A dual vaccine against parvovirus in muscovy ducks and gosling plague
A Muscovy duck parvovirus and dual inactivated vaccine technology, which is applied in the direction of viruses, vaccines, antiviral agents, etc., can solve the problems of vaccine titer reduction, achieve good immunogenicity, good safety, and prevent young Muscovy ducks Effects of Parvovirus and Gosling Plague Virus Infection
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Embodiment 1
[0017] Example 1 Muscovy duck parvovirus seedling strain (YBMDP) screening
[0018] 1. Screening of YBMDP strain In 2011, an acute muscovy duck parvovirus disease broke out from a duck farm in Zhejiang Province. The pancreas and intestines of dead ducks were taken, and the pancreas and intestinal tissues were homogenized with non-normal saline to make 20 % suspension, centrifuged at 3000r / min for 15min, took the cleared bacteria and inoculated 13-day-old muscovy duck embryos through the allantoic cavity respectively, hatched for 120 hours, collected allantoic fluid and embryo body tissue, after homogenization, repeated freezing and thawing for 3 The second time, the supernatant was taken and frozen. After the harvested virus liquid was purified, the virus characteristics of the virus content, immunogenicity, specificity and purity were analyzed and tested, and the results showed that the virus content of the strain was 10 5.7 ELD 50 / 0.2ml, the minimum immune dose is 10 2.0...
Embodiment 2
[0027] Example 2 Preparation of vaccine
[0028] 1. Preparation of virus liquid for seedling production
[0029] 1.1 Preparation of virus liquid of YBMDP strain Dilute the YBMDP strain, the virus species used for production, 100 times with sterile saline, inoculate 11-12 day-old susceptible muscovy duck embryos in the allantoic cavity, 0.2ml per embryo, incubate at 37°C, 2 The next step is to check the embryo. Select duck embryos that died within 48 to 168 hours after inoculation, place them at 2 to 8°C for 4 to 12 hours, open the air chamber under sterile conditions, and pick out embryos with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and chorioallantois For embryos with mild edema, take the embryo body (remove the head and limbs) and put it into a tissue grinder to crush, collect allantoic fluid and amniotic fluid, homogenate with embryo fluid, take the crushed tissue and add it in a ratio of 1:1 Physiological saline was mixed, freeze-...
Embodiment 3
[0054] 1. Preparation of antigens for seedling production
[0055] 1. Preparation of virus liquid for seedling production
[0056] 1.1 Dilute the YBMDP strain, the virus species used for production, 100 times with sterile saline, inoculate 11-12 day-old susceptible muscovy duck embryos into the allantoic cavity, 0.2ml per embryo, incubate at 37°C, and inspect the embryos twice a day. Select duck embryos that died within 48 to 168 hours after inoculation, place them at 2 to 8°C for 4 to 12 hours, open the air chamber under sterile conditions, and pick out embryos with extensive hemorrhage, hair follicle hemorrhage, liver degeneration and necrosis, and chorioallantois For embryos with mild edema, take the embryo body (remove the head and limbs) and put it into a tissue grinder to crush, collect allantoic fluid and amniotic fluid, homogenate with embryo fluid, take the crushed tissue and add it in a ratio of 1:1 Physiological saline was mixed, freeze-thawed 3 times, centrifuged ...
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