Method for producing donkey-hide gelatin small peptide by utilizing bionic enzymatic hydrolysis technology and application thereof

A technology of bionic enzymolysis and donkey-hide gelatin, applied in the biological field, can solve the problems of affecting the properties of small peptide products, large loss of product peptides, incomplete purification, etc., to improve human immunity, reduce the loss of active peptides, and increase the yield Effect

Inactive Publication Date: 2016-09-28
安徽精准医疗产业研究院有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In the existing process, due to incomplete refining and purification in the later stage, the loss of pro

Method used

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  • Method for producing donkey-hide gelatin small peptide by utilizing bionic enzymatic hydrolysis technology and application thereof
  • Method for producing donkey-hide gelatin small peptide by utilizing bionic enzymatic hydrolysis technology and application thereof
  • Method for producing donkey-hide gelatin small peptide by utilizing bionic enzymatic hydrolysis technology and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] The method for producing donkey-hide gelatin small peptides by biomimetic enzymolysis technology comprises the following steps:

[0038] (1) Grinding donkey-hide gelatin, passing through a 80-mesh sieve, adding 10 times the weight of water to homogenate, adding dilute hydrochloric acid, adjusting the pH to 1.5-2, adding pepsin with 1% of the mass of donkey-hide gelatin, and enzymatically hydrolyzing it at 40°C for 1 hour; Then, add sodium hydroxide solution to adjust the pH to 7.5-8, add trypsin with 1% donkey-hide gelatin mass, and enzymolyze at 40° C. for 3 hours to obtain an enzymatic hydrolysis solution;

[0039] (2) Centrifuge the enzymatic solution at 4000-4500r / min for 10-15min, and take the supernatant. , take the supernatant, perform ultrafiltration with a hollow fiber membrane with a molecular weight cut-off of 3KD, and collect the permeate; after testing, the total peptide amount before filtration is 19.24g; after filtration, the total peptide amount is 19.02...

Embodiment 2

[0047]The method for producing donkey-hide gelatin small peptides by biomimetic enzymolysis technology comprises the following steps:

[0048] (1) Grind donkey-hide gelatin, pass through a 100-mesh sieve, add 15 times the weight of water to homogenate, add dilute hydrochloric acid, adjust the pH to 1.5-2, add pepsin with 0.8% donkey-hide gelatin mass, and enzymatically hydrolyze at 38°C for 1.5h Then, add sodium hydroxide solution to adjust the pH to 7.5-8, add trypsin with 0.8% donkey-hide gelatin mass, and enzymolyze at 42° C. for 3 hours to obtain an enzymatic hydrolysis solution;

[0049] (2) Centrifuge the enzymatic solution at 4000-4500r / min for 10-15min, and take the supernatant. , take the supernatant, perform ultrafiltration with a hollow fiber membrane with a molecular weight cut-off of 3KD, and collect the permeate; after testing, the total peptide amount before filtration is 19.24g; after filtration, the total peptide amount is 19.02g, and the loss peptide amount i...

Embodiment 3

[0053] Application of biomimetic enzymolysis technology to produce donkey-hide gelatin small peptides, specifically compound peptide donkey-hide gelatin pulp, containing the following raw materials in parts by weight:

[0054]

[0055]

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PUM

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Abstract

The invention discloses a method for producing donkey-hide gelatin small peptide by utilizing the bionic enzymatic hydrolysis technology and application thereof. According to the method, the number of grinding and screening meshes, addition amount, enzymolysis temperature, pH, enzyme addition amount and other aspects are controlled and optimized, the addition amount of pepsin and trypsin is small, cost is reduced, bionic enzymatic hydrolysis is high in yield, the composition of pepsin and trypsin is subjected to bionic enzymatic hydrolysis, and the gelatin output is 1.5-2 times that of the traditional process. Furthermore, the product obtained by the bionic enzymatic hydrolysis technology is separated and purified by the technology of combining ultrafiltration membrane separation, electroosmosis and resin column chromatography, the loss amount of bioactive peptide is less than 2 percent, and the small peptide having a relative molecular mass of less than 2KD is more than 80 percent, so that the loss of bioactive peptide obtained by enzymolysis can be reduced, the small-molecular peptide content is high, and the using value is increased. The donkey-hide gelatin small peptide is matched with oligopeptides of marine fish, soybean oligopeptide, casein phosphopeptides and the like to obtain donkey-hide gelatin slurry, and can be used for improving human body immunity and relieving fatigue and senescence.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for producing donkey-hide gelatin small peptides using a biomimetic enzymolysis technology and an application thereof. Background technique [0002] Donkey-hide gelatin is a solid gelatin made from the skin of an equine animal donkey, which is boiled and concentrated. It was originally produced in the Pandong A region of Shandong Province and has a history of nearly 3,000 years. Donkey-hide gelatin is rich in protein. It is a traditional top-grade nourishing and blood-enriching medicine. It tastes sweet and flat. It enters the lungs, liver, and kidneys. It has the effects of nourishing blood and stopping bleeding, nourishing yin and moistening dryness. Nourishing, anti-aging, anti-fatigue, improving immunity, suitable for a wide range of people. [0003] The traditional nutrition view holds that protein nutrition is amino acid nutrition, and the need for protein...

Claims

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Application Information

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IPC IPC(8): A23L13/20A23L13/40A23L33/18A23L33/175A23L33/10A23L5/20
CPCA23V2002/00A23V2200/324A23V2200/302A23V2200/30A23V2250/55A23V2250/038A23V2250/0644A23V2250/616A23V2300/02A23V2300/10A23V2300/12
Inventor 张斌张士伟陶少强李玉峰
Owner 安徽精准医疗产业研究院有限公司
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