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Protein delivery system of specific targeting macrophages

A technology of targeted delivery and macrophages, which can be applied to peptide/protein components, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc. It can solve the problems of rapid release of proteins and other problems.

Inactive Publication Date: 2016-10-05
NANKAI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the disadvantage of this direct absorption preparation method is that the absorbed protein is released from the carrier very quickly.

Method used

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Examples

Experimental program
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Embodiment Construction

[0006] 1. Preparation of β-glucan Particles

[0007] Yeast S.cerevisiae undergoes a series of alkaline and acidic treatment steps to obtain β-glucan particles. Dissolve 3.75g of yeast in 50mL of pure water, adjust the pH value to about 12.0-12.5 with 1.0M NaOH, then heat and stir at 60°C, centrifuge at 2000g for 10min after 1 hour to collect the yeast shell precipitate, and dissolve the precipitate in 50mL of pure water , use 1.0M HCl to adjust the pH value to about 4-5, heat and stir at 55°C for 1 hour, centrifuge to collect the yeast shell precipitate, wash the yeast shell 3 times with water, wash the yeast shell 4 times with isopropanol, acetone Wash 2 times, obtain 1.72g yeast shell powder after vacuum drying.

[0008] 2. Fluorescein and Rhodamine Labeling of β-Glucan Particles and BSA

[0009] 500mg GMP dissolved in 50mL 0.1M Na 2 CO 3 (pH 9.2), 5 mg of FITC or Rhodamine B was dissolved in 2 mL of DMSO, then added dropwise to GMP, and stirred overnight at room tempera...

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PUM

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Abstract

The invention discloses a yeast hollow beta-glucan shell wrapped GMP-BSA protein targeting macrophage delivery system. The system allows BSA to be coated in a yeast shell through using a compact layer formed through the electrostatic adsorption effect of chitosan (CS), tripolyphosphate (TPP), sodium alginate and BSA. GMP-BSA particles prepared in the invention have very good protein release behavior in in-vitro experiments, and can avoid protein loss. The particles highly specifically target the macrophages, such as Raw 264.7 cells, primary BMDM cells (primary bone marrow macrophages) and peritoneal macrophages (PEMs), and are not devoured by NIH3T3, AD293, HeLa, Caco-2 and other non-macrophage tumor cells, and neutral granulocytes in blood. The macrophages play a great role in the pathogenesis of various diseases, and are very important potential target spots. The macrophages are key antigen transfer cells, and are very important in vaccine design. The system can highly selectively deliver various proteins to the macrophages in a targeting manner, and also has very wide application prospect in the field of targeting drug administration and the field of macrophage vaccine design.

Description

Background technique: [0001] Macrophages are white blood cells of the innate immune system that are widely distributed in the body. Macrophages perform various immune functions, activation and immune responses in innate immunity. Therefore, macrophages play an important role in the pathophysiology of various diseases such as macrophage activation syndrome, rheumatoid arthritis, arteriosclerosis, enteritis, and tumorigenesis. Based on the regulatory role of macrophages in diseases, they may be important potential drug targets. This study aims to develop new drugs targeting macrophages to inhibit and treat related diseases. [0002] In addition, macrophages are important antigen-presenting cells, which may effectively induce specific CD8+ cytotoxic T cells to initiate an immune response, and play an early role in the specific defense mechanism of adaptive immunity (acquired immunity) through antigen processing and presentation . Targeted delivery of protein and peptide drugs...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K47/04A61K38/38A61K9/50A61K39/39
Inventor 洪章勇于敏陈泽铭郭文君王晋冯玉鹏孔秀琪
Owner NANKAI UNIV
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