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Method for extracting and separating polysaccharides from Celosia cristata

A technology for cockscomb and polysaccharide is applied in the field of extracting and separating polysaccharides from cockscomb, can solve the problems of destruction of effective components, low polysaccharide purity, large amount of solvent, etc., achieves high yield and content of functional components, simple preparation process, The effect of less solvent consumption

Active Publication Date: 2016-08-31
SCI GREEN SHANDONG ENVIRONMENT TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, according to literature search, extraction and purification of plant polysaccharides, and the method of obtaining polysaccharides containing polysaccharides are achieved by high-temperature extraction, sevag deproteinization, and resin column chromatography enrichment and purification. These extraction methods often have a large amount of solvent and a long extraction time. , low work efficiency and other disadvantages, especially during the preparation process, it is necessary to repeatedly soak the extract, extract or eluent. Due to more heating and concentration, it is easy to cause damage or loss of active ingredients, resulting in the extraction of the obtained extract. The efficiency and the transfer rate of active ingredients are not high, and harmful solvents such as n-butanol with a high boiling point are used, causing pollution to the environment
In addition, the traditional ethanol precipitation is easy to cause the precipitation of impurities such as protein, so that the purity of polysaccharide is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: the method for separating polysaccharide in Celosia

[0019] (1) Pretreatment: Take 1.0Kg of fresh cockscomb, wash it with water, smash it, squeeze it, and filter it to obtain 245g of polysaccharide solution;

[0020] (2) Egg white treatment: Take the solution in step (1), under stirring conditions, take a small hole in the shell of fresh eggs, slowly add egg white until the final concentration of egg white is 65mg / L, stir for 30min, and let it stand for 12h; filter , the supernatant is 215g of crude polysaccharide;

[0021] (3) Decolorization: heat up the crude polysaccharide solution obtained in step (2) to 50°C, add 1.3 g of a mixture of diatomaceous earth and activated carbon, the ratio of which is 1:1, process for 30 minutes under stirring conditions, and filter;

[0022] (4) Precipitation: Concentrate the solution obtained in step (3) to 1 / 5 of the original volume, slowly add 95% ethanol dropwise under stirring conditions, until white precipitate ap...

Embodiment 2

[0023] Embodiment 2: the method for separating polysaccharide in Celosia

[0024] (1) Pretreatment: Take 1.1Kg of fresh cockscomb, wash it with water, smash it, squeeze it, and filter it to obtain 258g of polysaccharide solution;

[0025] (2) Egg white treatment: take the solution in step (1), under stirring conditions, take a small hole in the shell of fresh eggs, slowly add egg white until the final concentration of egg white is 75mg / L, stir for 30min, and let it stand for 14h; filter , 238g of crude polysaccharide solution;

[0026] (3) Decolorization: heat up the crude polysaccharide solution obtained in step (2) to 55°C, add 1.5g of a mixture of diatomaceous earth and activated carbon, the ratio of which is 3:2, process for 40min under stirring conditions, and filter;

[0027] (4) Precipitation: Concentrate the solution obtained in step (3) to 1 / 5 of the original volume, slowly add 95% ethanol dropwise under stirring conditions, until a white precipitate appears, stop st...

Embodiment 3

[0028] Embodiment 3: the method for separating polysaccharide in Celosia

[0029] (1) Pretreatment: Take 1.5Kg of fresh cockscomb, wash it with water, smash it, squeeze it, and filter it to obtain 261g of polysaccharide solution;

[0030] (2) Egg white treatment: Take the solution in step (1), under stirring conditions, take a small hole in the shell of fresh eggs, slowly add egg white until the final concentration of egg white is 80mg / L, stir for 30min, and let it stand for 15h; filter , the supernatant is 240g of crude polysaccharide;

[0031] (3) Decolorization: heat up the crude polysaccharide solution obtained in step (2) to 60°C, add 1.5g of a mixture of diatomaceous earth and activated carbon, the ratio of which is 2:1, process for 60min under stirring conditions, and filter;

[0032] (4) Precipitation: Concentrate the solution obtained in step (3) to 1 / 5 of the original volume, slowly add 95% ethanol dropwise under stirring conditions, until a white precipitate appear...

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Abstract

The invention relates to the technical field of polysaccharide separation and purification, and particularly discloses a method for extracting and separating polysaccharides from Celosia cristata. By using Celosia cristata as the raw material, the method is characterized by comprising the following steps: cleaning the Celosia cristata, mashing, pressing, and filtering to obtain a polysaccharide solution; adding egg white into the polysaccharide solution while stirring, standing, and filtering, wherein the supernatant is a crude polysaccharide solution; heating the crude polysaccharide solution, adding an adsorbent for decolorization, and filtering; and concentrating the filtered solution, dropwisely adding an ethanol solution while stirring until a white precipitate appears, stopping stirring, standing, filtering, and drying the precipitate to obtain the polysaccharides. The method is simple in technique and convenient to operate, has the advantages of low equipment investment, low solvent consumption, no environmental pollution, low production cost, high yield and high effective component content, and provides a new concept for developing the medicinal value of Celosia cristata and implementing industrialization of Celosia cristata polysaccharides.

Description

(1) Technical field [0001] The invention relates to the technical field of separation and purification of polysaccharides, in particular to a method for extracting and separating polysaccharides from Celosia celosia. (2) Background technology [0002] Plant polysaccharides, also known as plant polysaccharides, are polysaccharides with a degree of polymerization of more than 10 produced by plant cell metabolism. They are commonly found in plants in nature, including starch, fiber, polysaccharides, and pectin. Due to the wide range of sources of plant polysaccharides, the molecular composition and molecular weight of different plant polysaccharides are different. Some plant polysaccharides, such as starch, cellulose, and pectin, have already become an important part of people's daily life. [0003] Nowadays, the research on plant polysaccharides is attracting more and more attention, and the international scientific community even proposes that the 21st century is the century...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 田沛霖史海岭赵安琪
Owner SCI GREEN SHANDONG ENVIRONMENT TECH CO LTD
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