Method for inducing production of Rosa sterilis tetraploid through soaking method

Abstract

The invention discloses a method for inducing production of Rosa sterilis tetraploid through soaking method. The method comprises a first step of preparation of material: removing leaves and tops of aseptic seedling stems; cutting the aseptic seedling stems into 0.8 to 3 cm in length; remaining at least one axillae for each section; a second step of pre-cultivation: pre-cultivating the stems in a culture medium MS+ 0.5mg / L 6-BA for 2 to 3 days; a third step of vibration in a manner of avoiding light: soaking the pre-treated material into a sterile water containing 200 to 500mg / L of colchicine; vibrating the material on a shaking table at 120r / min and 25 to 30 DEG C for 12 to 72 hours in a manner of avoiding light; a fourth step of light cultivation: after being soaked, transferring the material into a culture medium MS+ 0.15mg / L NAA+ 0.5mg / L 6-BA without containing colchicine to be cultivated continuously; a fifth step of homogenization treatment for variant buds: after the cultivation in the fourth step is performed for 40 days, selecting the buds which are obviously variant, repeatedly cultivating the buds in the culture medium MS+ 0.15mg / L NAA+ 0.5mg / L 6-BA five times at interval of 40 days. By the inducing method disclosed by the invention, the new germplasm of the tetraploid Rosa sterilis is obtained, so that the nutrient contents like Vitamin C, Vitamin E, SOD (superoxidase dismutase) and the like of Rosa sterilis fruits can be increased, and meanwhile the fruit width and edible part are increased.

Description

technical field [0001] The invention relates to the technical field of an induction method for producing tetraploids of roxburghii by soaking, in particular to a method for inducing tetraploids of roxburghii by soaking. Background technique [0002] Seedless prickly pear (Rosasterilis S.D.Shi) is a perennial climbing shrub of the genus Rosa in the family Rosaceae. It is one of the endemic species in China, and it is a relative species of prickly pear (R.roxbunghii). Its wild resources are mainly distributed in the north of Anshun City, Guizhou Province and the south of Guiyang City. In recent years, a large number of species have been introduced and planted in many places. Seedless Rosa roxburghii has high medicinal health care, sightseeing, ecological protection and economic value. Compared with ordinary thorn pears, the seedless thorn pears are seedless or have 1-2 seeds, less tannin content, higher sugar content, thick flesh and sweet fruit flavor, no obvious astringenc...

AI Technical Summary

Problems solved by technology

For the improvement of seedless Rosa roxburghii varieties, only Wei Jingfeng et al. have carried out studies on single plant selection, but there has been no report on ploidy breeding

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