Pantoea sp. for degrading polycyclic aromatic hydrocarbon organic pollutants

A technology for organic pollutants and polycyclic aromatic hydrocarbons, which is applied in the field of degrading Pantoea and polycyclic aromatic hydrocarbons organic pollutants. Pollution and other issues, to achieve the effect of wide temperature adaptability

Active Publication Date: 2016-09-21
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The traditional remediation of PAH pollution mainly relies on physical and chemical remediation (chemical oxidation, guest soil or soil upturning, stripping, thermal desorption, incineration, landfill and other methods to remove or fix pollutants in the soil), these methods Often the treatment cost is high, the repair is not thorough, easy to produce secondary pollution and not suitable for large-scale application

Method used

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  • Pantoea sp. for degrading polycyclic aromatic hydrocarbon organic pollutants
  • Pantoea sp. for degrading polycyclic aromatic hydrocarbon organic pollutants
  • Pantoea sp. for degrading polycyclic aromatic hydrocarbon organic pollutants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The isolation and identification of embodiment 1PYR16 bacterial strain

[0025] The long-term oil-contaminated soil from Changqing Oilfield was used as the screening soil. The specific screening scheme is as follows:

[0026] 1) Take 10g of soil, add it to the inorganic salt medium MSM containing 200mg / L pyrene, culture with shaking at 28°C for 15 days, and then receive the above-mentioned inorganic salt medium MSM containing 200mg / L pyrene with a 10% inoculation amount for cultivation Base center; transfer 5 times in a row.

[0027] 2) Take 0.1ml of the enriched culture solution obtained in step 1), spread it on the MSM solid plate containing pyrene, and after static culture at 28°C for 15 days, transfer the colony grown on the plate to the MSM solid plate containing pyrene On the plate, continue to grow until a single colony appears on the plate.

[0028] 3) Pick a single colony grown on a pyrene-containing MSM solid plate, inoculate it into an MSM liquid medium con...

Embodiment 2

[0032] Example 2 PYR16 bacterial strains to the degradation characteristics of pyrene

[0033] Using pyrene as the sole carbon source and using MSM medium as the base medium, the inventors tested the pyrene degradation ability of the PYR16 strain. First, the preserved strains were removed from -80°C and then inoculated into R 2 Activated in A solid medium for 1 day; pick a single colony and inoculate into 5 ml R 2 A liquid culture medium to OD 600 = 0.5; then take 0.5ml of bacterial liquid and inoculate into 25ml of MSM medium containing 200mg / L of pyrene, and culture with shaking at 28°C. In the above degradation system, the insertion amount of the PYR16 strain was about 10 5 -10 6 about. Take out a bottle of culture every 24 hours and measure the OD separately 600 value, and use acetone / n-hexane (1:1) to extract all residual pyrene in the Erlenmeyer flask, and then use GC-FID to analyze the pyrene content. Three bottles were taken each time for parallel analysis.

[00...

Embodiment 3

[0036] The influence of embodiment 3pH, sodium ion and temperature on pyrene degradation ability of PYR16 bacterial strain

[0037] First, the MSM medium was adjusted to a pH of 4, 5, 6, 7, 8, 9, 10 with hydrochloric acid or sodium hydroxide solution, and then inoculated according to the operation method of Example 2 for 10 5 -10 6 About the PYR16 strain, the concentration of residual pyrene in the culture system was analyzed after shaking culture at 28°C for 10 days. like image 3 As shown, in the pH range of 6-10, the PYR16 strain can maintain the degradation effect on pyrene, but when the pH is lower than 6, its degradation ability will be significantly inhibited. Therefore, the pH of the suitable treatment environment for the pyrene-degrading bacteria provided by the present invention should be adjusted to between 6-10, and when the pH of the treated sample is adjusted to 6-10, the PYR16 strain can maintain 34% of the pyrene substance. More than % degradation efficiency...

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Abstract

The invention discloses pantoea sp. PYR16 for degrading polycyclic aromatic hydrocarbon organic pollutants, and belongs to the field of microbial and environmental organic pollutant repair and control. The strain is preserved in China Center for Type Culture Collection, with preservation number of CCTCC NO: M2016227. The strain disclosed by the invention can be used for effectively degrading various polycyclic aromatic hydrocarbons; the strain is broad in environment-adaptive and heavy-metal resistant capacities; and the strain, when implanted in severe environments, can be used for repairing soil or water bodies which are affected by the combined pollution of heavy metals and polycyclic aromatic hydrocarbons.

Description

technical field [0001] The invention belongs to the field of remediation and treatment of microorganisms and environmental organic pollutants, and in particular relates to a strain of Pantoea sp. that degrades organic pollutants of polycyclic aromatic hydrocarbons. The invention also relates to the application of the Pantoea sp. and a method for degrading polycyclic aromatic hydrocarbons approach to organic pollutants. Background technique [0002] Polycyclic aromatic hydrocarbons (referred to as PAHs) refer to a class of condensed ring hydrocarbons arranged in a linear, angular or clustered form with two or more benzene rings, among which PAHs with 4 or more benzene rings are called High molecular weight polycyclic aromatic hydrocarbons (High molecular weight-PAHs, referred to as HMW-PAHs). The sources of polycyclic aromatic hydrocarbons mainly include two aspects: one is the activities of nature itself, such as forest fires, decay of animal and plant remains, formation of...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C02F3/34C12R1/01C02F101/20C02F101/32
CPCB09C1/10C02F3/34C02F2101/20C02F2101/327C12N1/205C12R2001/01
Inventor 黄巧云陈雯莉汪豪杰汪方奎李偲胡甜杨阳
Owner HUAZHONG AGRI UNIV
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