A kind of Cordyceps sinensis liquid culture medium and preparation technology thereof
A liquid culture medium, Cordyceps sinensis technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, fungi, etc., to achieve the effect of rich active ingredients and excellent quality
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Embodiment 1
[0021] The preparation of embodiment 1 Jerusalem artichoke alcohol extract
[0022] Pulverize Jerusalem artichoke tubers or stems and leaves, add 70% ethanol by volume according to the ratio of material to liquid 1:15, heat and reflux for extraction, remove ethanol, concentrate, and dry to obtain the product.
Embodiment 2
[0023] The preparation of embodiment 2 Jerusalem artichoke water extract
[0024] Crush Jerusalem artichoke tubers or stems and leaves, add distilled water at a ratio of 1:12, extract at 85°C for 2 hours, repeat three times, filter, combine the filtrates, concentrate under reduced pressure, precipitate with 7 times the volume of 90% ethanol for 3 times, filter, and The precipitate is dried and obtained.
Embodiment 3
[0026] (1) Take the raw material by weight percentage as follows: 5% of Jerusalem artichoke alcohol extract of embodiment 1, 5% of oat flour, 6% of sorghum husk powder, 6% of peptone, 0.05% of lysine, 0.05% of glycine, KH 2 PO 4 0.1%, Fe 2 SO 4 0.04%, water balance;
[0027] (2) adding oat flour and sorghum husk powder into water, heating and boiling;
[0028] (3) the Jerusalem artichoke alcohol extract of embodiment 1, peptone, lysine, glycine, KH 2 PO 4 , Fe 2 SO 4 Add in the solution of step (1), stir evenly;
[0029] (4) The mixture obtained in step (3) was sterilized at 121° C. for 30 minutes at high temperature, and then cooled to room temperature to obtain the culture medium.
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