A medium bottle for preparing blood agar plates

Abstract

The invention discloses a culture medium bottle for preparing blood agar plates. The culture medium bottle includes a bottle body. Scale lines are formed on the external wall of the bottle body. A sample adding neck and a pour-out neck are arranged on the upper end of the bottle body and are respectively inclined towards the left and the right sides. A built-in plug core and a sealing cover are arranged in the pour-out neck. The plug core is seamlessly inserted in the pour-out neck in a manner of sealing the inner wall. A double-layer convex arc-shaped filter screen is arranged on the lower end of the plug core. The upper end of the plug core is screwed tightly with the sealing cover through threads. An air permeable cover, wherein a plurality of air permeable pores are uniformly distributed in the surface of the cover, is arranged on the sample adding neck. The air permeable cover is fixedly screwed with the sample adding neck through threads. By means of the plug core having the double-layer filter screen, large quantity of foam, which is generated due to quick shaking, on the surface of a liquid fresh blood culture medium in the bottle body can be effectively removed, so that the poured blood agar culture medium, after solidification, can form a smooth, flat and uniform surface. The culture medium bottle ensures quality of the blood agar culture medium and also improves operation efficiency, has a reasonable structural design, is convenient to use in experiment, is simple in experiment parameter control, and is available to promote.

Description

technical field [0001] The invention belongs to the technical field of microbial experiment equipment, and relates to a culture medium bottle, in particular to a culture medium bottle for preparing blood agar plates. Background technique [0002] Blood agar medium is a beef extract peptone medium containing defibrated animal blood (generally rabbit blood or sheep blood). Heme (factor X) and other special growth factors are often used in microbiological tests to cultivate, separate and preserve certain pathogenic microorganisms that are demanding on nutrients; in addition, they can also be used to determine the hemolysis of bacteria. In view of the harsh requirements of some bacteria on the preparation of blood agar medium, for example, after the medium is sterilized, it needs to be kept warm at about 45°C, and sterile defibrinated sheep blood is added in proportion, mixed thoroughly, and then poured into a sterilized petri dish. If the temperature is too high, the color of ...

AI Technical Summary

Problems solved by technology

However, the following problems often exist during the test operation: (1) In the process of fully mixing the medium and defibrinated sheep blood, a large amount of foam is often produced floating on the liquid surface of the mixed medium. Or heat to remove the foam, but with the extension of the operation time and the drop of the medium temperature, the medium components are prone to uneven distribution, or even solidification, and a large amount of foam will be produced when mixed again, and the foam removal rate is low. ; When pouring into a sterile plate, the foam floating on the liquid surface is poured into the plate together with the medium. After the medium solidifies, many pits of different sizes are often formed on the surface (caused by foam bursting), which cannot be prepared. The smooth and flat surface of the culture medium required by the test requirements often scratches the surface of the agar during the subsequent streaking and coating operations, which affects the accuracy of the test results

Since the agar medium needs to be kept at a temperature of about 50°C when the agar medium is poured, the amount of the poured plate must be fast and accurate during the pouring process. If the pouring time is too long, the agar and the Defiberized sheep blood will produce uneven distribution, resulting in uneven nutritional components between agar plates, which cannot meet the uniformity of experimental conditions and widen the difference in experimental results

(3) At present, Erlenmeyer flasks with silica gel plugs and tin foil seals are used to sterilize the culture medium. After disinfection, there are problems such as easy air leakage and rupture of the seals.

The use of ordinary plugged reagent bottles, due to the non-ventilated design of the bottle mouth, needs to loosen the bottle mouth for sterilization during the autoclaving process, which may easily cause medium overflow and pollute the built-in warehouse and water layer of the high-pressure steam sterilizer and other hidden dangers

In addition, the mouth size, inclination angle and bottle cap design of ordinary corked reagent bottles do not meet the ergonomic design requirements, and there is a problem of inconvenient use during operation.

(4) The body of the traditional glass Erlenmeyer flask is not easy to grasp. In addition, after the Erlenmeyer flask is autoclaved, the temperature of the bottle body is too hot when it is taken out of the pressure cooker, which increases the difficulty of grasping, easily burns hands, and is difficult to operate , increase the time for pouring and dispensing blood plates; while the temperature of the bottle body must be controlled within the range of 50~55°C when pouring, due to the slow operation process, the unfinished culture medium in the Erlenmeyer flask is often close to solidification and needs to be reheated , Pour the blood plate again after melting, thus increasing the probability of medium contamination, resulting in inconsistent temperature of the poured plate medium, which is likely to cause a large difference in the repeated parallel test results of the medium

(5) If the amount of culture medium prepared in the bottle is too much, it is difficult to control the amount poured into each petri dish when pouring the plate at the beginning. When the culture medium is poured, the bottle mouth is prone to backflow, which is easy to cause pollution, and the operation is time-consuming and laborious. Preparation It is difficult for the blood plate to meet the unified standard requirements, and it cannot meet the requirements in the comparative culture experiment. At present, there is no good solution for this

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