A simple method for rapid detection of verticillium infection in apple branches
A technology for rot fungus and apple branches, which is applied in horticultural methods, botanical equipment and methods, biochemical equipment and methods, etc., and can solve the problem of difficulty in understanding the exact amount of bacteria carried by the tree, researching the control effect of difficult pathogenic bacteria, and restricting the rotation of the plant. Epidemiology of striae disease and research and development of prevention and control technology to achieve the effect of reliable detection results and simple operation methods
Inactive Publication Date: 2019-01-29
李保华 +1
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[0006] Due to the latent infection of apple ring disease, it is difficult for researchers to understand the infection amount of pathogenic bacteria and the control effect of pesticides through inoculation tests, and it is also difficult to know the exact amount of bacteria on the tree, which restricts the development of ring ring disease. Research and development of epidemiology and prevention and control technology
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application example 2
[0022] Application case 2: The detection rate of pathogenic bacteria after inoculation at different times: at the end of July 2014, use 10 per ml 5 The conidia suspension of a ringworm fungus was inoculated into branches of Fuji apples with a diameter of 0.3-1.0 cm, and covered with plastic bags to keep them moist for 24 hours after inoculation; the inoculated branches were marked and divided into 4 groups on average, and 7 days after inoculation respectively. After 1, 14, 21 and 28 days, girdling was performed and treated with paraquat, 24 shoots were treated at each time. From the 5th day after paraquat treatment, the number of shoots with necrotic cortex and the number of shoots with pycnidia of R. verticillium were checked and recorded.
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Abstract
The invention discloses a simple method for rapidly detecting physalospora piricola infection amount in apple branches, and relates to a rapid detection technology for pathogen infection amount in plants. The method is suitable to detect pathogen quantity of living apple branches whose diameter at the base is smaller than 3 cm. The specific method comprises: girdling a cortex on the base of a to-be-detected branch, width being 2-10 mm; using a paraquat liquor whose active ingredient content is 60 g / L - 100 g / L to soak absorbent paper, degreasing cotton, and cotton threads or cloth strips, after taking the absorbent paper, the degreasing cotton, and the cotton threads or cloth strips out and draining, winding the absorbent paper, the degreasing cotton, and the cotton threads or cloth strips on a girdling opening position, using an adhesive tape or a plastic film to wrap tightly; from the third day after processing, inspecting and recording the number of lenticels which are diseased and not diseased on the branches, and whether a necrosis cortex generates pycnidium or not. The method is advantaged in that the method detects clearly whether apple branches have potential physalospora piricola in 1-2 weeks, and the quantity of potential pathogen, and the method is simple in operation method and reliable in detection result, and solves a problem that a conventional survey cannot detect physalospora piricola infection amount in branches.
Description
technical field [0001] The invention belongs to the field of plant disease detection, and relates to a rapid detection technology for pathogen infection in plants. Background technique [0002] Apple ring spot is an important disease on apples, which mainly damages branches and fruits. It is an important disease on apples in China, especially in the production areas around the Bohai Bay and the old course of the Yellow River. In 2008, a survey on the incidence of branch ring disease in 88 orchards in 7 major apple-producing provinces including Shandong showed that the overall incidence rate of apple branch ring disease reached 77.6%. [0003] Ascomycota botrytis Botryosphaeria dothidea Caused by infection, the bacteria mainly infect branches from lenticels, stomata, wounds and other parts, forming tumors, saddle-shaped lesions and dry rot lesions. [0004] Existing studies have found that after the ringworm bacteria infect branches, only a few infecting bacteria induce tum...
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IPC IPC(8): C12Q1/06A01G7/06
Inventor 李保华王玉然董向丽
Owner 李保华