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Method for quickly detecting monosaccharide composition in medicine mulberry polysaccharide through UPLC-TUV

A technology of medicine mulberry and monosaccharide, which is applied in the field of rapid detection of polysaccharide components, can solve the problems of failing to meet the separation requirements and long time consumption, and achieve the effects of good accuracy, good specificity and high sensitivity

Inactive Publication Date: 2016-09-28
SOUTHWEST UNIVERSITY
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AI Technical Summary

Problems solved by technology

Due to the differences between the UPLC chromatographic column and the HPLC chromatographic column, as well as the difference in the composition of interfering components and monosaccharides, when referring to the HPLC-TUV method for other polysaccharides reported in the relevant literature, it takes too long, and specific types of monosaccharides also reach less than separation requirement

Method used

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  • Method for quickly detecting monosaccharide composition in medicine mulberry polysaccharide through UPLC-TUV
  • Method for quickly detecting monosaccharide composition in medicine mulberry polysaccharide through UPLC-TUV

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Embodiment 1

[0032] 1. Instruments and reagents

[0033] 1.1 Instrument

[0034] DHG-9140A electric constant temperature blast drying oven, Shanghai Qixin Scientific Instrument Co., Ltd.; GX-03 150g functional pulverizer, Zhejiang Gaoxin Industry and Trade Co., Ltd.; FA2004B electronic balance, Shanghai Jingtian Electronic Instrument Co., Ltd.; KQ-500DV CNC Ultrasonic cleaner, Kunshan Ultrasonic Instrument Co., Ltd.; CT15RE refrigerated centrifuge, Hitachi, Japan; Milli-Qsystem, Millipore, USA; Acquity UPLC I-Class system, Waters, USA.

[0035] 1.2 Reagents

[0036] D-anhydroglucose (>98%), D-mannose (>98%), D-galactose (>98%), D-xylose (>98%), D-glucuronic acid (>98%) ), L-rhamnose (>98%), and L-arabinose (>98%) were purchased from Chengdu Croma Biotechnology Co., Ltd.; chromatographic grade acetonitrile and methanol were purchased from U.S. Thermo Fisher Scientific; chromatographic grade acetic acid Ammonium, ammonium formate, and 1-phenyl-3-methyl-5-pyrazolinone (PMP) were purchased ...

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Abstract

The invention discloses a method for rapidly detecting the composition of monosaccharides in mulberry polysaccharides by using UPLC-TUV. (PMP) pre-column derivatization, and then enter the UPLC-TUV system for detection. The invention discloses detection conditions for ultra-high performance liquid chromatography detection. The method is fast, sensitive, good in separation effect and high in repeatability, and can quickly and accurately detect the composition of monosaccharides in the polysaccharide of mulberry fruit.

Description

technical field [0001] The invention belongs to the field of rapid detection of polysaccharide components, in particular to a method for rapidly detecting the composition of monosaccharides in mulberry polysaccharides by using UPLC-TUV. Background technique [0002] The black mulberry species of the genus Moraceae Morus originated in Iran. It was cultivated in Xinjiang and other places in my country in the 16th century. It is also mainly distributed in Aksu, Hotan, Turpan and Kashgar in Xinjiang. It is the only black mulberry species in my country. Medicinal mulberry is its ripe fruit, containing a variety of nutrients and medicinal ingredients. Phytochemical studies have confirmed that mulberry contains various active ingredients such as alkaloids, flavonoids, polyphenols, polysaccharides, stilbene and benzofuran. Antioxidant, antitumor and other pharmacological effects. [0003] Plant polysaccharides are natural macromolecular compounds composed of a class of monosacchar...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 何宁佳陈虎余婉莎向仲怀
Owner SOUTHWEST UNIVERSITY
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