Assembly type multi-condition parallel-culture microfluidic control device and using method thereof

Abstract

The invention provides an assembly type multi-condition parallel-culture microfluidic control device which is composed of a microfluidic control chip and a cover plate, wherein the two parts can be flexibly combined, and are conveniently dismounted; the microfluidic control chip comprises a cell culture room array, a liquor inlet tank, a waste liquor tank and a microfluidic channel; the cell culture room array, the liquor inlet tank and the waste liquor tank communicate with one another through the microfluidic channel; and the cover plate detachably covers the cell culture room array. The microfluidic control device is strong in universality, is convenient to operate, and has a wide application prospect in the fields of cell treatment, tumor drug evaluation, and the like.

Description

technical field [0001] The invention belongs to the field of tissue engineering-cell microenvironment research, and in particular relates to an assembly-type multi-condition parallel culture microfluidic device. Background technique [0002] Cells are very sensitive to the stimuli of various physical and chemical factors in the microenvironment in the body, and can undergo cell fate changes under the influence of the latter. Therefore, the study of cellular microenvironment has been paid more and more attention. For most cells in vivo, microenvironmental factors include: cellular heterogeneity, cell compartmentation, three-dimensional configuration, extracellular matrix proteins, soluble factors, blood vessels, and other types of cells that make up the microenvironment. At present, in vitro models used to construct bionic microenvironments are helpful to understand the influence of in vivo microenvironmental factors on cells. Huge advantage. Existing methods include: usin...

AI Technical Summary

Problems solved by technology

However, the existing technology has the following defects: the introduction of exogenous biological materials or biological factors may lead to immune rejection, low biocompatibility and low biodegradability; Precise spatiotemporal control of the environment and effective biological evaluation, in vitro biomimetic construction of cell microenvironment and research on its impact on cells are still limited

However, most of the existing work on cell microenvironment based on microfluidics is focused on single-factor research, or the construction of two-dimensional cell models in microchannels inside the chip, which has not yet fully reflected the essential advantages of the chip, and lacks the specific microenvironment for cells. Comprehensive study of fate determination and high-throughput screening of optimal culture conditions

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