Preparation method of regenerated silk fibroin membrane

A technology for regenerating silk and fibroin, which is applied in the polymer field, can solve the problems of many organic solvents, many operation steps, destruction of biological characteristics, etc., and achieves the effects of low production cost, simple and convenient operation process, and short production cycle.

Active Publication Date: 2016-08-24
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims at the existing silk fibroin film preparation method which requires secondary treatment to produce a silk film that is insoluble in water or has other characterist

Method used

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  • Preparation method of regenerated silk fibroin membrane
  • Preparation method of regenerated silk fibroin membrane
  • Preparation method of regenerated silk fibroin membrane

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Weigh 5.00 g of shredded Thai silk cocoon shells, put them into 2000 ml of 0.21 wt % sodium bicarbonate boiling aqueous solution for degumming treatment for 30 min. After repeated washing with deionized water for 2-3 times, move to a ventilated place to dry naturally to obtain degummed Thai silk.

[0029] Calcium chloride-formic acid solution with a concentration of 1.5wt% was prepared and 1.90g of the solution was weighed, placed in a 2ml centrifuge tube, and then 0.17g of degummed Thai silk was placed in the solution for dissolution, and the Thai silk was found within 2 minutes. Can be dissolved completely, just can get Thai silk protein solution.

[0030] Filtrate the Thai silk protein solution with a PTFE microporous filter membrane with a pore size of 0.45 μm, cast the filtrate on PDMS, and then place it in a 45°C oven to dry for 5 hours to prepare the Thai silk protein membrane.

Embodiment 2

[0032] Weigh 5.00 g of shredded Thai silk cocoon shells, put them into 2000 ml of 0.21 wt % sodium bicarbonate boiling aqueous solution for degumming for 30 min. After repeated washing with deionized water for 2-3 times, move to a ventilated place to dry naturally to obtain degummed Thai silk.

[0033]Prepare a calcium chloride-formic acid solution with a concentration of 2.0wt% and weigh 1.90g, put it in a 2ml centrifuge tube, and then put 0.17g of degummed Thai silk in the solution to dissolve it. It takes 1 minute to find the Thai silk. After being dissolved completely, Thai silk protein solution can be obtained.

[0034] Filtrate the Thai silk protein solution with a PTFE microporous filter membrane with a pore size of 0.45 μm, cast the filtrate on PDMS, and then place it in a 45°C oven to dry for 5 hours to prepare the Thai silk protein membrane.

Embodiment 3

[0036] Weigh 5.00 g of shredded Thai silk cocoon shells, put them into a boiling aqueous solution of sodium bicarbonate with a concentration of 0.21 wt%, and perform degumming for 30 min. After repeated washing with deionized water for 2-3 times, move to a ventilated place to dry naturally to obtain degummed Thai silk.

[0037] Prepare a calcium chloride-formic acid solution with a concentration of 6.0wt%, weigh 1.90g of the solution, place it in a 2ml centrifuge tube, and then dissolve 0.17g of degummed Thai silk in the solution, and find the Thai silk within 30s Just can be dissolved completely, just can obtain Thai silk protein solution.

[0038] Filtrate the Thai silk protein solution with a PTFE microporous filter membrane with a pore size of 0.45 μm, cast the filtrate on PDMS, and then place it in a 45°C oven to dry for 5 hours to prepare the Thai silk protein membrane.

[0039] (1) Comparison of surface morphology and structure of silk protein membranes

[0040] figu...

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Abstract

The invention discloses a preparation method of a regenerated silk fibroin membrane. The method comprises the following steps: shearing Thai silk cocoons, then putting the sheared Thai silk cocoons into a sodium hydrogen carbonate solution for carrying out degumming treatment, washing and drying the Thai silk cocoons subjected to degumming treatment; putting the degummed and dried Thai silk into a calcium chloride-formic acid solution, and quickly dissolving the Thai silk to form a silk fibroin solution; and drying the filtered silk fibroin solution to obtain the regenerated silk fibroin membrane. Through the method, the complicated process of preparing the membrane in a conventional method can be avoided; the treatment difficulty of organic wastewater formed after production can be reduced; the method is developed towards an environment-friendly direction; the smooth, water-insoluble and high-heat-stability silk membrane can be prepared by adjusting the concentration of calcium chloride. The regenerated silk fibroin membrane is high in biocompatibility and different in transparency, smooth flatness, water solubility and heat stability; through flexible regulation of the regenerated silk fibroin membrane according to the performance needed for the product, the regenerated silk fibroin membrane can be better applied to chemical, pharmaceutical and food industries.

Description

technical field [0001] The invention belongs to the field of macromolecules, and relates to a processing method of a regenerated silk protein film, especially in the production method of the silk protein film, which shows the characteristics of simplicity and high efficiency, and at the same time, the method can regulate the structure and thermal stability of the film. The invention can provide certain experimental and theoretical basis for the research and application of the silk protein in the fields of chemical industry, medicine, food and the like. Background technique [0002] Silk fibroin, also known as silk fibroin, is a fibrous protein extracted from silkworm cocoons by degumming. It is composed of 18 amino acids, mainly glycine, alanine, serine and tyrosine, which are closely related to the keratin protein of human skin. The amino acids in are very close. In addition, these 18 kinds of amino acids contain 8 kinds of amino acids necessary for the human body. Silk p...

Claims

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Application Information

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IPC IPC(8): C08J5/18C08L89/00
CPCC08J5/18C08J2389/00
Inventor 王昉于海洋顾正桂
Owner NANJING NORMAL UNIVERSITY
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