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A method based on pulsed electric field and carbon nanotubes synergistically driving Saccharomyces cerevisiae cell wall breaking

A technology of Saccharomyces cerevisiae cells and carbon nanotubes, which is applied in the fields of biochemical equipment and methods, dissolution of microorganisms, and electric/wave energy treatment of enzymes, etc. problem, to achieve the effect of simple operation process, improved extraction rate and low production cost

Inactive Publication Date: 2019-12-10
GUANGDONG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] (1) The traditional physical wall-breaking method needs to consume a lot of energy, and the heat generated reduces the biological activity of intracellular substances such as proteins and nucleic acids
[0006] (2) The chemical treatment method can easily cause the denaturation of nutritional active substances, and the follow-up treatment is cumbersome
[0007] (3) biological wall breaking method, long reaction time, high cost, unfavorable for industrialized production
After searching, there is no report on the combination of ultrasonic pulsed electric field and carbon nanotubes acting on yeast cells to break the wall

Method used

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  • A method based on pulsed electric field and carbon nanotubes synergistically driving Saccharomyces cerevisiae cell wall breaking
  • A method based on pulsed electric field and carbon nanotubes synergistically driving Saccharomyces cerevisiae cell wall breaking

Examples

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Embodiment 1

[0033] The method for breaking the cell wall of Saccharomyces cerevisiae cells based on a pulsed electric field and carbon nanotubes in this embodiment is as follows: 1 g of multi-walled carbon nanotubes is dispersed uniformly in 1000 mL of deionized water by ultrasonic, and the ultrasonic time is 0.5 h. The gradient is 1g / L carbon nanotube-deionized dispersion. Put 1g of beer dry yeast in the ratio of 1:10 in warm water at 35°C for 15 minutes to make a yeast suspension, add 30mL of 1g / L carbon nanotube-deionized dispersion, and stir mechanically for 0.5h to obtain carbon nanotubes Tube - yeast suspension. The prepared carbon nanotube-yeast suspension was placed in a treatment chamber between two plate-shaped iron electrodes, and the distance between the electrodes was kept at 40 cm. The pulse discharge voltage is 10kV, the frequency is 50Hz, the pulse width is 40μs, the number of pulses is 500, and the pulse electric field treatment time is 1s, and the broken yeast cells are...

Embodiment 2

[0034] Example 2: A method for synergistically driving Saccharomyces cerevisiae cell wall breaking by a pulsed electric field and carbon nanotubes, the steps are as follows: 1 g of multi-walled carbon nanotubes is dispersed uniformly in 500 mL of deionized water by ultrasonic, and the ultrasonic time is 0.5 h, configured as The concentration gradient is 2g / L carbon nanotube-deionized dispersion. Put 1.5g of beer dry yeast in the ratio of 1:15 in warm water at 36°C and rehydrate for 18 minutes to make a yeast suspension, add 30mL 2g / L carbon nanotube-deionized dispersion, and stir mechanically for 0.5h to obtain carbon nanotubes Tube - yeast suspension. The prepared carbon nanotube-yeast suspension was placed in a treatment chamber between two plate-shaped iron electrodes, and the distance between the plate electrodes was kept at 20 cm. The pulse discharge voltage is 20kV, the frequency is 100Hz, the pulse width is 60μs, the pulse number is 800 times, and the pulse electric fi...

Embodiment 3

[0035]Example 3: A method for synergistically driving Saccharomyces cerevisiae cell wall breaking by a pulsed electric field and carbon nanotubes, the steps are as follows: 1 g of multi-walled carbon nanotubes is dispersed evenly in 200 mL of deionized water by ultrasonic, and the ultrasonic time is 0.5 h, configured as The concentration gradient is 5g / L carbon nanotube-deionized dispersion. Put 2g of beer dry yeast in the ratio of 1:20 in warm water at 38°C and rehydrate for 20 minutes to make a yeast suspension, add 30mL of 1g / L carbon nanotube-deionized dispersion, and stir mechanically for 0.5h to obtain carbon nanotubes - Yeast suspension. The prepared carbon nanotube-yeast suspension was placed in a treatment chamber between two plate-shaped iron electrodes, and the distance between the plate electrodes was kept at 10 cm. The pulse discharge voltage is 40kV, the frequency is 200Hz, the pulse width is 100μs, the pulse number is 1000 times, and the pulse electric field tr...

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Abstract

The invention discloses a method for brewer's yeast cell disruption based on collaborative driving of a pulsed electric field and carbon nano tubes. The method comprises the following steps that firstly, carbon nano tube-deionized dispersing solutions with the gradient of different concentrations are prepared; secondly, the dispersing solutions and brewer's yeast are mixed evenly, and carbon nano tube-yeast suspensions are obtained; thirdly, the carbon nano tube-yeast suspensions are put into a high-pressure pulse treatment chamber for cell disruption, and disrupted yeast cells are obtained; fourthly, the yeast cell disruption effect is detected through extraction and measurement of nucleic acid. Under the same operation conditions, the cell disruption rate of the brewer's yeast is remarkably increased compared with that achieved in pure pulse electric field or carbon nano tube treatment. The method is simple in process, easy to implement, capable of achieving a good cell disruption effect in a short time and beneficial to improving extraction of nutrient substances such as intracellular nucleic acid of brewer's yeast.

Description

technical field [0001] The invention relates to a method for breaking the wall of Saccharomyces cerevisiae, in particular to a method for breaking the wall of Saccharomyces cerevisiae cells based on a pulsed electric field and carbon nanotubes, so as to achieve the purpose of extracting intracellular nucleic acid. Background technique [0002] In the process of beer production, a large amount of yeast will be entrained. For every 100 tons of beer produced, there will be 1.5-2.0 tons of waste yeast. The beer industry in my country can produce 600,000-800,000 tons of waste yeast every year. At present, most of the waste beer yeast is used as fertilizer and feed, and the utilization rate is very low. Some enterprises directly discharge the waste yeast into beer wastewater as waste, which increases the processing load and difficulty of beer wastewater and causes a lot of waste. These waste yeast are rich in zymosan, ribonucleic acid, protein and other substances, which have impo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/06C12N13/00C12N15/10
CPCC12N1/063C12N1/066C12N13/00C12N15/1003
Inventor 刘丹丁利君肖思杰
Owner GUANGDONG UNIV OF TECH
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