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Application of bacillus coagulans to preparation of medicine or food for preventing and/or treating anaphylactic reaction

A technology for Bacillus coagulans and allergic reaction, which is applied in the field of functional food composition and preparation thereof, and can solve the problems of easily causing allergic reaction and the like

Active Publication Date: 2016-10-26
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Penaeus vannamei is very popular among consumers due to its delicious taste and rich in nutritious protein, but its muscle protein contains a large amount of tropomyosin, which can easily cause allergic reactions

Method used

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  • Application of bacillus coagulans to preparation of medicine or food for preventing and/or treating anaphylactic reaction
  • Application of bacillus coagulans to preparation of medicine or food for preventing and/or treating anaphylactic reaction
  • Application of bacillus coagulans to preparation of medicine or food for preventing and/or treating anaphylactic reaction

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] In the present invention, the specific IgE and IgG levels in serum, histamine content, cytokine content in cell supernatant, cell RNA expression and other indicators are used to evaluate the regulatory effect of bacillus coagulans on allergic reaction.

[0033] 1.1 Establishment of sensitized animal model

[0034] Experimental animals: detergent female Balb / c mice, 6 weeks old, weighing 18±2g, purchased from the Experimental Animal Center of Hangzhou Normal University, fed for two consecutive generations with a special feed that does not contain shrimp protein. The temperature of the rat room is 20±2°C, the humidity is 50±10%, and the day and night cycle is 12 hours to ensure ventilation. The rat cage is cleaned and disinfected twice a week. Using the method of random grouping, set up the blank control group, the allergy group, and the bacteria experiment group respectively, with 8 mice in each group.

[0035]Strain culture: Bacillus coagulans with the preservation num...

Embodiment 2

[0055] This example illustrates the effect of Bacillus coagulans of CCTCC NO: M 2013193 on Treg cells, mast cells and B cells.

[0056] 2.1 Sources of Treg cells, B cells and mast cells

[0057] Take 6-week-old female Balb / c mice with a body weight of 18-22 g. After cervical sacrifice, the spleen was aseptically removed and ground to obtain spleen lymphocytes. The above two types of cells were sorted by flow cytometry.

[0058] P815 mast cells were purchased from Shanghai Gefan Co., Ltd., and the culture conditions were: 37°C, 5% CO 2 .

[0059] 2.2 Co-culture of Bacillus coagulans with Treg cells and Th2 cells

[0060] Adjust the concentrations of Treg cells and Th2 cells to 10 6 individual / mL. In a 6-well plate, add 1 mL each of Treg cells and Th2 cells, 100 μL of Bacillus coagulans (concentration of 10 8 CFU / mL), tropomyosin was stimulated in vitro after filter sterilization (stimulating concentration was 50 μg / mL). 37°C, 5% CO 2 Conditioned for 48h. Centrifuge at...

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Abstract

The invention discloses application of bacillus coagulans to the preparation of medicine or food for preventing and / or treating anaphylactic reaction. The collection number of the bacillus coagulans is CCTCC NO: M 2013193. Compared with other bacillus coagulans in the same category, the bacillus coagulans with the collection number of CCTCC NO: M 2013193 can be used for regulating the body immunity more effectively. Experiment results show that a living bacterium agent prepared from the bacillus coagulans with the collection number of CCTCC NO: M 2013193 can be used for effectively relieving the aquatic product anaphylactic reaction caused by tropomyosin, can effectively promote the polarization of Th0 cells in a direction towards Th1 and Treg, and can reduce the IgE antibody level and mast cell degranulation.

Description

technical field [0001] The present invention relates to the application of Bacillus coagulans, specifically, the application of Bacillus coagulans CCTCC NO: M 2013193 with anti-allergic effect in regulating the immune function of the body. The present invention also relates to a functional food composition and its preparation method. Background technique [0002] IgE-mediated immediate food allergic reactions mainly include two stages: the sensitization activation phase and the effect phase of food allergens. Naive allergen exposure stimulates intestinal mucosal antigen-presenting cell activation and potent activation of naive CD4 + T cells proliferate and differentiate into helper T cell (Th cells) subsets, among which Th2 cell subsets mainly mediate humoral immune responses related to allergic reactions and parasitic infections, and secrete cells such as IL-4, IL-5 and IL-13 Factors that induce B cell class switching and produce allergen-specific IgE, which can bind to t...

Claims

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Application Information

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IPC IPC(8): A61K35/742A61P37/02A61P37/08A23L33/135
CPCA23V2002/00A61K35/742A23V2200/304
Inventor 傅玲琳王彦波赵淑淑
Owner ZHEJIANG GONGSHANG UNIVERSITY
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