A metacycline hydrochloride pharmaceutical composition and applications thereof in biomedicines
A technology of metacycline hydrochloride and a composition, applied in the field of biomedicine, can solve the problems such as the related reports on vascular dementia of metacycline hydrochloride and the like, and achieve the effect of improving the therapeutic effect
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[0020] Example 1: Compound (Ⅰ) isolation preparation and structure confirmation
[0021] Separation method: (a) Crush the dry bulbs (2kg) of Fritillaria vulgaris and extract them with 90% ethanol under reflux (15L×3 times). Combine the extracts and concentrate them to no alcohol (3L), and then use petroleum ether ( 3L×3 times), ethyl acetate (3L×3 times), and water-saturated n-butanol (3L×3 times) extraction to obtain petroleum ether extract, ethyl acetate extract and n-butanol extract respectively; (b) ) In step (a), the ethyl acetate extract is removed with D101 type macroporous resin, first eluted with 35% ethanol for 8 column volumes, then eluted with 90% ethanol for 12 column volumes, and collect 90% eluate Concentrate under reduced pressure to obtain a 90% ethanol elution concentrate; (c) In step (b), the 90% ethanol elution concentrate is separated with normal phase silica gel, and the volume ratio is 120:1 (11 column volumes), 60 :1 (9 column volumes), 30:1 (9 column vol...
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[0024] Example 2: Pharmacological effects
[0025] 1. Materials and methods
[0026] 1.1 Animals
[0027] SD rats, half male and half male, weigh (250±20) g (Experimental Animal Center of Anhui Medical University).
[0028] 1.2 Drugs and reagents
[0029] Metacycline hydrochloride was purchased from the National Institute for the Control of Pharmaceutical and Biological Products. Compound (I) is self-made, and the preparation method is shown in Example 1. Ergot dihydrogen mesylate sustained-release capsules (trade name: Peleneng, 2.5 mg, Sanofi-Aventis); Adenosine Diphosphate (ADP, Sigma); Thrombin (Sigma); Epinephrine Injection (Shanghai Hefeng Pharmaceutical Co., Ltd.); Kangpaite Medical Glue (Beijing Shunkang Medical Glue Co., Ltd.); Penicillin Sodium for Injection (Zhongnuo Pharmaceutical Shijiazhuang Co., Ltd.); Gentamicin Injection (Nanjing Jinling Pharmaceutical Co., Ltd.) Factory); Acetylcholine (Ach) determination kit (R&D company).
[0030] 1.3 Instrument
[0031] RM-200 eig...
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