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Synthetic method of aldehyde dextran, aldehyde dextran-based coating method, and preparation method of microsphere composition

An aldehyde-based glucan and a synthesis method technology are applied in the preparation of microsphere compositions and the synthesis field of aldehyde-based glucan, and can solve the problems of difficult control of conditions, interference with detection results and the like, and achieve controllable aldehyde-based density, The effect of simple coating process and short cycle

Active Publication Date: 2016-09-21
成都爱兴生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because of the addition of an essential activation process, this reaction is more prone to coalescence of particles than the aldehyde-based one-step reaction, and the conditions are not easy to control
[0005] 2. Except for the carboxyl groups, the surface of the microspheres is a bare hydrophobic surface, and these hydrophobic surfaces are prone to non-specific adsorption during the binding process of proteins and microspheres, and are more susceptible to solvents, pH and even salt concentration. influence, thereby interfering with the test results

Method used

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  • Synthetic method of aldehyde dextran, aldehyde dextran-based coating method, and preparation method of microsphere composition
  • Synthetic method of aldehyde dextran, aldehyde dextran-based coating method, and preparation method of microsphere composition

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Embodiment 1

[0093] A synthetic method of aldehyde dextran, comprising the following steps:

[0094] A. After dissolving 30g of dextran in 90ml of water, under nitrogen protection, add 10mg of EDTA, 10mg of 1,4-benzenediol, 10mg of sodium borohydride, 6g of NaOH and 60ml of toluene, and stir and reflux for 2 hours;

[0095] B. Slowly add the toluene solution of 2-bromo-1,1-diethoxyethane dropwise to the system, and the dropping time is not less than 1 hour, the 2-bromo-1,1-diethoxyethane The toluene solution is prepared by dissolving 50ml of 2-bromo-1,1-diethoxyethane in 200ml of toluene solution; after the dropwise addition, stir and reflux for 15 hours;

[0096] C, after being cooled to room temperature, wash the organic phase in the system with water three times, the volume of each water used is 150ml, merge the aqueous phase to obtain an aqueous solution, the aqueous solution is added in the methanol of 2000ml, wash three times with ethanol after suction filtration, each The volume of...

Embodiment 2

[0099] A synthetic method of aldehyde dextran, comprising the following steps:

[0100] A. After dissolving 20g of dextran in 130ml of water, under nitrogen protection, add 5mg of EDTA, 15mg of 1,4-benzenediol, 5mg of sodium borohydride, 8g of NaOH and 40ml of toluene, and stir and reflux for 3 hours;

[0101]B. Slowly add the toluene solution of 2-bromo-1,1-diethoxyethane dropwise to the system, and the dropping time is not less than 1 hour, the 2-bromo-1,1-diethoxyethane The toluene solution is prepared by dissolving 40ml of 2-bromo-1,1-diethoxyethane in 200ml of toluene solution; after the dropwise addition is completed, stir and reflux for 12 hours;

[0102] C, after being cooled to room temperature, wash the organic phase in the system with water three times, the volume of each water used is 180ml, merge the aqueous phase to obtain an aqueous solution, the aqueous solution is added in the methanol of 1500ml, wash three times with ethanol after suction filtration, each Th...

Embodiment 3

[0105] A synthetic method of aldehyde dextran, comprising the following steps:

[0106] A. After dissolving 40g of dextran in 50ml of water, under nitrogen protection, add 15mg of EDTA, 5mg of 1,4-benzenediol, 15mg of sodium borohydride, 4g of NaOH and 80ml of toluene, and stir and reflux for 1 hour;

[0107] B. Slowly add the toluene solution of 2-bromo-1,1-diethoxyethane dropwise to the system, and the dropping time is not less than 1 hour, the 2-bromo-1,1-diethoxyethane The toluene solution is prepared by dissolving 45ml of 2-bromo-1,1-diethoxyethane in 200ml of toluene solution; after the dropwise addition is completed, stir and reflux for 18 hours;

[0108] C, after being cooled to room temperature, wash the organic phase in the system with water three times, the volume of each water used is 120ml, merge the aqueous phase to obtain an aqueous solution, the aqueous solution is added in the methanol of 2500ml, wash three times with ethanol after suction filtration, each Th...

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Abstract

The invention relates to a synthetic method of aldehyde dextran, an aldehyde dextran-based coating method, and a preparation method of a microsphere composition. The aldehyde dextran is prepared from haloacetal and glucan through substitution and hydrolysis reactions, generated aldehyde groups are difficult to cross-link, the density of the active groups aldehyde groups is controllable, and the reappearance is good, so monoclonal antibody and protein connection is facilitated. The coating method is characterized in that epoxy ethyl particles are adopted as an initial raw material to substitute traditional carboxyl particles. Organic micromolecules can be used to substitute aminosugar, so the whole coating process is simple, and the coating period is short, so the coating method is suitable for industrial production. The preparation method of the microsphere composition is characterized in that the surfaces of microspheres are coated with the aldehyde dextran to form aldehyde dextran microspheres used for covalent coupling of biological molecules. The preparation method has the advantages of simple process, simple operation and good repeatability.

Description

technical field [0001] The invention belongs to the field of chemical synthesis, and in particular relates to a synthesis method of aldehyde dextran, a coating method based on the aldehyde dextran and a preparation method of a microsphere composition. Background technique [0002] Polystyrene microspheres have been widely used in the field of medical diagnosis. The main principle is that proteins can be covalently cross-linked to the surface of microspheres in various ways. The surface of the microspheres can be modified with functional groups such as epoxy groups, chloromethyl groups, aldehyde groups, and carboxyl groups, all of which can react with the amino groups at the Fc end of the antibody to form covalent bonds. When choosing a covalent cross-linking method, it is often necessary to consider the validity period of the cross-linking, the complexity of the process, and the final charge stability. The first three groups can directly react with the amino group of the pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02C08J7/12C08L25/06
CPCC08B37/0006C08J7/12C08J2325/06
Inventor 包德泉
Owner 成都爱兴生物科技有限公司
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