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Bioreactor for perfusion and bioreactor for permeation

A bioreactor and needle technology, which is applied in the field of bioreactors and permeable bioreactors, can solve the problems of fixed hollow circular tubes, ineffective sealing of hollow circular tubes, etc., and achieves convenient operation, simple structure and easy realization. Effect

Active Publication Date: 2016-09-21
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sodium alginate solution and calcium chloride solution can be extruded through the hollow coaxial needle to form a hollow tube in tissue engineering. The structure maintenance performance and permeability of the hollow tube are two characteristics that are of great concern in the engineering of the group. For research The ability of the hollow blood vessel network to maintain the structure of the tube is necessary for perfusion experiments and infiltration experiments. However, the existing technology cannot fix the position of the hollow tube well, and cannot effectively seal the gap between the hollow tube and the needle. voids, so it cannot provide better environmental conditions for perfusion experiments and infiltration experiments

Method used

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  • Bioreactor for perfusion and bioreactor for permeation
  • Bioreactor for perfusion and bioreactor for permeation
  • Bioreactor for perfusion and bioreactor for permeation

Examples

Experimental program
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Effect test

Embodiment 1

[0025] The ability of the printed hollow tube to transport cell culture fluid and maintain the structure of the tube was studied through a perfusion system, which includes a peristaltic pump, a bioreactor for perfusion, a liquid storage bottle, and a catheter. Bioreactors for perfusion such as figure 1 As shown, the third groove 8 is used as the perfusion chamber, and the second groove 7 and the fourth groove 9 are used as the sealing chambers on the left and right sides. After printing out the hollow tube, cut out a hollow tube of appropriate length and Connect the liquid inlet needle 1 and the liquid outlet needle 2 at its two ends, then place it in the housing, inject 20% gelatin (TG enzyme concentration is 1.2%, let it stand for 10-20s until the gelatin is gelled) into the sealed cavity, and finally, Connect the liquid inlet needle 1 and the liquid outlet needle 2 into the perfusion system to complete the perfusion culture.

Embodiment 2

[0027] The bioreactor for permeation according to the present invention comprises a sealing cover plate 14 and a bioreactor for perfusion, wherein the third groove 8 in the bioreactor for perfusion is sealed by the sealing cover plate 14 , cells are mixed in the gelatin in the third groove 8 .

[0028] The ability of the printed hollow tube to infiltrate the cell culture medium was studied through the infiltration experiment, and the infiltration system included a peristaltic pump, a bioreactor for infiltration, a liquid storage bottle and a catheter. Bioreactors for osmosis such as Figure 4 As shown, the third groove 8 is used as the middle permeation chamber, and the second groove 7 and the fourth groove 9 are used as the sealing chambers on the left and right sides. After printing out the hollow tube, cut out a hollow tube of appropriate length And connect the liquid inlet needle 1 and the liquid outlet needle 2 at both ends, then fix it in the casing, and then inject 20%...

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Abstract

The invention discloses a bioreactor for perfusion and a bioreactor for permeation. The bioreactor for perfusion comprises a liquid inlet needle head, a liquid outlet needle head, a hollow round tube, a shell, a first retaining plate and a second retaining plate; the upper surface of the shell is provided with a first groove which is divided into a second groove, a third groove and a fourth groove sequentially through the first retaining plate and the second retaining plate, the left end of the hollow round tube penetrates the first retaining plate to extend into the second groove, the right end of the hollow round tube penetrates the second retaining plate to extend into the fourth groove, the middle of the hollow round tube is located in the third groove, the end of the liquid inlet needle head penetrates the wall surface of the shell to extend into the second groove, the end of the liquid inlet needle head is connected into the left end of the hollow round tube in a sleeve manner, the end of the liquid outlet needle head penetrates the wall surface of the shell to extend into the fourth groove, and the end of the liquid outlet needle head is connected into the right end of the hollow round tube in a sleeve manner. The hollow round tube can be fixed in position, and the clearances between the hollow round tube and the needle heads can be sealed effectively.

Description

technical field [0001] The invention belongs to the field of mechanical automation and biotechnology, relates to a bioreactor, in particular to a bioreactor for perfusion and a bioreactor for permeation. Background technique [0002] Tissue engineering simulates the natural tissue structure and uses three elements such as cells, scaffolds, and growth factors to construct artificial tissues for the repair of damaged or degenerated tissues. Among them, the scaffold is the key to the spatial transportation and positioning of cells and growth factors, and has been successfully applied to artificial skin, bone, cartilage, cartilage and bladder and other organs. However, for artificial tissues and organs with large cell mass, high oxygen consumption, such as muscle, heart, liver, and kidney, and high requirements for the exchange of nutrients, oxygen, and metabolic waste, it is difficult to meet the demand for vascularization, that is, it is difficult to manufacture And form a va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/00
CPCC12M21/08C12M25/10C12M37/04
Inventor 连芩毛伟李涤尘郭启帆贺健康
Owner XI AN JIAOTONG UNIV