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Method using ice crystal template method to prepare three-dimensional biological scaffold with regular lamellar structure and application of method

A layered structure, three-dimensional biological technology, applied in the fields of application, non-active components of polymer compounds, medical science, etc., can solve problems hindering the application of three-dimensional porous scaffolds, achieve improved cell compatibility and mechanical properties, and biological safety High, improve the effect of mechanical properties

Active Publication Date: 2016-10-26
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above pore-making methods have seriously hindered the application of three-dimensional porous scaffolds in biomedicine

Method used

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  • Method using ice crystal template method to prepare three-dimensional biological scaffold with regular lamellar structure and application of method
  • Method using ice crystal template method to prepare three-dimensional biological scaffold with regular lamellar structure and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] (1) The silk fibroin fibers obtained after degumming the silkworm cocoons are successively dissolved, filtered, dialyzed and centrifuged, and then concentrated to a silk fibroin aqueous solution with a mass percentage of 2%;

[0036] (2) Prepare the ice crystal / silk fibroin scaffold by the ice crystal template method, inject the silk fibroin aqueous solution into the mold, place the mold on the gradient cooling device, adjust the cooling rate at a constant speed to 0.1°C / min, and after 240 minutes, the inside of the mold The scaffold was lowered to -24°C to obtain a frozen ice crystal / silk fibroin scaffold;

[0037] (3) The regular lamella ice crystals in the ice crystal / silk fibroin scaffold were removed by freeze-drying to obtain a silk fibroin scaffold with a regular lamellar structure, while the morphology of the scaffold in the control group (prepared by freeze-drying method) was irregular and porous shape;

[0038] (4) The scanning electron microscope of the silk...

Embodiment 2

[0041] (1) The silk fibroin fibers obtained after degumming the silkworm cocoons are successively dissolved, filtered, dialyzed and centrifuged, and then concentrated to a silk fibroin aqueous solution with a mass percentage of 25%;

[0042] (2) The ice crystal / silk fibroin scaffold is prepared by the ice crystal template method. The silk fibroin aqueous solution is injected into the mould, the mold is placed on the gradient cooling device, and the cooling rate is adjusted to 100°C / min at a constant speed. The scaffold was lowered to -100°C in a constant cooling method to obtain a frozen ice crystal / silk fibroin scaffold;

[0043] (3) remove the ice crystals / regular lamellar ice crystals in the silk fibroin scaffold by freeze-drying to obtain a silk fibroin scaffold with a regular lamellar structure, and make the silk fibroin scaffold structure of the lamellar structure beta after 75% alcohol treatment;

[0044] (5) Cell experiments showed that the scaffold could induce human ...

Embodiment 3

[0046] (1) The silk fibroin fibers obtained after degumming the silkworm cocoons are successively dissolved, filtered, dialyzed and centrifuged, and then concentrated to a silk fibroin aqueous solution with a mass percentage of 12%;

[0047] (2) Prepare the ice crystal / silk fibroin scaffold by the ice crystal template method, inject the silk fibroin protein aqueous solution into the mold, place the mold on the gradient cooling device, adjust the cooling rate to 5°C / min at a constant speed, and after 20 minutes, the mold inside The scaffold was lowered to -100°C in a constant cooling method to obtain a frozen ice crystal / silk fibroin scaffold;

[0048] (3) remove the ice crystals / regular lamellar ice crystals in the silk fibroin scaffold by freeze-drying to obtain a silk fibroin scaffold with a regular lamellar structure, and make the silk fibroin scaffold structure of the lamellar structure beta after 100% methanol treatment;

[0049] (5) Cell experiments showed that the regul...

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Abstract

The invention discloses a method using an ice crystal template method to prepare a three-dimensional biological scaffold with a regular lamellar structure and application of the method. The method includes: sequentially subjecting fibroin fibers obtained after degumming silkworm cocoons to dissolving, filtering, dialysis and centrifuging, concentrating to obtain a silk fibroin aqueous solution, using the ice crystal template method to prepare an ice crystal / fibroin scaffold, using a constant cooling speed to perform freezing treatment, and using freeze drying to remove the regular lamellar ice crystals in the ice crystal / fibroin scaffold so as to obtain a fibroin scaffold, serving as the three-dimensional biological scaffold with the regular lamellar structure, with the regular lamellar structure. By the arrangement, the method has the advantages that the scaffold prepared by the method is excellent in biocompatibility and mechanical performance and evidently increases the proliferation performance of human cells and the directional differentiation performance of stem cells, and accordingly the bone tissue repairing effect of the biological scaffold is increased; the method is promising in application prospect in fields such as tissue engineering, drug sustained release, hemostatic materials and filtering membranes.

Description

technical field [0001] The invention discloses a method and application for preparing a three-dimensional biological scaffold with a regular lamellar structure by an ice crystal template method and belongs to the field of biomedical materials. Background technique [0002] Silk protein biomaterials represented by silkworm silk fibroin, tussah silk fibroin and spider silk protein have excellent mechanical properties, biocompatibility, no immune rejection, degradability, and are essentially the structural characteristics of natural proteins. The field of materials has received high attention. Silk protein biomaterials can be used as biomaterials with different shapes and functions, such as bone tissue repair, wound covering materials, drug sustained release materials, etc., to meet the different needs of human beings for biomaterials. [0003] Porous materials can be used to regenerate wounded tissue in the field of tissue engineering. Therefore, in addition to biocompatibili...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/50A61L27/56A61L27/58A61L24/10A61L24/00A61K47/42
Inventor 杨明英帅亚俊毛传斌
Owner ZHEJIANG UNIV