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A kind of method that utilizes grape seed extract to prepare oligomeric proanthocyanidins

A technology for oligomeric proanthocyanidins and proanthocyanidins is applied in the field of separation and preparation of proanthocyanidins, which can solve the problems of difficulty in separately extracting oligomer and high polymer procyanidins, limited separation precision of macroporous adsorption resin, and low yield of oligomer procyanidins, and the like. The effect of clear purpose, stable color and remarkable performance

Active Publication Date: 2018-04-24
HUNAN XINLI BIOLOGICAL SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantage of this method is: proanthocyanidins are soluble in water and ethanol, and are not affected by pH, so it is difficult to extract monomers, oligomers and high polymers of proanthocyanidins in the pH range of 4.0 to 4.4; in addition, macroporous The separation accuracy of the adsorption resin is limited, and the monomer, oligomer and high polymer proanthocyanidin cannot be completely separated, and it is difficult to obtain the oligomer product with a polymerization degree of only 2-4
The main disadvantage of this method is that the high-polymer proanthocyanidins can only be partially separated by cold dissolution; although the high-polymer proanthocyanidins can be completely removed by nanofiltration, the molecular weight cut-off interval distribution of the nanofiltration membrane will reduce the degree of polymerization to 3-5. Oligomer proanthocyanidins interception, low yield of oligomer proanthocyanidins, large waste of raw materials, high production cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (1) Preparation of ethanol solution of proanthocyanidin

[0026] Weigh a certain amount of grape seed extract, according to the ratio of grape seed extract mass / anhydrous ethanol volume ratio of 1:50kg / L, add it to the anhydrous ethanol under stirring, and then suction filter after 10min treatment, and collect them separately Filter residue and filtrate, the collected filter residue mainly contains high polymer proanthocyanidins with a degree of polymerization of more than 10, which are used to prepare feed antioxidants; the collected filtrate is an ethanol solution of proanthocyanidins, which is used in the next step to prepare the initial separation concentrate.

[0027] (2) Preparation of the initial separation concentrate

[0028] After the completion of step (1), pump the ethanol solution of proanthocyanidin collected in step (1) into a vacuum concentrator, and conduct vacuum concentration at a temperature of 60°C and a vacuum degree of 0.06MPa until the volume of the con...

Embodiment 2

[0038] (1) Preparation of ethanol solution of proanthocyanidin

[0039] Weigh a certain amount of grape seed extract, according to the ratio of grape seed extract mass / anhydrous ethanol volume ratio of 1:75kg / L, add it to the anhydrous ethanol under agitation, treat for 20 minutes and then suction filter and collect them separately Filter residue and filtrate, the collected filter residue mainly contains high polymer proanthocyanidins with a degree of polymerization of more than 10, which are used to prepare feed antioxidants; the collected filtrate is the ethanol solution of proanthocyanidins and is used in the next step to prepare the initial separation concentrate.

[0040] (2) Preparation of the initial separation concentrate

[0041] After the completion of step (1), pump the proanthocyanidin ethanol solution collected in step (1) into a vacuum concentrator, and conduct vacuum concentration at a temperature of 70°C and a vacuum degree of 0.07 MPa until the volume of the concentr...

Embodiment 3

[0051] (1) Preparation of ethanol solution of proanthocyanidin

[0052] Weigh a certain amount of grape seed extract, according to the ratio of grape seed extract mass / anhydrous ethanol volume ratio of 1:100kg / L, add it to the anhydrous ethanol under stirring, suction filter after 30 minutes of treatment, and collect separately Filter residue and filtrate, the collected filter residue mainly contains high polymer proanthocyanidins with a degree of polymerization of more than 10, which are used to prepare feed antioxidants; the collected filtrate is the ethanol solution of proanthocyanidins and is used in the next step to prepare the initial separation concentrate.

[0053] (2) Preparation of the initial separation concentrate

[0054] After the completion of step (1), pump the ethanol solution of proanthocyanidin collected in step (1) into a vacuum concentrator, and conduct vacuum concentration at a temperature of 80°C and a vacuum of 0.08 MPa until the volume of the concentrated sol...

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Abstract

The invention provides a method for preparing oligomeric proanthocyanidins by utilizing a grape seed extract, belonging to the technical field of separation and preparation of the oligomeric proanthocyanidins. According to the invention, oligomeric proanthocyanidins freeze-dried powder and polymeric proanthocyanidins freeze-dried powder are prepared through dissolving, filtering, vacuum concentration, ultrafiltration, column filling, elution, nanofiltration and freeze-drying by using the grape seed extract as a raw material. The method provided by the invention has high raw material utilization rate, high separation accuracy, good separation effect, high product purity and clear product use, realizes complete utilization of raw materials and complete recycling of the solvent, is free of three-wastes discharge, and is a typical high-tech green process. The oligomeric proanthocyanidins prepared by using the method provided by the invention can be extensively applied in the fields of medicines, health-care products, etc.

Description

1. Technical Field [0001] The invention belongs to the technical field of separation and preparation of proanthocyanidins, and specifically relates to a method for preparing oligomeric proanthocyanidins by using grape seed extracts. 2. Background technology [0002] Oligomeric proanthocyanidins are derived from plants and are composed of proanthocyanidins with a degree of polymerization of less than 5. It has the advantages of anti-oxidation, anti-radiation, and chelating metal ions. It is widely used in the fields of medicine and health care. It is a kind of high economic value Of biologically active substances. [0003] Existing methods for preparing oligomeric proanthocyanidins, such as the invention patent entitled "A method for preparing oligomeric proanthocyanidins" with the application number CN201410140039.3, use 60-70% ethanol at pH 4.0-4.4 The monomers, oligomers and high polymer proanthocyanidins are extracted, and then the macroporous resin is used for adsorption and s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/62C12G1/00
CPCC07D311/62C12G1/00
Inventor 徐扬陶仁友江文杨洋石业新袁德宽余红梅周小华
Owner HUNAN XINLI BIOLOGICAL SCI & TECH
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