Preparation method of skim squid protein functional active peptide with small molecular weight

A low-molecular-weight, active peptide technology, applied in the preparation methods of peptides, chemical instruments and methods, animal/human proteins, etc., can solve the problems of large protein molecular weight and waste of resources, and achieve a broad market and application prospects. , rich taste effect

Active Publication Date: 2016-09-07
舟山赛莱特海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are many ways to obtain active peptides from squid, but there are many shortcomings. Although high pressure is con

Method used

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  • Preparation method of skim squid protein functional active peptide with small molecular weight

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preparation example Construction

[0024] A preparation method of low molecular weight defatted squid protein functional active peptide, comprising the following steps in sequence:

[0025] Step 1. Pretreatment: Select Argentine squid or Peruvian squid with a thickness of 3cm to 8cm, remove the liver and other organs, and take out the spine and white mucous membrane similar to plastic sheets in the stomach to obtain squid board; and remove the squid board. Cut the leather surface with a razor blade, the blade cuts into about 1 / 2 of the thickness of the squid board, and the distance between each knife is 2cm to 5cm. Wash the squid board with pure water and grind it to get minced squid pieces or minced squid . Since the squid board is wrapped with squid skin, even when it is ground, there are relatively thick squid pieces with skin and flesh sticking together, so the cutting process is performed in advance to make the decolorization more uniform and rapid in the later stage.

[0026] Among them, the squid used i...

Embodiment 1

[0047] In this embodiment, the preparation method comprises the following steps in turn:

[0048] (1) Pretreatment: select Argentine squid or Peruvian squid with a thickness of 3 cm, remove the liver and other organs to obtain a squid board; The knives are separated by 5 cm, and the squid plate is cleaned with pure water and ground to obtain ground squid pieces.

[0049] (2) Decolorization: use 0.045mol / L NaOH solution as the decolorizing agent, according to the mass ratio of the squid pieces to the decolorizing agent is 100:4, add the decolorizing agent to the squid pieces and ultrasonically treat them, the temperature is 45°C, the power 300W, time 2h, washed with pure water until the pH is neutral to obtain a decolorized product.

[0050] (3) Color fixation: using Ca(OH) with a concentration of 0.4% (mass) 2 The solution is a color-fixing agent, the mass ratio of squid pieces to color-fixing agent is 100:3, the color-fixing agent is added to the decolorized product and tre...

Embodiment 2

[0057] In this embodiment, the preparation method comprises the following steps in turn:

[0058] (1) Pretreatment: select Argentine squid or Peruvian squid with a thickness of 5 cm, remove the liver and other organs to obtain squid boards; The knives are separated by 4 cm, and the squid board is cleaned with pure water and ground to obtain ground squid pieces.

[0059] (2) Decolorization: Use 0.025mol / L NaOH solution as the decolorizing agent, according to the mass ratio of the squid pieces to the decolorizing agent as 100:5, add the decolorizing agent into the squid pieces and ultrasonically treat them, the temperature is 55°C, the power 300W, time 1h, washed with pure water until the pH is neutral to obtain a decolorized product.

[0060] (3) Color fixation: using Ca(OH) with a concentration of 0.4% (mass) 2 The solution is a color-fixing agent. The mass ratio of squid pieces to color-fixing agent is 100:4. The color-fixing agent is added to the decolorized product and tr...

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Abstract

The invention relates to the field of nutrient supplements, in particular to a preparation method of a skim squid protein functional active peptide with small molecular weight. The preparation method comprises the following steps: firstly, performing viscera removal treatment on a squid to obtain a squid body and then performing cutting treatment and breaking treatment to obtain minced squid pieces; secondly, performing ultrasonic decoloring treatment, ultrasonic color fixing treatment and extraction treatment on the squid pieces to obtain a squid protein extracting solution; thirdly, performing enzymolysis treatment, pressurizing treatment and ultrafiltration treatment in sequence to obtain the active peptide. According to the preparation method provided by the invention, through pressurizing an enzymatic hydrolysate, the obtained active squid small peptide is uniform in broken chain, and the molecular weight can be controlled within 3KD; through the decoloring treatment and the color fixing treatment, the treatment time of the squid body is greatly shortened, and a fat layer in the squid body is removed, so that the quality of the small peptide is radically ensured; all steps and parameters in the preparation method provided by the invention have synergetic effects, so that the quality of an active peptide product can be further ensured.

Description

technical field [0001] The invention relates to the field of nutrient supplements, in particular to a preparation method of a low-molecular-weight defatted squid protein functional active peptide. Background technique [0002] With the development of my country's pelagic squid fishing industry, squid has become the main raw material for aquatic products processing in my country. According to reports, the annual processing capacity of squid in my country is about 300,000 to 400,000 tons of squid. Squid is rich in calcium, phosphorus, and iron, which is beneficial to bone development and hematopoiesis, and can effectively treat anemia; at the same time, it contains a large amount of taurine, which can inhibit the cholesterol content in the blood, relieve fatigue, restore vision, and improve liver function. It has anti-virus and anti-ray effects with selenium, and has the functions of nourishing yin and nourishing stomach, nourishing deficiency and moisturizing skin. [0003]...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K14/78C07K1/34
CPCC07K14/78C12P21/06
Inventor 宋文东郭健蔡璐纪丽丽
Owner 舟山赛莱特海洋科技有限公司
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