supernatant, which is vacuum concentrated. The vacuum concentration conditions are controlled at -0.01KPa, 50℃, and concentrated to one-sixth of the original volume ( 0.5L).

[0031] 2. Purification of Lonicera edulis Anthocyanins

[0032] Add 0.15% edible citric acid Lonicera edulis concentrated juice to the pre-treated macroporous adsorption resin AB-8 chromatography col...

Embodiment 3

[0036] The purification process of a high-purity Lonicera edulis anthocyanin described in this embodiment includes the following steps:

[0037] 1. Preparation of Lonicera edulis anthocyanin concentrate

[0038] Take 1kg of pretreated clean, high-quality Lonicera edulis and soak it in 1.5L (solid-to-liquid ratio 2:3) of 60% ethanol, crush it with a beating machine for 10 minutes, and homogenize it with a high-shear dispersing emulsifier for 7 minutes. The centrifuge performs solid-liquid separation. The centrifuge performs centrifugal separation under the conditions of 3000r / min and 10min to obtain the supernatant, which is vacuum concentrated. The vacuum concentration conditions are controlled at -0.01KPa, 50℃, and concentrated to one-fifth of the original volume. (0.3L).

[0039] 2. Purification of Lonicera edulis Anthocyanins

[0040] Add 0.5% edible citric acid to Lonicera edulis concentrated juice into the pre-treated macroporous adsorption resin AB-8 chromatography column, the ...

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Abstract

The invention provides a purification technology of sweetberry honeysuckle anthocyanin, and belongs to the technical field of international patent A23L1 / 29 nutrient products. The purification technology mainly aims at improving the purification purity of the sweetberry honeysuckle anthocyanin and solving the comprehensive utilization problem of sweetberry honeysuckles. The technology is characterized by mainly comprising the steps that the sweetberry honeysuckles are extracted with ethanol of 60% to 80%, crushing is conducted through a beating machine and a high-shear emulsifying machine, 0.1%-1.5% of edible citric acid is added in the extraction process, and the structure of the anthocyanin can be effectively protected; a macroporous adsorption resin adsorption technology is adopted, ethanol of 0% to 100% is adopted for gradient elution sequentially, eluents are collected separately and vacuum-frozen into crystalline powder, and the purity of the obtained sweetberry honeysuckle anthocyanin reaches up to 35% or above. The technology is simple and easy to operate, the purity of the obtained product is high, the repeatability is good, the production cost is low, and the production requirements of multiple enterprises can be met.

Description

Technical field [0001] The invention relates to a process for purifying anthocyanins, in particular to a process for purifying anthocyanins from Lonicera edulis, belonging to the technical field of international patent A23L1 / 29 "nutritional products". Background technique [0002] Lonicera edulis, also known as Lonicera edulis, is a dicotyledonous plant of the family Honeysuckle. The fruit is a berry, dark blue, white powder, oval or oblong, and the juice is a bright deep rose color and rich in anthocyanins. Anthocyanins, also known as anthocyanins, are water-soluble natural pigments widely found in plants in nature and belong to flavonoids. Anthocyanins bring many benefits to the human body. Fundamentally speaking, anthocyanins are a powerful antioxidant that can protect the human body from harmful substances called free radicals. Anthocyanins can also enhance the elasticity of blood vessels, improve the circulatory system and increase the smoothness of the skin, inhibit inflam...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D311/62C09B61/00A23L1/29
Inventor 樊梓鸾张艳东柳雅馨
Owner NORTHEAST FORESTRY UNIVERSITY
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