A method based on pulsed electric field and carbon nanotubes synergistically driving Saccharomyces cerevisiae cell wall breaking

A technology of Saccharomyces cerevisiae cells and carbon nanotubes, which is applied in the fields of biochemical equipment and methods, dissolution of microorganisms, and electric/wave energy treatment of enzymes, etc. problem, to achieve the effect of simple operation process, improved extraction rate and low production cost
CN105950476BInactive Publication Date: 2019-12-10GUANGDONG UNIV OF TECH
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
GUANGDONG UNIV OF TECH
Publication Date
2019-12-10
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for brewer's yeast cell disruption based on collaborative driving of a pulsed electric field and carbon nano tubes. The method comprises the following steps that firstly, carbon nano tube-deionized dispersing solutions with the gradient of different concentrations are prepared; secondly, the dispersing solutions and brewer's yeast are mixed evenly, and carbon nano tube-yeast suspensions are obtained; thirdly, the carbon nano tube-yeast suspensions are put into a high-pressure pulse treatment chamber for cell disruption, and disrupted yeast cells are obtained; fourthly, the yeast cell disruption effect is detected through extraction and measurement of nucleic acid. Under the same operation conditions, the cell disruption rate of the brewer's yeast is remarkably increased compared with that achieved in pure pulse electric field or carbon nano tube treatment. The method is simple in process, easy to implement, capable of achieving a good cell disruption effect in a short time and beneficial to improving extraction of nutrient substances such as intracellular nucleic acid of brewer's yeast.
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Description

technical field

[0001] The invention relates to a method for breaking the wall of Saccharomyces cerevisiae, in particular to a method for breaking the wall of Saccharomyces cerevisiae cells based on a pulsed electric field and carbon nanotubes, so as to achieve the purpose of extracting intracellular nucleic acid. Background technique

[0002] In the process of beer production, a large amount of yeast will be entrained. For every 100 tons of beer produced, there will be 1.5-2.0 tons of waste yeast. The beer industry in my country can produce 600,000-800,000 tons of waste yeast every year. At present, most of the waste beer yeast is used as fertilizer and feed, and the utilization rate is very low. Some enterprises directly discharge the waste yeast into beer wastewater as waste, which increases the processing load and difficulty of beer wastewater and causes a lot of waste. These waste yeast are rich in zymosan, ribonucleic acid, protein and other substances, which have impo...

Claims

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