Method for removing free gossypol in cottonseed meal through step-by-step reduction

A technology of free gossypol and cottonseed meal, which is applied in the field of step-by-step reduction of free gossypol in cottonseed meal, can solve the problems of failure to achieve the ideal detoxification rate, waste water discharge pollutes the environment, and low removal efficiency. Broad market application prospects, high dephenolization efficiency and low energy consumption

Inactive Publication Date: 2016-10-26
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Common types of microbial fermentation include wet fermentation and solid-state fermentation. Wet fermentation soaks the substrate and bacteria in the liquid, so the fermentation effect is good, and the removal rate of free gossypol can reach more than 90

Method used

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  • Method for removing free gossypol in cottonseed meal through step-by-step reduction
  • Method for removing free gossypol in cottonseed meal through step-by-step reduction
  • Method for removing free gossypol in cottonseed meal through step-by-step reduction

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, step-by-step method reduce free gossypol content in cottonseed meal. The specific method is as follows, and the percentages are mass percentages:

[0022] Cottonseed meal is sieved with 40 mesh, and 80g of cottonseed meal, 12g of bran, and 8g of corn flour are used as substrates. Bacillus subtilis and yeast strains are activated, Bacillus subtilis and yeast are mixed at a ratio of 1:1, the inoculum size is 6%, ammonium sulfate is added 0.5%, the initial moisture content is 50%, the fermentation temperature is 30°C, and the fermentation time is 48h. After fermentation, the wet-based cottonseed meal is processed by microwave technology. Under the conditions of microwave power of 800w, microwave time of 3min, and microwave temperature of 80°C, the second detoxification treatment is carried out, and then dried, crushed, finished product, and packaged .

Embodiment 2

[0023] Embodiment 2, step-by-step method reduce free gossypol content in cottonseed meal. The specific method is as follows, and the percentages are mass percentages:

[0024] Cottonseed meal is sieved with 40 meshes, and 85g of cottonseed meal, 10g of bran, and 5g of corn flour are used as substrates. Bacillus natto and yeast are activated, Bacillus natto and yeast are mixed at a mass ratio of 2:1, the inoculum size is 8%, uric acid is added 1%, the initial moisture content is 60%, the fermentation temperature is 30°C, and the fermentation time is 48h. After fermentation, the wet-based cottonseed meal is processed by microwave technology. Under the conditions of microwave power of 1000w, microwave time of 3min, and microwave temperature of 100°C, the second detoxification treatment is carried out, and then dried, crushed, finished product, and packaged .

Embodiment 3

[0025] Embodiment 3, step-by-step method reduces the content of free gossypol in cottonseed meal. The specific method is as follows, and the percentages are mass percentages:

[0026] Cottonseed meal is sieved with 40 meshes, and 85g of cottonseed meal, 10g of bran, and 5g of corn flour are used as substrates. Bacillus subtilis and Candida utilis are activated, Bacillus subtilis and Candida utilis are mixed at a ratio of 1:2, the inoculum size is 10%, ammonium sulfate is added 1%, the initial moisture content is 60%, the fermentation temperature is 35°C, and the fermentation Time 48h. After fermentation, the wet-based cottonseed meal is processed by microwave technology. Under the conditions of microwave power of 1000w, microwave time of 5min, and microwave temperature of 60°C, the second detoxification treatment is carried out, and then dried, crushed, finished product, and packaged .

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Abstract

The invention discloses a method for removing free gossypol in cottonseed meal through step-by-step reduction and solves the problems of low detoxification rate, high energy and water consumption and the like of conventional cottonseed meal. The technical scheme of the method comprises steps as follows: step one, cottonseed meal is smashed and then is mixed uniformly with bran and corn meal, and a mixture is taken as a substrate for standby; step two, activated bacillus and saccharomycete strains are mixed with the substrate and then subjected to solid-state fermentation, and wet based cottonseed meal is obtained; step three, the wet based cottonseed meal is subjected to microwave inactivation and detoxification and then is dried and smashed, and a finished cottonseed meal product is obtained. The method adopts a simple technology and a short detoxification process, is safe, reliable and environment-friendly and has low water and energy consumption.

Description

technical field [0001] The invention relates to the technical field of feed processing, in particular to a step-by-step method for removing free gossypol in cottonseed meal. Background technique [0002] Cottonseed meal is a by-product of the oil industry. Its protein content is generally 35% to 45%, and the arginine content in protein is as high as 3.6% to 3.8%. It has high nutritional value and can be used as a high-quality Feed raw materials, but due to the harmful component free gossypol in cottonseed meal, the amount of cottonseed meal added to feed is limited. [0003] In order to reduce the free gossypol in cottonseed meal, technicians have carried out extensive research, and the microbiological fermentation method, physical method, chemical method and microwave extraction method found in reports. [0004] Although the chemical method has high extraction efficiency of free gossypol, it is unfavorable to animal health when used in animal feed due to the introduction o...

Claims

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Application Information

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IPC IPC(8): A23L5/20
Inventor 王春维程熠娜祝爱侠陶冶程铭刘世操陈帆卜小丽
Owner WUHAN POLYTECHNIC UNIVERSITY
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