Tissue Culture and Rapid Breeding Method of Pig Bloodwood
A tissue culture, porcine blood wood technology, applied in the field of plant reproduction, can solve problems such as population difficulties and the reduction of wild resources of porcine blood wood, and achieve the effect of promoting division and differentiation, solving the problem of increasing reduction and small genetic variation.
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Embodiment 1
[0051] A rapid propagation method of pig blood wood tissue culture, comprising the following steps:
[0052] A. Cut out the new shoots of the old-fashioned pig blood tree, take the 5cm branches at the top, soak the branches with a 2% aqueous solution of detergent solution for 20 minutes, take them out, rinse them with linear tap water for 15 minutes, and then put them into natural water. Soak in the disinfectant solution for 30min, finally the branch is rinsed 3 times with sterile water and removes its surface moisture with sterile filter paper to obtain the explant, wherein,
[0053] The preparation method of the natural disinfectant is as follows: 10 parts by weight of garlic, 7 parts by weight of lemongrass, 6 parts by weight of Daidai, 6 parts by weight of Melaleuca mellifera, 5 parts by weight of Eucalyptus blue and 3 parts by weight of cloves are crushed to 50 mesh, Add 500 parts by weight of water to soak at room temperature for 1 hour, then vacuum reflux extraction at ...
Embodiment 2
[0076] A rapid propagation method of pig blood wood tissue culture, comprising the following steps:
[0077] A. Cut off the new shoots of the old-fashioned pig blood tree, take the 8cm branches at the top, soak the branches with a 2% aqueous solution of detergent for 20 minutes, take them out, rinse them with linear tap water for 18 minutes, and then put them into natural water. Soak in the disinfectant solution for 30min, finally the branch is rinsed 4 times with sterile water and removes its surface moisture with sterile filter paper to obtain the explant, wherein,
[0078] The preparation method of the natural disinfectant is: crush 11 parts by weight of garlic, 8 parts by weight of lemongrass, 7 parts by weight of Daidai, 7 parts by weight of Melaleuca mellifera, 6 parts by weight of Eucalyptus blue and 4 parts by weight of cloves to 80 mesh, Add 500 parts by weight of water to soak at room temperature for 1 hour, then vacuum reflux at 60°C for 3 hours, filter, and the obt...
Embodiment 3
[0101] A rapid propagation method of pig blood wood tissue culture, comprising the following steps:
[0102] A. Cut out the new shoots of the hemogonia in the same year, take the 10cm branches at the top, soak the branches with a 2% aqueous solution of detergent for 20 minutes, take them out, rinse them with linear tap water for 20 minutes, and then put them into natural water. Soak in the disinfectant solution for 30min, finally the branch is rinsed 5 times with sterile water and removes its surface moisture with sterile filter paper to obtain the explant, wherein,
[0103] The preparation method of the natural disinfectant is as follows: 12 parts by weight of garlic, 9 parts by weight of lemongrass, 8 parts by weight of Daidai, 8 parts by weight of Melaleuca mellifera, 7 parts by weight of Eucalyptus blue and 5 parts by weight of cloves are crushed to 100 mesh, Add 500 parts by weight of water to soak at room temperature for 1 hour, then vacuum reflux extraction at 60°C for ...
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Abstract
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Application Information
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