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Nattokinase composition for dissolving thrombus and preparation method thereof

A technology of nattokinase and composition, applied in the field of nattokinase composition and preparation thereof, can solve the problems such as the hemorrhagic side effect of re-embolization caused by anti-reperfusion, heavy economic burden and high price of thrombosis patients, etc., and can prolong arterial thrombosis. The effect of formation time, reduction of venous thrombosis weight, and economic pressure reduction

Inactive Publication Date: 2016-08-31
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Thrombolytic drugs such as streptokinase, urokinase, and tissue plasminogen activator commonly used in the domestic market have obvious deficiencies: anti-reperfusion, re-embolization and hemorrhagic side effects, and are expensive
For the application of nattokinase products, nutrition experts recommend a daily preventive dose of 1500-2000 IU, but this dosage will also bring higher costs, making the economic burden of thrombosis patients heavier

Method used

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  • Nattokinase composition for dissolving thrombus and preparation method thereof
  • Nattokinase composition for dissolving thrombus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] The composition is made up of nattokinase, angelica and epimedium, wherein, the consumption of nattokinase is calculated by nattokinase fibrinolytic activity, and the fibrinolytic activity of nattokinase in every g composition is 200IU; The dosage is calculated by icariin, and the content of icariin in the composition is 0.03 mg / g; the dosage of angelica is calculated by ferulic acid, and the content of ferulic acid in the composition is 0.02 mg / g.

[0041] The preparation method of this composition is:

[0042] 1) centrifuging the nattokinase fermentation liquid at 3000rpm, concentrating the supernatant by ultrafiltration at room temperature, the molecular weight cut-off of the ultrafiltration membrane is 50000, and the ultrafiltration pressure is 0.1MPa, to obtain a nattokinase concentrated solution;

[0043] 2) extracting Epimedium with water, filtering the extract, and concentrating the filtrate into Epimedium extract with a relative density of 1.10 at 60°C;

[004...

Embodiment 2

[0047] The composition is made up of nattokinase, Angelica sinensis and Epimedium, wherein the consumption of Nattokinase is based on the fibrinolytic activity of nattokinase, and the fibrinolytic activity of nattokinase in every g composition is 400IU; The dosage is calculated by icariin, and the content of icariin in the composition is 0.04 mg / g; the dosage of angelica is calculated by ferulic acid, and the content of ferulic acid in the composition is 0.03 mg / g.

[0048] The preparation method of this composition is:

[0049] 1) centrifuging the nattokinase fermented liquid at 6000rpm, concentrating the supernatant by ultrafiltration at normal temperature, the molecular weight cut-off of the ultrafiltration membrane is 200000, and the ultrafiltration pressure is 0.3MPa to obtain the nattokinase concentrated solution;

[0050] 2) extracting Epimedium with water, and concentrating the extract into Epimedium extract with a relative density of 1.20 at 60°C;

[0051] 3) adding ...

Embodiment 3

[0054] The composition is made up of nattokinase, angelica and epimedium, wherein, the consumption of nattokinase is calculated by nattokinase fibrinolytic activity, and the fibrinolytic activity of nattokinase in every g composition is 300IU; The dosage is calculated by icariin, and the content of icariin in the composition is 0.035 mg / g; the dosage of angelica is calculated by ferulic acid, and the content of ferulic acid in the composition is 0.025 mg / g.

[0055] The preparation method of this composition is:

[0056] 1) centrifuging the nattokinase fermented liquid at 5000rpm, concentrating the supernatant by ultrafiltration at normal temperature, the molecular weight cut-off of the ultrafiltration membrane is 100000, and the ultrafiltration pressure is 0.2MPa to obtain the nattokinase concentrated solution;

[0057] 2) extracting Epimedium with water, and concentrating the extract into Epimedium extract with a relative density of 1.15 at 60°C;

[0058] 3) adding water to...

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Abstract

The invention discloses a nattokinase composition for dissolving thrombus and a preparation method thereof. The composition comprises nattokinase, angelica sinensis and herba epimedii, wherein the fibrinolytic activity of nattokinase in each gram of the composition is 200-400IU. The preparation method comprises the following steps: (1) preparing a nattokinase fermentation broth by utilizing bacillus subtilis capable of producing nattokinase, centrifuging, and concentrating supernate to obtain nattokinase concentrate; (2) extracting herba epimedii with water, and concentrating the extract to obtain herba epimedii extract; (3) extracting angelica sinensis with water, and concentrating the extract to obtain angelica sinensis extract; and (4) mixing the herba epimedii extract, the angelica sinensis extract and the nattokinase concentrate. The nattokinase composition has relatively good effects of thrombosis prevention and thrombolysis since nattokinase, angelica sinensis and herba epimedii serving as raw materials are synergistic.

Description

technical field [0001] The invention relates to a pharmaceutical composition, in particular to a nattokinase composition for dissolving thrombus and a preparation method thereof. Background technique [0002] As a high incidence of middle-aged and elderly people, thrombotic disease is one of the diseases that threaten death. At present, the number of elderly people over 60 years old has reached more than 100 million, accounting for 8.7% of the population. In the next 20 years, my country will enter an aging society. Thrombosis The prevention and treatment of diseases must be given sufficient attention. There are about 15 million patients with thrombosis in the world, and the potential market value of thrombolytic drugs is about 2 billion US dollars. Thrombolytic drugs such as streptokinase, urokinase and tissue plasminogen activator commonly used in the domestic market have obvious deficiencies: anti-reperfusion, re-embolization and hemorrhagic side effects, and are expensiv...

Claims

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Application Information

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IPC IPC(8): A61K38/48A61P7/02A61K36/296
CPCA61K38/482A61K36/232A61K36/296C12Y304/21062A61K2300/00
Inventor 方捷方鹏程康萍
Owner 方捷
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