A metacycline hydrochloride pharmaceutical composition and applications thereof in biomedicines

A technology of metacycline hydrochloride and a composition, applied in the field of biomedicine, can solve the problems such as the related reports on vascular dementia of metacycline hydrochloride and the like, and achieve the effect of improving the therapeutic effect

Inactive Publication Date: 2016-08-31
ZHENJIANG GAOHAI BIOLOGICAL PHARMA CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] So far, there has been no report on the correlation between metacyc

Method used

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  • A metacycline hydrochloride pharmaceutical composition and applications thereof in biomedicines
  • A metacycline hydrochloride pharmaceutical composition and applications thereof in biomedicines
  • A metacycline hydrochloride pharmaceutical composition and applications thereof in biomedicines

Examples

Experimental program
Comparison scheme
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Example Embodiment

[0020] Example 1: Compound (Ⅰ) isolation preparation and structure confirmation

[0021] Separation method: (a) Crush the dry bulbs (2kg) of Fritillaria vulgaris and extract them with 90% ethanol under reflux (15L×3 times). Combine the extracts and concentrate them to no alcohol (3L), and then use petroleum ether ( 3L×3 times), ethyl acetate (3L×3 times), and water-saturated n-butanol (3L×3 times) extraction to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) ) In step (a), the ethyl acetate extract is removed with D101 type macroporous resin, first eluted with 35% ethanol for 8 column volumes, then eluted with 90% ethanol for 12 column volumes, and collect 90% eluate Concentrate under reduced pressure to obtain a 90% ethanol elution concentrate; (c) In step (b), the 90% ethanol elution concentrate is separated with normal phase silica gel, and the volume ratio is 120:1 (11 column volumes), 60 :1 (9 column volumes), 30:1 (9 column vol...

Example Embodiment

[0024] Example 2: Pharmacological effects

[0025] 1. Materials and methods

[0026] 1.1 Animals

[0027] SD rats, half male and half male, weigh (250±20) g (Experimental Animal Center of Anhui Medical University).

[0028] 1.2 Drugs and reagents

[0029] Metacycline hydrochloride was purchased from the National Institute for the Control of Pharmaceutical and Biological Products. Compound (I) is self-made, and the preparation method is shown in Example 1. Ergot dihydrogen mesylate sustained-release capsules (trade name: Peleneng, 2.5 mg, Sanofi-Aventis); Adenosine Diphosphate (ADP, Sigma); Thrombin (Sigma); Epinephrine Injection (Shanghai Hefeng Pharmaceutical Co., Ltd.); Kangpaite Medical Glue (Beijing Shunkang Medical Glue Co., Ltd.); Penicillin Sodium for Injection (Zhongnuo Pharmaceutical Shijiazhuang Co., Ltd.); Gentamicin Injection (Nanjing Jinling Pharmaceutical Co., Ltd.) Factory); Acetylcholine (Ach) determination kit (R&D company).

[0030] 1.3 Instrument

[0031] RM-200 eig...

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Abstract

A metacycline hydrochloride pharmaceutical composition and applications thereof in biomedicines are disclosed. The composition comprises metacycline hydrochloride and a natural product compound (I) having a novel structure and separated from dry bulbs of fritillaria thunbergii. When the metacycline hydrochloride and the compound (I) are used separately, learning and memory capabilities of rats with synthetic vascular dementia can be significantly improved. When the metacycline hydrochloride and the compound (I) are used together, the improving effects are more obvious. The composition can be developed into a medicine treating the synthetic vascular dementia.

Description

technical field [0001] The invention belongs to the field of biomedicine and relates to a new application of metacycline hydrochloride, in particular to a pharmaceutical composition of metacycline hydrochloride and its application in biomedicine. Background technique [0002] Metacycline hydrochloride, also known as methacycline hydrochloride, methacycline, methacycline, is a semi-synthetic oxytetracycline, which is effective against G+ and G- bacteria, rickettsia, actinomycetes, and Chlamydia trachomatis , protozoa, etc. have inhibitory effect. t1 / 2=14.3h. The antibacterial performance is stronger than tetracycline, and the absorption is good. The application is the same as tetracycline. [0003] Vascular dementia (VD) is a persistent impairment of acquired intelligence that occurs on the basis of cerebrovascular diseases, mainly with memory and cognitive impairments, or with language, visual-spatial skills, and emotional or personality disorders. [0004] So far, there h...

Claims

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Application Information

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IPC IPC(8): C07J63/00A61K31/56A61K31/65A61P25/28
CPCC07J63/008A61K31/56A61K31/65A61K36/8967A61K2300/00
Inventor 潘立李义高李小彪赵阳
Owner ZHENJIANG GAOHAI BIOLOGICAL PHARMA CO LTD
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