Simultaneous extraction method of radix polygonati officinalis polysaccharide and oyster mushroom polysaccharide
The technology of Polysaccharide Polysaccharide and Mushroom Polysaccharide is applied in pharmaceutical formulations, preparations for skin care, cosmetics, etc., to achieve the effects of simple operation, strong water absorption capacity and antioxidant capacity, and enhanced moisturizing effect.
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Embodiment 1
[0017] Polygonatum and oyster mushrooms were mixed at a mass ratio of 1:1 (polygonatum and oyster mushrooms in total 100g) for rough extraction and concentrated to 50mL. At room temperature, 20mL of a two-phase aqueous system was prepared at a mass percentage of sodium carbonate and ethanol of 10%. The concentrated crude Add 5mL of the extract to the two-phase aqueous system, stir magnetically at a constant temperature (10°C, 300r / min) for 40min to enrich Polygonatum polysaccharide and Pleurotus ostreatus polysaccharide, let it stand for 80min, and centrifuge at 1500rpm for 30min to separate the enriched phase of the polysaccharide mixture , the polysaccharide solution extracted from the two-phase aqueous phase was placed in a 4°C refrigerator for a period of time, crystallized and precipitated most of the inorganic salts, and then dialyzed to obtain a mixed extract of polygonatum polysaccharide and oyster mushroom polysaccharide; take 10g of the extract, add flour, eggs, etc. t...
Embodiment 2
[0020] Polygonatum and Pyramid mushrooms were mixed at a mass ratio of 10:1 (Polygonatum and Pleurotus 100g in total) for rough extraction and concentrated to 50mL. At room temperature, 20mL of a two-phase system was prepared at a mass percentage of sodium carbonate and isopropanol of 30%. The concentrated Add 5mL of the crude extract to the two-phase system, stir magnetically at a constant temperature (35°C, 300r / min) for 30min to enrich Polygonatum Polysaccharides and Pleurotus polysaccharides, let it stand for 60min, and centrifuge at 1000rpm for 20min to separate the polysaccharide mixture rich in Phase collection, put the polysaccharide solution extracted from the two-phase aqueous phase in a refrigerator at 4 °C for a period of time, crystallize and precipitate most of the inorganic salts, and then dialyze to obtain a mixed extract of polygonatum polysaccharide and oyster mushroom polysaccharide; take 10g of the extract, add flour, eggs, etc. Make it into a paste; after w...
Embodiment 3
[0023] Mix Polygonatum and Pleurotus in a mass ratio of 4:1 (Polygonatum and Pleurotus 100g) for rough extraction and concentrate to 50mL. At room temperature, prepare 20mL of a two-phase system according to the mass percentage of ammonium sulfate and isopropanol at 30%. Add 5mL of the crude extract from the two-phase system to the two-phase system, stir magnetically at a constant temperature (50°C, 300r / min) for 60min to enrich Polygonatum polysaccharide and Pleurotus ostreatus polysaccharide, let it stand for 100min, and centrifuge at 2000rpm for 40min to separate the polysaccharide mixture rich in Phase collection, put the polysaccharide solution extracted from the two-phase aqueous phase in a refrigerator at 4 °C for a period of time, crystallize and precipitate most of the inorganic salts, and then dialyze to obtain a mixed extract of polygonatum polysaccharide and oyster mushroom polysaccharide; take 10g of the extract, add flour, eggs, etc. Make it into a paste; after wa...
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