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A kind of Cordyceps sinensis liquid culture medium and preparation technology thereof

A liquid culture medium, Cordyceps sinensis technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., to achieve the effect of rich active ingredients and excellent quality

Active Publication Date: 2019-08-27
青海珠峰冬虫夏草工程技术研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In the prior art, there is no report of using Jerusalem artichoke for the fermentation medium of Cordyceps sinensis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation of embodiment 1 Jerusalem artichoke alcohol extract

[0022] Pulverize Jerusalem artichoke tubers or stems and leaves, add 70% ethanol by volume according to the ratio of material to liquid 1:15, heat and reflux for extraction, remove ethanol, concentrate, and dry to obtain the product.

Embodiment 2

[0023] The preparation of embodiment 2 Jerusalem artichoke water extract

[0024] Crush Jerusalem artichoke tubers or stems and leaves, add distilled water at a ratio of 1:12, extract at 85°C for 2 hours, repeat three times, filter, combine the filtrates, concentrate under reduced pressure, precipitate with 7 times the volume of 90% ethanol for 3 times, filter, and The precipitate is dried and obtained.

Embodiment 3

[0026] (1) Take the raw material by weight percentage as follows: 5% of Jerusalem artichoke alcohol extract of embodiment 1, 5% of oat flour, 6% of sorghum husk powder, 6% of peptone, 0.05% of lysine, 0.05% of glycine, KH 2 PO 4 0.1%, Fe 2 SO 4 0.04%, water balance;

[0027] (2) adding oat flour and sorghum husk powder into water, heating and boiling;

[0028] (3) the Jerusalem artichoke alcohol extract of embodiment 1, peptone, lysine, glycine, KH 2 PO 4 , Fe 2 SO 4 Add in the solution of step (1), stir evenly;

[0029] (4) The mixture obtained in step (3) was sterilized at 121° C. for 30 minutes at high temperature, and then cooled to room temperature to obtain the culture medium.

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PUM

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Abstract

The invention relates to a liquid culture medium for cordyceps and a process for preparing the liquid culture medium. The liquid culture medium for the cordyceps comprises, by weight, 5-15% of Jerusalem artichoke extract, 1-5% of oat powder, 3-6% of sorghum husk powder, 2-6% of peptone, 0.01-0.05% of lysine, 0.01-0.05% of glycine, 0.05-0.1% of KH2PO4, 0.02-0.05% of Fe2SO4 and the balance water. The liquid culture medium and the process have the advantages that the Jerusalem artichoke extract is used for preparing the liquid fermentation culture medium for the cordyceps, accordingly, cordyceps fermented products prepared by the aid of the liquid fermentation culture medium are excellent in quality and contain active components with high contents, and the contents of cordycepin, adenosine, cordyceps polysaccharides and cordyceps acid are equivalent to the contents of cordycepin, adenosine, cordyceps polysaccharides and cordyceps acid in natural cordyceps.

Description

technical field [0001] The invention relates to the technical field of edible and medicinal fungus fermentation production, in particular to a liquid culture medium of Cordyceps sinensis and a preparation process thereof. Background technique [0002] Cordyceps sinensis, also known as Cordyceps militaris and Cordyceps militaris, is formed by connecting the insect body and the sub-seat, with a total length of 9 to 12 cm, a dark brown color, and many horizontal wrinkles on the back. The insect body is light and brittle, easy to break, and the cross-section is white. Slightly elastic; sub-seat grows from the head of the worm, 4-7cm in length, the base covers the head of the worm, in the shape of a long rod, thick at the top and thin at the bottom, taupe or dark brown, flexible and flexible, with many cross-sections is hollow. Smell with straw mushroom aroma, sweet. Semen solitary, slender cylindrical, 4 to 7 cm long, about 3 mm in diameter; surface dark brown to tan, with fin...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12R1/645
Inventor 王玉花王辉王茜贾得儒董笑菲王晓燕杨成香
Owner 青海珠峰冬虫夏草工程技术研究有限公司
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