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Biological enzymolysis production method for controllable narrow molecular weight functional chitosan oligosaccharide

A technology with narrow molecular weight and production method, applied in the field of biological enzymatic hydrolysis production, can solve the problems of low effective content of chitosan oligosaccharide, unstable molecular weight, long enzymatic hydrolysis time, etc. high effect

Inactive Publication Date: 2016-08-31
潍坊华诺生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process of preparing chitosan oligosaccharides by enzymatic degradation is obviously superior to the other two chemical degradation methods, but it generally has problems such as low effective content of chitosan oligosaccharides, low degree of deacetylation, unstable molecular weight, long enzymatic hydrolysis time, and high energy consumption.

Method used

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  • Biological enzymolysis production method for controllable narrow molecular weight functional chitosan oligosaccharide
  • Biological enzymolysis production method for controllable narrow molecular weight functional chitosan oligosaccharide
  • Biological enzymolysis production method for controllable narrow molecular weight functional chitosan oligosaccharide

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Embodiment 1

[0036] Example 1 A biological enzymolysis production method of controllable narrow molecular weight functional chitosan oligosaccharide

[0037] Include the following steps:

[0038] Step 1: Media Preparation

[0039] (1) Chitosan medium preparation:

[0040] Chitosan medium, in parts by weight, comprises the following raw materials:

[0041] 15 parts of chitosan, 300 parts of 1.3% acetic acid solution, (NH 4 ) 2 SO 4 3 parts of powder, 20 parts of skimmed milk powder, 500 parts of sterile water;

[0042] Dissolve chitosan in acetic acid solution, then add (NH 4 ) 2 SO 4 powder, skimmed milk powder and sterile water to prepare chitosan medium.

[0043] (2) Preparation of strain fermentation medium:

[0044] Strain fermentation medium, calculated in parts by weight, includes the following raw materials:

[0045] 0.5 parts of soybean flour, 0.03 parts of red algae culture solution, 1.2 parts of rice straw extract, 0.15 parts of corn flour, 0.6 parts of egg white liqui...

Embodiment 2

[0072] Example 2 A biological enzymolysis production method of controllable narrow molecular weight functional chitosan oligosaccharide

[0073] Step 1: Media Preparation

[0074] (1) Chitosan medium preparation:

[0075] Described chitosan culture medium, by weight, raw material comprises:

[0076] 16 parts of chitosan, 350 parts of 1.4% acetic acid solution, (NH 4 ) 2 SO 4 4 parts of powder, 21 parts of skimmed milk powder, 600 parts of sterile water;

[0077] Dissolve chitosan in acetic acid solution, then add (NH 4 ) 2 SO 4 Powder, skimmed milk powder and sterile water are sterilized to prepare chitosan medium.

[0078] (2) Preparation of strain fermentation medium:

[0079] The strain fermentation medium, in parts by weight, raw materials include:

[0080] 0.6 parts of soybean flour, 0.035 parts of red algae culture solution, 1.4 parts of rice straw infusion, 0.17 parts of corn flour, 0.7 parts of egg white liquid, 0.09 parts of pork bone broth, 0.3 parts of cal...

Embodiment 3

[0102] Example 3 A biological enzymatic production method of controllable narrow molecular weight functional chitosan oligosaccharide

[0103] Step 1: Media Preparation

[0104] (1) Chitosan medium preparation:

[0105] Described chitosan culture medium, by weight, raw material comprises:

[0106] 18 parts of chitosan, 380 parts of 1.5% acetic acid solution, (NH 4 ) 2 SO 4 5 parts of powder, 22 parts of skimmed milk powder, 800 parts of sterile water;

[0107] Dissolve chitosan in acetic acid solution, then add (NH 4 ) 2 SO 4 powder, skimmed milk powder and sterile water to prepare chitosan medium.

[0108] (2) Preparation of strain fermentation medium:

[0109] The strain fermentation medium, in parts by weight, raw materials include:

[0110] 0.8 parts of soybean flour, 0.04 parts of red algae culture solution, 1.6 parts of rice straw extract, 0.20 parts of corn flour, 0.8 parts of egg white liquid, 0.1 part of pork bone broth, 0.4 parts of calf serum, 0.07 parts o...

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Abstract

The invention provides a biological enzymolysis production method for controllable narrow molecular weight functional chitosan oligosaccharide. The biological enzymolysis production method includes the step of preparing a culture medium. The method further comprises the step of preparing a chitosan culture medium. The chitosan culture medium is prepared from, by weight, 15-18 parts of chitosan, 300-380 parts of acetic acid solution of 1.3-1.5%, 3-5 parts of (NH4)2SO4 powder, 20-22 parts of dried skim milk and 500-800 parts of sterile water. The effective content of chitosan oligosaccharide prepared through the method is 92.21-98.45%, and the deacetylation degree of the chitosan oligosaccharide prepared through the method is 95.6-98.2%; chitosan oligosaccharide is mainly used for preventing and controlling diseases in high-end agriculture and improving human immunity and used for preventing and controlling tumors and immunologic derangement diseases.

Description

technical field [0001] The invention relates to a method for producing chitosan oligosaccharide, in particular to a biological enzymolysis production method of controllable narrow molecular weight functional chitosan oligosaccharide. Background technique [0002] The content of chitin in nature is second only to cellulose, and it is mainly divided into the following three levels of derivative products based on its different processing methods: 1. Chitin (chitin), with a molecular weight of more than 1 million, suitable for industry and environmental protection Should be shipped, insoluble in water, it is difficult to be absorbed by the body. The molecular weight of chitosan is about 100,000, generally only soluble in organic weak acids, insoluble in water, can be absorbed by the human body, and can be used as food additives and raw materials for health products. Oligochitosan is an oligosaccharide with 2-10 glucosamines bound to β-1 and 4 positions. Its molecular weight is ...

Claims

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Application Information

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IPC IPC(8): C12P19/26C12P19/14
CPCC12P19/14C12P19/26
Inventor 刘维华张国臣
Owner 潍坊华诺生物科技有限公司
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