A method of using gibberellin to induce the emergence of dormant buds of Panax notoginseng in vitro

Abstract

The invention discloses a method for inducing seedling emergence of in-vitro panax notoginseng dormant buds through gibberellin. The method includes the steps that field panax notoginseng is taken, dormant buds are cut off and washed, cracked tissue on the surfaces is removed, the obtained dormant buds are soaked with ethyl alcohol of 70% for 1 min to 2 min, washed with sterile water, then soaked with a sodium hypochlorite solution of 10% for 8 min to 10 min and washed with sterile water, the processed dormant buds are planted on MS culturing media with gibberellin and subjected to illumination culturing, seedlings emerge in 6 days to 7 days and transferred into MS culturing media with cytokinin 6-BA and auxin IAA, and the small grow panax notoginseng seedlings are subjected to differentiation rooting in 3 weeks and transplanted to fields for planting. According to the method, the panax notoginseng seedlings cultured in an in-vitro reproduction mode are strong in plant and not prone to infection by pest and disease damage, the problems that panax notoginseng seeds are low in seedling germination rate and difficult to store are solved, seasonal dependence of panax notoginseng planting is overcome, year-around production of panax notoginseng is promoted, and good economic benefits and good application and popularization value are achieved.

Description

technical field [0001] The invention relates to the technical field of Panax notoginseng planting, in particular to a method for inducing the emergence of dormant buds of Panax notoginseng in vitro by using gibberellin. Background technique [0002] Panax notoginseng belongs to the perennial herbaceous plant of Araliaceae. Its stems, leaves and flowers can be used as medicine. It is warm in nature and bitter in taste. The flavonoids in the compound can improve myocardial blood supply, increase blood vessel elasticity, and dilate coronary arteries. Panax notoginseng can lower blood lipids, and can prevent and treat coronary heart disease and angina pectoris. Therefore, the demand for panax notoginseng in the medical field is increasing. The market supply of Panax notoginseng is getting slower and slower. This is mainly due to the long growth cycle of Panax notoginseng, and most of them use seeds to raise seedlings. The cultivation management technology in the prior art is rel...

AI Technical Summary

Problems solved by technology

[0002] Panax notoginseng belongs to the perennial herbaceous plant of Araliaceae. Its stems, leaves and flowers can be used as medicine. It is warm in nature and bitter in taste. The flavonoids in the compound can improve myocardial blood supply, increase blood vessel elasticity, and dilate coronary arteries. Panax notoginseng can lower blood lipids, and can prevent and treat coronary heart disease and angina pectoris. Therefore, the demand for panax notoginseng in the medical field is increasing. The market supply of Panax notoginseng is getting slower and slower. This is mainly due to the long growth cycle of Panax notoginseng, and most of them use seeds to raise seedlings. The cultivation management technology in the prior art is relatively poor. On the one hand, it often causes serious economic losses due to improper storage of seeds. Loss, on the other hand, the germination rate and survival rate of Panax notoginseng seeds are also low

[0003] In recent years, some people have tried to use the dormant buds of Panax notoginseng to grow seedlings. However, since Panax notoginseng is a reduced-rooted plant, the root tubers have obvious dormancy. It takes about 90 days for the dormancy time of the Panax notoginseng seedlings to grow from dormant buds to full germination. And it needs a certain low temperature (<10°C) to break through, so there are fewer relevant reports on the application of dormant buds in actual production.

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