Bacillus subtilis K13, and culture method and application thereof
A technology of Bacillus subtilis and a culture method, applied in the field of Bacillus subtilis K13 and its culture, can solve the problem that the huge role of disease resistance gene resources has not been fully exerted, the situation of disease resistance rubber germplasm resources is unclear, and monitoring and early warning work technology Insufficient support and other problems, to achieve the effect of good development and application prospects, fast reproduction, and easy industrial production
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0039] Embodiment 1 Bacillus subtilis (Bacillus subtilis) K13
[0040]1. Sampling: Pick the leaves with anthracnose spots from the rubber tree, wash off the dust and gravel on the leaves with water, dry them, and use scissors to cut small diseased leaves at the junction of disease and health for later use; cut the size of the small diseased leaves It is 5mm×5mm.
[0041] 2. Sample disinfection: the small diseased leaves were put into a beaker, soaked with mercuric chloride for 30 seconds, stirred gently constantly, then rinsed three times with sterile water, and dried to obtain the small diseased leaves after disinfection.
[0042] 3. Isolation and cultivation of Bacillus subtilis (Bacillus subtilis) K13: put the sterilized small diseased leaves on the potato dextrose agar solid (PDA) medium with sterilized tweezers, place 4 pieces evenly in each dish, and then put Seal the petri dish with plastic wrap, put it upside down in a constant temperature incubator, cultivate it at 2...
Embodiment 2
[0053] Physiological and biochemical identification of embodiment 2 Bacillus subtilis (Bacillus subtilis) K13
[0054] 1. V-P test
[0055] 1) Principle
[0056] In the process of sugar metabolism, some bacteria decompose glucose to produce pyruvate, and decarboxylate pyruvate to produce acetylmethylmethanol. Acetylmethylmethanol is oxidized to diacetyl by oxygen in the air in an alkaline environment, and then reacts with peptone in the medium The guanidine group contained in arginine acts to generate a red compound, which means that the V-P test is positive. If the content of guanidinium in the medium is low, a small amount of guanidinium-containing compounds such as creatine or creatinine can be added. This reaction can be accelerated by adding α-naphthol during the test.
[0057] 2) Medium
[0058] Peptone 5.0g, Glucose 5.0g, K 2 HPO 4 (or NaCl) 5.0 grams, 1000 milliliters of distilled water, pH 7.0-7.2, divided into test tubes, 5 milliliters per test tube, sterilized...
Embodiment 5
[0095] Drawing of Growth Curve of Bacillus subtilis K13 Strain
[0096] 1) method
[0097]Determination by colorimetry: put liquid LB medium into a 2000ml Erlenmeyer flask, sterilize and cool, insert activated Bacillus subtilis (Bacillus subtilis) K13 strain, place on a shaker at 28°C and 180 rpm nourish. Samples were taken every two hours, and the absorbance (OD600) of the bacterial solution at 600 nm was measured.
[0098] 2) Results:
[0099] figure 2 It is the growth curve graph of Bacillus subtilis (Bacillus subtilis) K13 strain, wherein OD600 is the ordinate, and the culture time is the abscissa. Because the absorbance is determined by the density of the bacterial solution, the growth trend of the bacteria can be inferred from the change trend of the absorbance.
[0100] Depend on figure 2 It can be seen that: 0~7h, Bacillus subtilis (Bacillus subtilis) K13 bacterial strain grows slowly, and is in stagnant growth phase; 7~16h, Bacillus subtilis (Bacillus subtilis...
PUM
Property | Measurement | Unit |
---|---|---|
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com