Preparation method and application of a kind of kiwi fruit waste anti-tumor extract
An anti-tumor and extract technology, applied in the field of microbial utilization, can solve the problems of low content of active ingredients, low extraction efficiency and high equipment requirements, and achieve the effects of good anti-tumor activity, good extraction effect and high product purity.
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Embodiment 1
[0026] Prepare the anti-tumor extract as follows:
[0027] (1) Select the close relative species of Actinidia jujube, i.e. Actinidia purple fruit, and its discarded stems, leaves and fruits are crushed into granules not greater than 3mm, and the mixture of 1kg of the above-mentioned granules is taken, and Aspergillus niger ( The preservation number is CICC 40273), the humidity is adjusted to 8%, and the pH is 6.5. After mixing, fermentation is carried out in a koji box, and the fermentation temperature is controlled at 28°C for 48 hours;
[0028] (2) Add acetone with a volume content of 85% to the fermented product of step (1), the weight ratio of the added amount of acetone to the fermented product is 2:1, fully stir, and extract twice at 50° C. The time of secondary extraction is 2h;
[0029] (3) Filter after the leaching is finished, carry out vacuum distillation to remove acetone from the obtained filtrate, and cool the residue to room temperature to obtain 40 g of the pr...
Embodiment 2
[0033] Prepare the anti-tumor extract as follows:
[0034] (1) Get 200 g of pomace after squeezing the juice from the purple kiwifruit fruit, add Aspergillus niger (preservation number is CICC40273) to it, adjust the humidity to 4%, and the pH to 6.2. After mixing, use the stacking method to ferment and control the fermentation The temperature is 26°C, ferment for 60h;
[0035] (2) Add acetone with a volume content of 90% to the fermented product of step (1), the weight ratio of the added amount of acetone to the fermented product is 3:1, fully stir, and extract 3 times at 45° C. The time of secondary extraction is 2h;
[0036] (3) Filtrate after the leaching is finished, carry out vacuum distillation to remove acetone from the obtained filtrate, and cool the residue to room temperature to obtain 4 g of the precipitated product;
[0037] (4) Dissolve 4g of the precipitated product in 400mL of ethanol solution with a volume content of 90%, stir evenly, add sherwood oil therei...
Embodiment 3
[0040] Prepare the anti-tumor extract as follows:
[0041] (1) The leaves of the Actinidia jujube collected are crushed into crumbs not greater than 3mm, 500g of crumbs are taken, and Aspergillus niger (preservation number is CICC 40273) is added thereto, the humidity is adjusted to 1%, and the pH is 6.0, mixed with After homogeneity, use koji boxes to ferment, control the fermentation temperature to 25°C, and ferment for 96 hours;
[0042] (2) Add acetone with a volume content of 80% to the fermented product of step (1), the weight ratio of the added amount of acetone to the fermented product is 1.5:1, fully stir, and extract 3 times at 48° C. The time of secondary extraction is 3h;
[0043] (3) Filtrate after the leaching is finished, carry out vacuum distillation to remove acetone from the obtained filtrate, and cool the residue to room temperature to obtain 15 g of the precipitated product;
[0044] (4) Dissolve 15 g of the precipitated product in 1 L of ethanol solution...
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