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Angiotensin-converting enzyme inhibitory peptide and its preparation method and application

An angiotensin and inhibitory peptide technology, applied in the field of biomedicine, can solve problems such as elevated blood pressure, and achieve the effects of strong operability, good application prospects, and reasonable process design

Inactive Publication Date: 2019-03-29
南京可馨生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Angiotensin Converting Enzyme (ACE) is a zinc ion-dependent dipeptide carboxypeptidase that is widely present in mammalian tissues and can convert angiotensin Ⅰ (Angiotensin Ⅰ) into angiotensin Ⅱ ( Angiotensin Ⅱ), the latter is a strong vasoconstrictor, which promotes the increase of blood pressure; on the other hand, ACE can convert bradykinin (bradykinin), which has vasodilation effect, into an inactive fragment, inhibiting the antihypertensive system-activation Peptidrelase-Kinin System (Kallikrein-Kinin System, KKS), which also causes blood pressure to increase

Method used

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  • Angiotensin-converting enzyme inhibitory peptide and its preparation method and application
  • Angiotensin-converting enzyme inhibitory peptide and its preparation method and application
  • Angiotensin-converting enzyme inhibitory peptide and its preparation method and application

Examples

Experimental program
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Effect test

Embodiment 1

[0039] A method for preparing an angiotensin-converting enzyme inhibitory peptide, comprising the following steps:

[0040] (1) Preparation of variegated clam enzymatic hydrolyzate:

[0041] Wash the variegated clam soft and silt, add 3 times the amount of water to decoct twice, 40 minutes each time, separate the decoction and meat dregs, drain; take the meat dregs, add 3 times the amount of water to homogenize, add enzyme activity 30000U / g of trypsin enzymolysis, the weight of trypsin added is 0.60% of the weight of meat dregs, the temperature of enzymolysis is 45°C, the pH of enzymolysis is 8.50, and the reaction time of enzymolysis is 2h; after the end of enzymolysis, Inactivate in a boiling water bath, then add a certain amount of absolute ethanol for alcohol precipitation, and finally form a 70% alcohol solution, and let it stand at 4°C for 24 hours, then take the supernatant, concentrate, and freeze-dry to obtain freeze-dried pink;

[0042] (2) Purification by ion exch...

Embodiment 2

[0046] Example 2 Sequence Analysis of Angiotensin Converting Enzyme Inhibitor Peptide

[0047] Take the angiotensin-converting enzyme inhibitory peptide prepared in Example 1, and use Nano-LC-ESI-MS / MS to analyze the amino acid sequence of the polypeptide. The mass spectrometry conditions are: ESI source, scanning mode: positive ion mode, mass scanning range: 50 ~1000m / z; The molecular weight of the active polypeptide obtained by analysis is 1477.80Da, and the mass spectrometry detection picture is as follows figure 1 As shown, the determined amino acid sequence is: Asn-Thr-Leu-Thr-Leu-Ile-Asp-Thr-Gly-Ile-Gly-Met-Thr-Lys.

Embodiment 3

[0048] Example 3 Synthesis of angiotensin-converting enzyme inhibitory peptide

[0049] According to the amino acid sequence obtained in Example 2, the angiotensin-converting enzyme inhibitory peptide Asn-Thr-Leu-Thr-Leu-Ile-Asp-Thr-Gly-Ile-Gly-Met-Thr- Lys, the purity of the synthesized polypeptide was 98% by HPLC analysis, the molecular weight determined by mass spectrometry was 1477.80Da, which was consistent with the molecular weight of the purified polypeptide, and the fragments of the secondary mass spectrum were consistent with the purified polypeptide fragments.

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Abstract

The present invention discloses an angiotensin converting enzyme inhibiting peptide and a preparation method and application thereof; an amino acid sequence of the inhibiting peptide is Asn-Thr-Leu-Thr-Leu-Ile-Asp-Thr-Gly-Ile-Gly-Met-Thr-Lys. Modern biochemical techniques are utilized to carry out tracking screening and evaluation on active ingredients in Venerupis variegata that inhibit angiotensin converting enzyme, the polypeptide is prepared through purification means such as enzymatic hydrolysis, ethanol precipitation, ion exchange chromatography and reverse phase high-performance liquid chromatography, and experimental results show that the inhibiting polypeptide can well inhibit angiotensin converting enzyme, has good antihypertensive effect, is good in safety and has a promising application prospect.

Description

technical field [0001] The invention relates to an active polypeptide, in particular to a polypeptide with ACE-inhibiting activity isolated from an enzymolyzed clam mollusc, and belongs to the technical field of biomedicine. Background technique [0002] Angiotensin Converting Enzyme (ACE) is a zinc ion-dependent dipeptide carboxypeptidase that is widely present in mammalian tissues and can convert angiotensin Ⅰ (Angiotensin Ⅰ) into angiotensin Ⅱ ( Angiotensin Ⅱ), the latter is a strong vasoconstrictor, which promotes the increase of blood pressure; on the other hand, ACE can convert bradykinin (bradykinin), which has vasodilation effect, into an inactive fragment, inhibiting the antihypertensive system-activation Peptide release enzyme - kinin system (Kallikrein-Kinin System, KKS), also lead to increased blood pressure. Therefore, inhibiting the activity of ACE can relieve the increase of blood pressure and has the effect of treating hypertension. [0003] Modern research...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K7/08C12P21/06C07K1/18C07K1/20A61K38/10A61P9/12
CPCA61K38/00C07K7/08C12P21/06
Inventor 刘睿盛乃娟吴体智王倩陈晓钰李晓芳吴皓
Owner 南京可馨生物科技有限公司
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